General

@ref: 21342

BacDive-ID: 24719

DSM-Number: 27333

keywords: 16S sequence, Bacteria, anaerobe, mesophilic

description: Ercella succinigenes ZWB is an anaerobe, mesophilic bacterium that was isolated from sludge from a biogas desulphurisation bioreactor.

NCBI tax id

• NCBI tax id: 1337094
• Matching level: species

strain history

doi: 10.13145/bacdive24719.20230509.8.1

Name and taxonomic classification

LPSN

• @ref: 20215
• description: domain/bacteria
• keyword: phylum/bacillota
• domain: Bacteria
• phylum: Bacillota
• class: Clostridia
• order: Eubacteriales
• family: Oscillospiraceae
• genus: Ercella
• species: Ercella succinigenes
• full scientific name: Ercella succinigenes van Gelder et al. 2014

@ref: 21342

domain: Bacteria

phylum: Firmicutes

class: Clostridia

order: Clostridiales

family: Oscillospiraceae

genus: Ercella

species: Ercella succinigenes

full scientific name: Ercella succinigenes van Gelder et al. 2014

strain designation: ZWB

type strain: yes

Morphology

colony morphology

• @ref: 21342
• incubation period: 2-3 days

Culture and growth conditions

culture medium

• @ref: 21342
• name: SYNTROPHOBACTER (MPOB) MEDIUM (DSMZ Medium 684)
• growth: yes
• link: https://mediadive.dsmz.de/medium/684
• composition: Name: SYNTROPHOBACTER (MPOB) MEDIUM (DSMZ Medium 684; with strain-specific modifications) Composition: Glycerol 2.29312 g/l Na2CO3 1.49551 g/l Na2HPO4 x 2 H2O 0.528415 g/l Na2S x 9 H2O 0.498504 g/l KH2PO4 0.408774 g/l NH4Cl 0.299103 g/l NaCl 0.299103 g/l Yeast extract 0.199402 g/l CaCl2 x 2 H2O 0.109671 g/l MgCl2 x 6 H2O 0.0997009 g/l HCl 0.00249252 g/l FeCl2 x 4 H2O 0.00149551 g/l Sodium resazurin 0.000498504 g/l NaOH 0.000498504 g/l CoCl2 x 6 H2O 0.000189432 g/l Pyridoxine hydrochloride 9.97009e-05 g/l MnCl2 x 4 H2O 9.97009e-05 g/l ZnCl2 6.97906e-05 g/l p-Aminobenzoic acid 4.98504e-05 g/l Calcium D-(+)-pantothenate 4.98504e-05 g/l Nicotinic acid 4.98504e-05 g/l Riboflavin 4.98504e-05 g/l Thiamine HCl 4.98504e-05 g/l (DL)-alpha-Lipoic acid 4.98504e-05 g/l Na2MoO4 x 2 H2O 3.58923e-05 g/l NiCl2 x 6 H2O 2.39282e-05 g/l Folic acid 1.99402e-05 g/l Biotin 1.99402e-05 g/l H3BO3 5.98205e-06 g/l Na2WO4 x 2 H2O 3.98804e-06 g/l Na2SeO3 x 5 H2O 2.99103e-06 g/l CuCl2 x 2 H2O 1.99402e-06 g/l Vitamin B12 9.97009e-07 g/l Distilled water

culture temp

Physiology and metabolism

oxygen tolerance

• @ref: 21342
• oxygen tolerance: anaerobe

compound production

• @ref: 21342
• compound: succinate

Isolation, sampling and environmental information

isolation

isolation source categories

Safety information

risk assessment

• @ref: 21342
• biosafety level: 1
• biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

• @ref: 21342
• description: Ercella succinigenes partial 16S rRNA gene, type strain ZWBT, clone 4
• accession: HG003571
• length: 1376
• database: ena
• NCBI tax ID: 1337094

GC content

• @ref: 21342
• GC-content: 37.6
• method: high performance liquid chromatography (HPLC)

External links

@ref: 21342

culture collection no.: DSM 27333, JCM 19283

straininfo link

• @ref: 88549
• straininfo: 403121

literature

• topic: Phylogeny
• Pubmed-ID: 24776531
• title: Ercella succinigenes gen. nov., sp. nov., an anaerobic succinate-producing bacterium.
• authors: van Gelder AH, Sousa DZ, Rijpstra WIC, Damste JSS, Stams AJM, Sanchez-Andrea I
• journal: Int J Syst Evol Microbiol
• DOI: 10.1099/ijs.0.058966-0
• year: 2014
• mesh: Bacterial Typing Techniques, Base Composition, *Bioreactors, DNA, Bacterial/genetics, Fatty Acids/chemistry, Gram-Negative Anaerobic Straight, Curved, and Helical Rods/*classification/genetics/isolation & purification, Molecular Sequence Data, Netherlands, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sewage/*microbiology, Succinic Acid/*metabolism
• topic2: Genetics

Reference