Strain identifier
BacDive ID: 2519
Type strain: 
Species: Caloramator proteoclasticus
Strain Designation: U
Strain history: <- S. Tarlera; U <- S. Tarlera {1994}
NCBI tax ID(s): 1121262 (strain), 53342 (species)
version 8.1 (current version)
General
@ref: 3808
BacDive-ID: 2519
DSM-Number: 10124
keywords: genome sequence, 16S sequence, Bacteria, anaerobe, spore-forming, thermophilic, motile
description: Caloramator proteoclasticus U is an anaerobe, spore-forming, thermophilic bacterium that was isolated from from UASB reactor treating cheese whey.
NCBI tax id
| NCBI tax id | Matching level |
|---|---|
| 1121262 | strain |
| 53342 | species |
strain history
- @ref: 3808
- history: <- S. Tarlera; U <- S. Tarlera {1994}
doi: 10.13145/bacdive2519.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacillota
- domain: Bacteria
- phylum: Bacillota
- class: Clostridia
- order: Eubacteriales
- family: Clostridiaceae
- genus: Caloramator
- species: Caloramator proteoclasticus
- full scientific name: Caloramator proteoclasticus Tarlera et al. 1997
@ref: 3808
domain: Bacteria
phylum: Firmicutes
class: Clostridia
order: Clostridiales
family: Clostridiaceae
genus: Caloramator
species: Caloramator proteoclasticus
full scientific name: Caloramator proteoclasticus Tarlera et al. 1997 emend. Ogg and Patel 2011
strain designation: U
type strain: yes
Morphology
cell morphology
| @ref | motility | confidence | gram stain |
|---|---|---|---|
| 69480 | yes | 94.704 | |
| 69480 | 99.731 | positive |
Culture and growth conditions
culture medium
- @ref: 3808
- name: CALORAMATOR PROTEOCLASTICUS MEDIUM (DSMZ Medium 788)
- growth: yes
- link: https://mediadive.dsmz.de/medium/788
- composition: Name: CALORAMATOR MEDIUM (DSMZ Medium 788) Composition: D-Glucose 4.98504 g/l NH4Cl 2.39282 g/l Na2CO3 1.49551 g/l Na2HPO4 x 2 H2O 0.518445 g/l KH2PO4 0.408774 g/l MgCl2 x 6 H2O 0.398804 g/l Na2S x 9 H2O 0.299103 g/l CaCl2 x 2 H2O 0.199402 g/l Yeast extract 0.199402 g/l HCl 0.00249252 g/l FeCl2 x 4 H2O 0.00149551 g/l Sodium resazurin 0.000498504 g/l NaOH 0.000498504 g/l Pyridoxine hydrochloride 0.000299103 g/l Nicotinic acid 0.000199402 g/l Thiamine-HCl x 2 H2O 0.000199402 g/l CoCl2 x 6 H2O 0.000189432 g/l MnCl2 x 4 H2O 9.97009e-05 g/l Calcium pantothenate 9.97009e-05 g/l Vitamin B12 9.97009e-05 g/l p-Aminobenzoic acid 7.97607e-05 g/l ZnCl2 6.97906e-05 g/l Na2MoO4 x 2 H2O 3.58923e-05 g/l NiCl2 x 6 H2O 2.39282e-05 g/l D-(+)-biotin 1.99402e-05 g/l H3BO3 5.98205e-06 g/l Na2WO4 x 2 H2O 3.98804e-06 g/l Na2SeO3 x 5 H2O 2.99103e-06 g/l CuCl2 x 2 H2O 1.99402e-06 g/l Distilled water
culture temp
| @ref | growth | type | temperature | range | confidence |
|---|---|---|---|---|---|
| 3808 | positive | growth | 55 | thermophilic | |
| 69480 | thermophilic | 99.999 |
Physiology and metabolism
oxygen tolerance
| @ref | oxygen tolerance | confidence |
|---|---|---|
| 3808 | anaerobe | |
| 69480 | anaerobe | 99.999 |
spore formation
| @ref | spore formation | confidence |
|---|---|---|
| 69481 | yes | 100 |
| 69480 | yes | 100 |
Isolation, sampling and environmental information
isolation
- @ref: 3808
- sample type: from UASB reactor treating cheese whey
- country: Uruguay
- origin.country: URY
- continent: Middle and South America
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Engineered | #Biodegradation | #Anaerobic digestor |
| #Engineered | #Food production | #Dairy product |
Safety information
risk assessment
- @ref: 3808
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 3808
- description: C. proteolyticus DNA for 16 ribosomal RNA gene
- accession: X90488
- length: 1420
- database: ena
- NCBI tax ID: 53342
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Caloramator proteoclasticus DSM 10124 | GCA_900129265 | scaffold | ncbi | 1121262 |
| 66792 | Caloramator proteoclasticus DSM 10124 | 1121262.4 | wgs | patric | 1121262 |
| 66792 | Caloramator proteoclasticus DSM 10124 | 2582581267 | draft | img | 1121262 |
GC content
- @ref: 3808
- GC-content: 31
Genome-based predictions
predictions
| trait | prediction | confidence | training_data |
|---|---|---|---|
| spore-forming | yes | 100 | no |
| motile | yes | 79.792 | no |
| flagellated | no | 71.279 | no |
| gram-positive | yes | 60.42 | no |
| anaerobic | yes | 99.472 | yes |
| aerobic | no | 97.676 | yes |
| halophile | no | 87.574 | no |
| spore-forming | yes | 85.955 | no |
| thermophile | yes | 99.714 | yes |
| glucose-util | yes | 88.679 | no |
| glucose-ferment | yes | 61.897 | no |
External links
@ref: 3808
culture collection no.: DSM 10124
straininfo link
- @ref: 72054
- straininfo: 48756
literature
| topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
|---|---|---|---|---|---|---|---|---|
| Phylogeny | 9226895 | Caloramator proteoclasticus sp. nov., a new moderately thermophilic anaerobic proteolytic bacterium. | Tarlera S, Muxi L, Soubes M, Stams AJ | Int J Syst Bacteriol | 10.1099/00207713-47-3-651 | 1997 | Bacteria, Anaerobic/*classification/*genetics/ultrastructure, Bacterial Proteins/metabolism, Cell Division, DNA, Bacterial/analysis, Fermentation, Hot Temperature, Microscopy, Electron, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/analysis, Sewage/*microbiology | Stress |
| Phylogeny | 12361295 | Development of fluorescent adjacent hybridization probes and their application in real-time PCR for the simultaneous detection and identification of Fervidobacterium and Caloramator. | Connolly GR, Patel BKC | Int J Syst Evol Microbiol | 10.1099/00207713-52-5-1837 | 2002 | Bacteria, Anaerobic/classification/*genetics/isolation & purification, Base Sequence, Carbocyanines, DNA Primers, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Fluorescein-5-isothiocyanate, *Fluorescent Dyes, Molecular Probe Techniques, Molecular Sequence Data, Nucleic Acid Hybridization, *Oligonucleotide Probes, Polymerase Chain Reaction, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Sequence Homology, Nucleic Acid, Species Specificity | Genetics |
Reference
| @id | authors | catalogue | doi/url | title |
|---|---|---|---|---|
| 3808 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 10124) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-10124 | |
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |
| 69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |
| 69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | 10.21203/rs.3.rs-2527258/v1 | Predictions based on the model GenomeNet Sporulation v. 1 | |
| 72054 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID48756.1 | StrainInfo: A central database for resolving microbial strain identifiers |