Strain identifier

BacDive ID: 24804

Type strain: Yes

Species: Kallotenue papyrolyticum

Strain Designation: JKG1

Strain history: J. Dodsworth and B. P. Hedlund JKG1.

NCBI tax ID(s): 1325125 (species)

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General

@ref: 21295

BacDive-ID: 24804

DSM-Number: 26889

keywords: genome sequence, 16S sequence, Bacteria, aerobe, thermophilic, Gram-negative, motile

description: Kallotenue papyrolyticum JKG1 is an aerobe, thermophilic, Gram-negative bacterium that was isolated from lignocellulosic enrichment incubated in a hot spring.

NCBI tax id

  • NCBI tax id: 1325125
  • Matching level: species

strain history

@refhistory
21295<- B. Hedlund, School Life Sci., Univ. Nevada, Las Vegas, USA; JKG1 <- J. Cole
67770J. Dodsworth and B. P. Hedlund JKG1.

doi: 10.13145/bacdive24804.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/chloroflexota
  • domain: Bacteria
  • phylum: Chloroflexota
  • class: Chloroflexia
  • order: Kallotenuales
  • family: Kallotenuaceae
  • genus: Kallotenue
  • species: Kallotenue papyrolyticum
  • full scientific name: Kallotenue papyrolyticum Cole et al. 2013

@ref: 21295

domain: Bacteria

phylum: Chloroflexi

class: Chloroflexia

order: Kallotenuaceae, not assigned to order

family: Kallotenuaceae

genus: Kallotenue

species: Kallotenue papyrolyticum

full scientific name: Kallotenue papyrolyticum Cole et al. 2013 emend. Nouioui et al. 2018

strain designation: JKG1

type strain: yes

Morphology

cell morphology

  • @ref: 31142
  • gram stain: negative
  • motility: yes

colony morphology

  • @ref: 21295
  • incubation period: 2-3 days

Culture and growth conditions

culture medium

  • @ref: 21295
  • name: KALLOTENUE MEDIUM (DSMZ Medium 1525)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/1525
  • composition: Name: KALLOTENUE MEDIUM (DSMZ Medium 1525) Composition: HEPES buffer 3.53116 g/l D-Glucose 1.97824 g/l Trypticase peptone 0.98912 g/l NaNO3 0.682493 g/l Na2HPO4 0.108803 g/l KNO3 0.098912 g/l CaSO4 x 2 H2O 0.0593472 g/l MgSO4 x 7 H2O 0.0296736 g/l NH4Cl 0.0267062 g/l Nitrilotriacetic acid 0.0148368 g/l NaCl 0.0098912 g/l MnSO4 x H2O 0.0049456 g/l CoSO4 x 7 H2O 0.00178042 g/l ZnSO4 x 7 H2O 0.00178042 g/l CaCl2 x 2 H2O 0.00098912 g/l FeSO4 x 7 H2O 0.00098912 g/l NiCl2 x 6 H2O 0.000296736 g/l AlK(SO4)2 x 12 H2O 0.000197824 g/l H3BO3 9.8912e-05 g/l Na2MoO4 x 2 H2O 9.8912e-05 g/l Pyridoxine hydrochloride 9.8912e-05 g/l CuSO4 x 5 H2O 9.8912e-05 g/l p-Aminobenzoic acid 4.9456e-05 g/l Calcium D-(+)-pantothenate 4.9456e-05 g/l Nicotinic acid 4.9456e-05 g/l Riboflavin 4.9456e-05 g/l Thiamine HCl 4.9456e-05 g/l (DL)-alpha-Lipoic acid 4.9456e-05 g/l Biotin 1.97824e-05 g/l Folic acid 1.97824e-05 g/l Na2WO4 x 2 H2O 3.95648e-06 g/l Na2SeO3 x 5 H2O 2.96736e-06 g/l Vitamin B12 9.8912e-07 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
21295positivegrowth55thermophilic
31142positivegrowth45-65thermophilic
31142positiveoptimum55thermophilic
67770positivegrowth55thermophilic

culture pH

@refabilitytypepHPH range
31142positivegrowth5.6-9.0alkaliphile
31142positiveoptimum7.5

Physiology and metabolism

oxygen tolerance

  • @ref: 31142
  • oxygen tolerance: aerobe

spore formation

  • @ref: 31142
  • spore formation: no

halophily

  • @ref: 31142
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 0-2 %

observation

  • @ref: 67770
  • observation: quinones: MK-9(H6)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3114230089acetate+carbon source
3114222599arabinose+carbon source
3114217057cellobiose+carbon source
3114216947citrate+carbon source
3114217234glucose+carbon source
3114217754glycerol+carbon source
3114224996lactate+carbon source
3114215361pyruvate+carbon source
3114217992sucrose+carbon source
3114218222xylose+carbon source

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentlatitudelongitude
21295lignocellulosic enrichment incubated in a hot springNevada, Great Boiling SpringUSAUSANorth America40.6614-119.366
67770Cellulolytic enrichment incubated in the water column of Great Boiling SpringNVUSAUSANorth America

isolation source categories

Cat1Cat2Cat3
#Engineered#Laboratory#Lab enrichment
#Environmental#Aquatic#Thermal spring
#Condition#Thermophilic (>45°C)

taxonmaps

  • @ref: 69479
  • File name: preview.99_424.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_105;96_287;97_317;98_352;99_424&stattab=map
  • Last taxonomy: Kallotenue papyrolyticum subclade
  • 16S sequence: JX848544
  • Sequence Identity:
  • Total samples: 1834
  • soil counts: 749
  • aquatic counts: 358
  • animal counts: 661
  • plant counts: 66

Safety information

risk assessment

  • @ref: 21295
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 21295
  • description: Kallotenue papyrolyticum strain JKG1 16S ribosomal RNA gene, partial sequence
  • accession: JX848544
  • length: 1355
  • database: ena
  • NCBI tax ID: 1325125

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Kallotenue papyrolyticum JKG12540341237draftimg1325125
67770Kallotenue papyrolyticum JKG1GCA_000526415contigncbi1325125

GC content

@refGC-contentmethod
2129572.4high performance liquid chromatography (HPLC)
3114272.4
6777065.8genome sequence analysis

External links

@ref: 21295

culture collection no.: DSM 26889, JCM 19132

straininfo link

  • @ref: 88626
  • straininfo: 404097

literature

  • topic: Phylogeny
  • Pubmed-ID: 23950149
  • title: Kallotenue papyrolyticum gen. nov., sp. nov., a cellulolytic and filamentous thermophile that represents a novel lineage (Kallotenuales ord. nov., Kallotenuaceae fam. nov.) within the class Chloroflexia.
  • authors: Cole JK, Gieler BA, Heisler DL, Palisoc MM, Williams AJ, Dohnalkova AC, Ming H, Yu TT, Dodsworth JA, Li WJ, Hedlund BP
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.053348-0
  • year: 2013
  • mesh: Cellulose/*metabolism, Chloroflexi/*classification/genetics/isolation & purification, DNA, Bacterial/genetics, Fatty Acids/chemistry, Hot Springs/*microbiology, Molecular Sequence Data, Nevada, Peptidoglycan/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Vitamin K 2/analogs & derivatives/chemistry
  • topic2: Genetics

Reference

@idauthorstitledoi/urlcatalogueID_cross_referencepubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
21295Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-26889Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 26889)
31142Barberan A, Caceres Velazquez H, Jones S, Fierer N.Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information10.1128/mSphere.00237-172747028776041
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/
88626Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID404097.1