Strain identifier

BacDive ID: 273

Type strain: Yes

Species: Aeromonas salmonicida subsp. smithia

Strain Designation: 138, AS20/1/1

Strain history: CIP <- 1996, NCIB <- CCM <- Austin: strain 138 <- T. Owen: strain AS20/1/1

NCBI tax ID(s): 80745 (subspecies)

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General

@ref: 15607

BacDive-ID: 273

DSM-Number: 21293

keywords: genome sequence, 16S sequence, Bacteria, facultative anaerobe, psychrophilic, Gram-negative, rod-shaped, animal pathogen

description: Aeromonas salmonicida subsp. smithia 138 is a facultative anaerobe, psychrophilic, Gram-negative animal pathogen that was isolated from Ulcer .

NCBI tax id

  • NCBI tax id: 80745
  • Matching level: subspecies

strain history

@refhistory
15607<- CCM <- B. Austin, Department of Brewing and Biological Sciences, University Edinburgh <- T. Owen
122394CIP <- 1996, NCIB <- CCM <- Austin: strain 138 <- T. Owen: strain AS20/1/1

doi: 10.13145/bacdive273.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Aeromonadales
  • family: Aeromonadaceae
  • genus: Aeromonas
  • species: Aeromonas salmonicida subsp. smithia
  • full scientific name: Aeromonas salmonicida subsp. smithia Austin et al. 1989

@ref: 15607

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Aeromonadales

family: Aeromonadaceae

genus: Aeromonas

species: Aeromonas salmonicida subsp. smithia

full scientific name: Aeromonas salmonicida subsp. smithia Austin et al. 1989

strain designation: 138, AS20/1/1

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell shapemotility
66548negative1-2 µmrod-shapedno
122394negativerod-shapedno

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
15607BHI MEDIUM (DSMZ Medium 215)yeshttps://mediadive.dsmz.de/medium/215Name: BHI MEDIUM (DSMZ Medium 215) Composition: Brain heart infusion 37.0 g/l Distilled water
41291MEDIUM 3 - Columbia agaryesColumbia agar (39.000 g);distilled water (1000.000 ml)
66548MacConkey agarno
66548peptone brothyes
66548tryptic soy agar (TSA)yes
122394CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72
122394CIP Medium 3yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=3

culture temp

@refgrowthtypetemperaturerange
15607positivegrowth20psychrophilic
41291positivegrowth22psychrophilic
66548nogrowth30mesophilic
66548positivegrowth4-25
122394positivegrowth5-22psychrophilic
122394nogrowth30mesophilic
122394nogrowth37mesophilic
122394nogrowth41thermophilic

culture pH

@refabilitytypepH
66548nogrowth4
66548nogrowth5

Physiology and metabolism

oxygen tolerance

  • @ref: 122394
  • oxygen tolerance: facultative anaerobe

halophily

@refsaltgrowthtested relationconcentration
66548NaClpositivegrowth0-2 %(w/v)
66548NaClnogrowth3 %(w/v)

observation

@refobservation
66548Cultures on TSA dissociate into rough, smooth, and G-phase colonies, which do not readily produce diffusible brown pigment. In peptone broth cultures are uniformly turbid.
66548With cluster 11, mortalities commenced within 24 h and all the infected stock were dead within 72 h, even after challenge with a minimum of 6.2 x 10-4 cellslfish. External haemorrhages were evident in the vent and on the fins. With dissection, an accumulation of ascitic fluid in the peritoneal cavity, extensive muscle haemorrhaging and liquefaction and gastro-enteritis were apparent. With the addition of tail rot, similar damage was recorded in fish injected with isolates from cluster 14 (includ

