General

@ref: 21239

BacDive-ID: 24723

DSM-Number: 21409

keywords: 16S sequence, Bacteria, thermophilic

description: Cecembia calidifontis TU-13 is a thermophilic bacterium that was isolated from water from a hot spring runoff.

NCBI tax id

• NCBI tax id: 1187080
• Matching level: species

strain history

• @ref: 21239
• history: <- M. da Costa, Univ. Coimbra, Portugal; TU-13 <- L. Albuquerque

doi: 10.13145/bacdive24723.20230509.8.1

Name and taxonomic classification

LPSN

• @ref: 20215
• description: domain/bacteria
• keyword: phylum/bacteroidota
• domain: Bacteria
• phylum: Bacteroidota
• class: Cytophagia
• order: Cytophagales
• family: Cyclobacteriaceae
• genus: Cecembia
• species: Cecembia calidifontis
• full scientific name: Cecembia calidifontis Albuquerque et al. 2013

@ref: 21239

domain: Bacteria

phylum: Bacteroidetes

class: Cytophagia

order: Cytophagales

family: Cyclobacteriaceae

genus: Cecembia

species: Cecembia calidifontis

full scientific name: Cecembia calidifontis Albuquerque et al. 2013

strain designation: TU-13

type strain: no

Culture and growth conditions

culture medium

• @ref: 21239
• name: MODIFIED THERMUS 162 MEDIUM (DSMZ Medium 630)
• growth: yes
• link: https://mediadive.dsmz.de/medium/630
• composition: Name: MODIFIED THERMUS 162 MEDIUM (DSMZ Medium 630) Composition: Agar 28.0 g/l Na2HPO4 x 12 H2O 4.3 g/l Yeast extract 2.5 g/l Tryptone 2.5 g/l KH2PO4 0.544 g/l MgCl2 x 6 H2O 0.2 g/l CaSO4 x 2 H2O 0.04 g/l Nitrilotriacetic acid 0.0064 g/l Fe(III) citrate 0.00122472 g/l FeCl2 x 4 H2O 0.0005 g/l MnCl2 x 4 H2O 0.00025 g/l CoCl2 x 4 H2O 0.00015 g/l CuCl2 x 2 H2O 2.5e-05 g/l Na2MoO4 x 2 H2O 2.5e-05 g/l H3BO3 1e-05 g/l NiCl2 x 6 H2O 1e-05 g/l Distilled water

culture temp

• @ref: 21239
• growth: positive
• type: growth
• temperature: 45
• range: thermophilic

Isolation, sampling and environmental information

isolation

• @ref: 21239
• sample type: water from a hot spring runoff
• geographic location: Azores, Island of São Miguel, Furnas
• country: Portugal
• origin.country: PRT
• continent: Europe

isolation source categories

Safety information

risk assessment

• @ref: 21239
• biosafety level: 1
• biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

• @ref: 21239
• description: Cecembia calidifontis partial 16S rRNA gene, strain TU-13
• accession: HE806327
• length: 1499
• database: ena
• NCBI tax ID: 1187080

GC content

• @ref: 21239
• GC-content: 40.8
• method: high performance liquid chromatography (HPLC)

External links

@ref: 21239

culture collection no.: DSM 21409, LMG 24595

straininfo link

• @ref: 88553
• straininfo: 406571

literature

• topic: Phylogeny
• Pubmed-ID: 22843720
• title: Cecembia calidifontis sp. nov., isolated from a hot spring runoff, and emended description of the genus Cecembia.
• authors: Albuquerque L, Tiago I, Nobre MF, Verissimo A, da Costa MS
• journal: Int J Syst Evol Microbiol
• DOI: 10.1099/ijs.0.044537-0
• year: 2012
• mesh: Azores, Bacterial Typing Techniques, Bacteroidetes/*classification/genetics/isolation & purification, Base Composition, DNA, Bacterial/genetics, Fatty Acids/analysis, Hot Springs/*microbiology, Molecular Sequence Data, Phosphatidylethanolamines/analysis, *Phylogeny, RNA, Ribosomal, 16S/genetics, Random Amplified Polymorphic DNA Technique, Sequence Analysis, DNA, Vitamin K 2/analogs & derivatives/analysis, *Water Microbiology
• topic2: Genetics

Reference