Strain identifier

BacDive ID: 2723

Type strain: No

Species: Clostridium tertium

Strain Designation: P.II.Col.54, LRA 231 11 78

Strain history: CIP <- 1993, R. Mercier, bioMérieux, La Balme les Grottes, France: strain LRA 231 11 78 <- Anatylab, Plainview, USA

NCBI tax ID(s): 1559 (species)

« back to strain detail view
For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 8.1 (current version):
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 4.1:
version 4:
version 3:
version 2.1:
version 2:
version 1:
version 8.1 (current version)

General

@ref: 421

BacDive-ID: 2723

DSM-Number: 748

keywords: 16S sequence, Bacteria, anaerobe, mesophilic, Gram-positive, rod-shaped, human pathogen

description: Clostridium tertium P.II.Col.54 is an anaerobe, mesophilic, Gram-positive human pathogen that was isolated from Carcino-sarcomatous tumor of the respiratory tract.

NCBI tax id

  • NCBI tax id: 1559
  • Matching level: species

strain history

@refhistory
421<- K. Kieslich <- ATCC <- NCTC <- M. Robertson <- G.F. Petrie, P.II.Col.54
67770ATCC 19405 <-- NCTC 541 <-- M. Robertson <-- G. F. Petrie (P.II.Col.54).
123481CIP <- 1993, R. Mercier, bioMérieux, La Balme les Grottes, France: strain LRA 231 11 78 <- Anatylab, Plainview, USA

doi: 10.13145/bacdive2723.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Clostridia
  • order: Eubacteriales
  • family: Clostridiaceae
  • genus: Clostridium
  • species: Clostridium tertium
  • full scientific name: Clostridium tertium (Henry 1917) Bergey et al. 1923 (Approved Lists 1980)

  • synonyms

    • @ref: 20215
    • synonym: Bacillus tertius

@ref: 421

domain: Bacteria

phylum: Firmicutes

class: Clostridia

order: Clostridiales

family: Clostridiaceae

genus: Clostridium

species: Clostridium tertium

full scientific name: Clostridium tertium (Henry 1917) Bergey et al. 1923

strain designation: P.II.Col.54, LRA 231 11 78

type strain: no

Morphology

cell morphology

  • @ref: 123481
  • gram stain: positive
  • cell shape: rod-shaped
  • motility: no

colony morphology

  • @ref: 123481

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
421CHOPPED MEAT MEDIUM WITH CARBOHYDRATES (DSMZ Medium 110)yeshttps://mediadive.dsmz.de/medium/110Name: CHOPPED MEAT MEDIUM WITH CARBOHYDRATES (DSMZ Medium 110) Composition: Ground beef 500.0 g/l Casitone 30.0 g/l Agar 15.0 g/l K2HPO4 5.0 g/l Yeast extract 5.0 g/l D-Glucose 4.0 g/l Starch 1.0 g/l Maltose 1.0 g/l Cellobiose 1.0 g/l L-Cysteine HCl 0.5 g/l Ethanol 0.19 g/l Vitamin K3 0.05 g/l Hemin 0.005 g/l Sodium resazurin 0.0005 g/l Vitamin K1 NaOH Distilled water
37030MEDIUM 6 - Columbia agar with 10 % horse bloodyesDistilled water make up to (1000.000 ml);Columbia agar (39.000 g);Horseblood (100.000 ml)
123481CIP Medium 6yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=6
123481CIP Medium 20yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=20

culture temp

@refgrowthtypetemperaturerange
421positivegrowth37mesophilic
37030positivegrowth37mesophilic
44723positivegrowth37mesophilic
67770positivegrowth37mesophilic
123481positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
421anaerobe
44723aerobe
44723anaerobe
123481anaerobe

spore formation

  • @ref: 123481
  • spore formation: no

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
12348117108D-arabinose-degradation
12348115824D-fructose+degradation
12348117634D-glucose+degradation
12348165327D-xylose+degradation
12348117057cellobiose+degradation
12348117716lactose+degradation
12348117306maltose-degradation
12348117814salicin-degradation
12348117992sucrose+degradation
1234814853esculin+hydrolysis
12348117632nitrate-reduction
12348116301nitrite-reduction
12348117632nitrate+respiration

metabolite production

  • @ref: 123481
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase+3.2.1.23
68382alpha-galactosidase+3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase-3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase-3.1.3.1
123481oxidase-
123481beta-galactosidase+3.2.1.23
123481gelatinase-
123481amylase-
123481DNase+
123481caseinase-3.4.21.50
123481catalase-1.11.1.6
123481tween esterase+
123481lecithinase-
123481lipase-
123481protease-
123481urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
123481--++------++++------

Isolation, sampling and environmental information

isolation

  • @ref: 67770
  • sample type: Carcino-sarcomatous tumor of the respiratory tract

taxonmaps

  • @ref: 69479
  • File name: preview.99_3264.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_34;96_1253;97_2035;98_2501;99_3264&stattab=map
  • Last taxonomy: Clostridium tertium
  • 16S sequence: AB689162
  • Sequence Identity:
  • Total samples: 7812
  • soil counts: 574
  • aquatic counts: 386
  • animal counts: 6806
  • plant counts: 46

Safety information

risk assessment

@refpathogenicity humanpathogenicity animalbiosafety levelbiosafety level comment
421yesyes2Risk group (German classification)
1234812Risk group (French classification)

Sequence information

16S sequences

  • @ref: 67770
  • description: Clostridium tertium gene for 16S ribosomal RNA, partial sequence, strain: JCM 3813
  • accession: AB689162
  • length: 1477
  • database: ena
  • NCBI tax ID: 1559

External links

@ref: 421

culture collection no.: DSM 748, ATCC 19405, NCTC 541, CCUG 4219, JCM 3813, BCRC 10650, IAM 14195, NCIMB 541, CIP 103696, JCM 11009

straininfo link

  • @ref: 72251
  • straininfo: 92818

Reference

@idauthorscataloguedoi/urltitle
421Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 748)https://www.dsmz.de/collection/catalogue/details/culture/DSM-748
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
37030Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/15521
44723Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 4219)https://www.ccug.se/strain?id=4219
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
72251Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID92818.1StrainInfo: A central database for resolving microbial strain identifiers
123481Curators of the CIPCollection of Institut Pasteur (CIP 103696)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20103696