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6654816634raffinose-builds acid from
6654817057cellobiose-builds acid from
6654817268myo-inositol-builds acid from
6654817306maltose-builds acid from
6654817716lactose-builds acid from
6654817754glycerol-builds acid from
6654827082trehalose-builds acid from
6654828260galactose-builds acid from
6654829864mannitol-builds acid from
6654830911sorbitol-builds acid from
6654817234glucose+builds acid from
6654817992sucrose+builds acid from
665486731melezitose-carbon source
6654815603L-leucine-carbon source
6654815971L-histidine-carbon source
6654816024D-mannose-carbon source
6654816449dl-alanine-carbon source
6654816467L-arginine-carbon source
6654816857L-threonine-carbon source
6654816988D-ribose-carbon source
6654817115L-serine-carbon source
6654817203L-proline-carbon source
6654817814salicin-carbon source
6654828645beta-D-fructofuranose-carbon source
6654830849L-arabinose-carbon source
6654850144sodium pyruvate-carbon source
6654853258sodium citrate-carbon source
6654862345L-rhamnose-carbon source
6654862983sodium malonate-carbon source
6654863675sodium succinate-carbon source
6654864220sodium glutamate-carbon source
6654865327D-xylose-carbon source
6654891260disodium malate-carbon source
66548115156disodium fumarate-carbon source
665484767elastin-degradation
665484853esculin-degradation
6654815318xanthine-degradation
6654816235guanine-degradation
6654817029chitin-degradation
6654817368hypoxanthine-degradation
6654853423tween 40-degradation
6654853424tween 20-degradation
6654853425tween 60-degradation
6654853426tween 80-degradation
6654861995lecithin-degradation
665485291gelatin+degradation
6654816991dna+degradation
6654828017starch+degradation
6654833697rna+degradation
6654817234glucose-fermentation
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
6837124265gluconate-builds acid from
6837118403L-arabitol-builds acid from
6837118333D-arabitol-builds acid from
6837118287L-fucose-builds acid from
6837128847D-fucose-builds acid from
6837116443D-tagatose-builds acid from
6837162318D-lyxose-builds acid from
6837132528turanose-builds acid from
6837128066gentiobiose-builds acid from
6837117151xylitol-builds acid from
6837116634raffinose-builds acid from
683716731melezitose-builds acid from
6837115443inulin-builds acid from
6837127082trehalose-builds acid from
6837117992sucrose+builds acid from
6837128053melibiose-builds acid from
6837117716lactose-builds acid from
6837117057cellobiose-builds acid from
6837117814salicin-builds acid from
683714853esculin-builds acid from
6837118305arbutin-builds acid from
6837127613amygdalin-builds acid from
6837159640N-acetylglucosamine+builds acid from
68371320061methyl alpha-D-glucopyranoside-builds acid from
6837143943methyl alpha-D-mannoside-builds acid from
6837117924D-sorbitol-builds acid from
6837116899D-mannitol-builds acid from
6837117268myo-inositol-builds acid from
6837116813galactitol-builds acid from
6837162345L-rhamnose-builds acid from
6837117266L-sorbose-builds acid from
6837116024D-mannose-builds acid from
6837115824D-fructose+builds acid from
6837117634D-glucose+builds acid from
6837112936D-galactose-builds acid from
6837174863methyl beta-D-xylopyranoside-builds acid from
6837115963ribitol-builds acid from
6837165328L-xylose-builds acid from
6837165327D-xylose-builds acid from
6837116988D-ribose-builds acid from
6837130849L-arabinose-builds acid from
6837117108D-arabinose-builds acid from
6837117113erythritol-builds acid from
6836925115malate-assimilation
6836917128adipate-assimilation
6836927689decanoate-assimilation
6836924265gluconate-assimilation
6836917306maltose-assimilation
6836959640N-acetylglucosamine-assimilation
6836916899D-mannitol-assimilation
6836916024D-mannose-assimilation
6836930849L-arabinose-assimilation
6836917634D-glucose-assimilation
683695291gelatin+hydrolysis
683694853esculin-hydrolysis
6836916199urea-hydrolysis
6836929016arginine-hydrolysis
6836917634D-glucose+fermentation
6836927897tryptophan-energy source
6836917632nitrate-reduction
6836830849L-arabinose-fermentation
6836827613amygdalin-fermentation
6836828053melibiose-fermentation
6836817992sucrose+fermentation
6836862345L-rhamnose-fermentation
6836830911sorbitol-fermentation
6836817268myo-inositol-fermentation
6836816899D-mannitol-fermentation
6836817634D-glucose+fermentation
683685291gelatin+hydrolysis
6836827897tryptophan-energy source
6836816199urea-hydrolysis
6836816947citrate-assimilation
6836818257ornithine-degradation
6836825094lysine-degradation
6836829016arginine+hydrolysis
12239429864mannitol-fermentation
12239416947citrate-carbon source
1223944853esculin-hydrolysis
12239417234glucose+fermentation
12239417716lactose-fermentation
12239417632nitrate-reduction
12239416301nitrite-reduction
12239415792malonate-assimilation
122394132112sodium thiosulfate-builds gas from
12239417234glucose+degradation

metabolite production

@refChebi-IDmetaboliteproduction
6654816136hydrogen sulfideyes
6654815688acetoinno
6654835581indoleno
6836935581indoleno
6836815688acetoinno
6836835581indoleno
6836816136hydrogen sulfideno
12239435581indoleno

metabolite tests

@refChebi-IDmetabolitemethylred-testvoges-proskauer-testindole test
6654817234glucose-
6654815688acetoin-
6836935581indole-
6836815688acetoin-
6836835581indole-
12239415688acetoin-
12239417234glucose-

