Strain identifier
BacDive ID: 24648
Type strain: 
Species: Moorella stamsii
Strain Designation: E3-O
Strain history: <- J. Alves, Univ. Minho, Braga, Portugal; E3-O <- J. Alves {2012}
NCBI tax ID(s): 1266720 (species)
version 8.1 (current version)
General
@ref: 21025
BacDive-ID: 24648
DSM-Number: 26217
keywords: genome sequence, 16S sequence, Bacteria, anaerobe, thermophilic
description: Moorella stamsii E3-O is an anaerobe, thermophilic bacterium that was isolated from anaerobic suspended sludge of a thermophilic municipal solid waste digester.
NCBI tax id
- NCBI tax id: 1266720
- Matching level: species
strain history
- @ref: 21025
- history: <- J. Alves, Univ. Minho, Braga, Portugal; E3-O <- J. Alves {2012}
doi: 10.13145/bacdive24648.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacillota
- domain: Bacteria
- phylum: Bacillota
- class: Clostridia
- order: Thermoanaerobacterales
- family: Thermoanaerobacteraceae
- genus: Moorella
- species: Moorella stamsii
- full scientific name: Moorella stamsii Alves et al. 2013
@ref: 21025
domain: Bacteria
phylum: Firmicutes
class: Clostridia
order: Thermoanaerobacterales
family: Thermoanaerobacteraceae
genus: Moorella
species: Moorella stamsii
full scientific name: Moorella stamsii Alves et al. 2013
strain designation: E3-O
type strain: yes
Culture and growth conditions
culture medium
- @ref: 21025
- name: SYNTHROPHOMONAS MEDIUM (DSMZ Medium 212)
- growth: yes
- link: https://mediadive.dsmz.de/medium/212
- composition: Name: SYNTHROPHOMONAS MEDIUM (DSMZ Medium 212; with strain-specific modifications) Composition: Sucrose 6.97906 g/l Na2SO4 2.79163 g/l Na2CO3 1.49551 g/l Trypticase peptone 0.997008 g/l KH2PO4 0.498505 g/l NaCl 0.398803 g/l NH4Cl 0.398803 g/l MgCl2 x 6 H2O 0.329013 g/l Na2S x 9 H2O 0.299103 g/l L-Cysteine HCl x H2O 0.299103 g/l CaCl2 x 2 H2O 0.0498505 g/l HCl 0.00249252 g/l FeCl2 x 4 H2O 0.00149551 g/l Sodium resazurin 0.000498505 g/l NaOH 0.000498504 g/l Pyridoxine hydrochloride 0.000299103 g/l Nicotinic acid 0.000199402 g/l Thiamine-HCl x 2 H2O 0.000199402 g/l CoCl2 x 6 H2O 0.000189432 g/l Vitamin B12 9.97009e-05 g/l Calcium pantothenate 9.97009e-05 g/l MnCl2 x 4 H2O 9.97009e-05 g/l p-Aminobenzoic acid 7.97607e-05 g/l ZnCl2 6.97906e-05 g/l Na2MoO4 x 2 H2O 3.58923e-05 g/l NiCl2 x 6 H2O 2.39282e-05 g/l D-(+)-biotin 1.99402e-05 g/l H3BO3 5.98205e-06 g/l Na2WO4 x 2 H2O 3.98804e-06 g/l Na2SeO3 x 5 H2O 2.99103e-06 g/l CuCl2 x 2 H2O 1.99402e-06 g/l Distilled water
culture temp
- @ref: 21025
- growth: positive
- type: growth
- temperature: 60
- range: thermophilic
Physiology and metabolism
oxygen tolerance
- @ref: 21025
- oxygen tolerance: anaerobe
Isolation, sampling and environmental information
isolation
- @ref: 21025
- sample type: anaerobic suspended sludge of a thermophilic municipal solid waste digester
- geographic location: Barcelona
- country: Spain
- origin.country: ESP
- continent: Europe
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Engineered | #Biodegradation | #Anaerobic digestor |
| #Engineered | #Waste | #Solid waste |
| #Environmental | #Terrestrial | #Mud (Sludge) |
| #Condition | #Anoxic (anaerobic) |
Safety information
risk assessment
- @ref: 21025
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 21025
- description: Moorella stamsii partial 16S rRNA gene, type strain E3-OT
- accession: HF563589
- length: 1427
- database: ena
- NCBI tax ID: 1266720
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Moorella stamsii DSM 26217 | GCA_002995805 | contig | ncbi | 1266720 |
| 66792 | Moorella stamsii DSM 26217 | 2791355013 | draft | img | 1266720 |
GC content
- @ref: 21025
- GC-content: 53.8
- method: sequence analysis
External links
@ref: 21025
culture collection no.: DSM 26217, CGMCC 1.5181
straininfo link
- @ref: 88488
- straininfo: 402251
literature
- topic: Phylogeny
- Pubmed-ID: 23749275
- title: Moorella stamsii sp. nov., a new anaerobic thermophilic hydrogenogenic carboxydotroph isolated from digester sludge.
- authors: Alves JI, van Gelder AH, Alves MM, Sousa DZ, Plugge CM
- journal: Int J Syst Evol Microbiol
- DOI: 10.1099/ijs.0.050369-0
- year: 2013
- mesh: Bacterial Typing Techniques, Base Composition, Carbohydrates/chemistry, Carbon Monoxide/metabolism, DNA, Bacterial/genetics, Fatty Acids/chemistry, Fermentation, Hot Temperature, Hydrogen-Ion Concentration, Molecular Sequence Data, Moorella/*classification/genetics/isolation & purification, Nucleic Acid Hybridization, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sewage/*microbiology, Spain
- topic2: Stress
Reference
| @id | authors | title | doi/url | catalogue |
|---|---|---|---|---|
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | |
| 21025 | Curators of the DSMZ | https://www.dsmz.de/collection/catalogue/details/culture/DSM-26217 | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 26217) | |
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | |
| 88488 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | StrainInfo: A central database for resolving microbial strain identifiers | 10.60712/SI-ID402251.1 |