enzymes

@refvalueactivityec
66548arginine decarboxylase-4.1.1.19
66548lysine decarboxylase-4.1.1.18
66548ornithine decarboxylase-4.1.1.17
66548phenylalanine deaminase-4.3.1.5
66548beta-galactosidase+3.2.1.23
66548catalase+1.11.1.6
66548cytochrome oxidase+1.9.3.1
66548phosphatase+
66548phosphoamidase+3.9.1.1
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin+3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase+
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase+3.1.3.1
68369cytochrome oxidase-1.9.3.1
68369gelatinase+
68369beta-glucosidase-3.2.1.21
68369urease-3.5.1.5
68369arginine dihydrolase-3.5.3.6
68368cytochrome oxidase+1.9.3.1
68368gelatinase+
68368tryptophan deaminase-4.1.99.1
68368urease-3.5.1.5
68368ornithine decarboxylase-4.1.1.17
68368lysine decarboxylase-4.1.1.18
68368arginine dihydrolase+3.5.3.6
68368beta-galactosidase-3.2.1.23
122394oxidase+
122394beta-galactosidase+3.2.1.23
122394alcohol dehydrogenase-1.1.1.1
122394gelatinase+
122394catalase+1.11.1.6
122394lysine decarboxylase+4.1.1.18
122394ornithine decarboxylase-4.1.1.17
122394phenylalanine ammonia-lyase-4.3.1.24
122394tryptophan deaminase-
122394urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
122394-+++-++-+-++-----+--

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGELGLUMANINOSorRHASACMELAMYARAOX
15607-+--------++----+---+

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPACOX
15607--+---+--------------

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
122394+/----------++---------+-----+/---+----+/-+/-------------

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
122394-+-----------------------------+---------------------------+---------------------------------------

Isolation, sampling and environmental information

isolation

@refsample typehost speciescountryorigin.countrycontinent
15607Ulcer (Rutilus rutilus)Rutilus rutilusUnited KingdomGBREurope
122394Rutilus rutilus, ulcer

isolation source categories

Cat1Cat2
#Infection#Inflammation
#Host#Fishes

taxonmaps

  • @ref: 69479
  • File name: preview.99_485.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_11;96_15;97_15;98_18;99_485&stattab=map
  • Last taxonomy: Aeromonas
  • 16S sequence: AB027544
  • Sequence Identity:
  • Total samples: 313
  • soil counts: 16
  • aquatic counts: 156
  • animal counts: 131
  • plant counts: 10

Safety information

risk assessment

@refpathogenicity animalbiosafety levelbiosafety level comment
15607yes1Risk group (German classification)
1223941Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Aeromonas salmonicida subsp. smithia gene for 16S rRNA, strain: ATCC 49393AB0275441467ena80745
20218Aeromonas salmonicida clone 1 16S ribosomal RNA gene, partial sequence; and 16S-23S ribosomal RNA intergenic spacer, complete sequenceEF373993521ena645
15607Aeromonas salmonicida subsp. smithia 16S rRNA gene, partialAJ0098591505ena80745

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Aeromonas salmonicida CIP 104757GCA_006246305scaffoldncbi645
66792Aeromonas salmonicida strain CIP 104757645.181wgspatric645

GC content

  • @ref: 66548
  • GC-content: 55.4-56.4
  • method: thermal denaturation, midpoint method (Tm)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileyes90.192yes
flagellatedyes92.932no
gram-positiveno98.85no
anaerobicno96.633no
aerobicyes88.011no
halophileno89.358no
spore-formingno95.126no
glucose-utilyes85.789yes
thermophileno99.586yes
glucose-fermentyes87.29no

External links

@ref: 15607

culture collection no.: DSM 21293, ATCC 49393, CCM 4103, CECT 5179, CIP 104757, NCIMB 13210

straininfo link

  • @ref: 69955
  • straininfo: 92494

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
15607Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 21293)https://www.dsmz.de/collection/catalogue/details/culture/DSM-21293
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
41291Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/16701
66548D.A.Austin, D.McIntosh, B.Austin10.1016/S0723-2020(89)80026-8Taxonomy of Fish Associated Aeromonas spp., with the Description of Aeromonas salmonicida subsp. smithia subsp. nov.Syst Appl Microbiol. 11: 277-290 1989
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68368Automatically annotated from API 20E
68369Automatically annotated from API 20NE
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69955Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID92494.1StrainInfo: A central database for resolving microbial strain identifiers
122394Curators of the CIPCollection of Institut Pasteur (CIP 104757)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20104757