Strain identifier
BacDive ID: 24610
Type strain: 
Species: Streptococcus oralis subsp. dentisani
Strain Designation: 7747, Str. 7747
Strain history: <- A. Benitez-Paez, CSISP - Centro Superior de Investigación en Salud Pública, Valencia, Spain; strain 7747 <- R. Cabrera and P. Belda
NCBI tax ID(s): 1458253 (subspecies)
version 8.1 (current version)
General
@ref: 21040
BacDive-ID: 24610
DSM-Number: 27088
keywords: genome sequence, 16S sequence, Bacteria, microaerophile, mesophilic, Gram-positive, coccus-shaped, human pathogen
description: Streptococcus oralis subsp. dentisani 7747 is a microaerophile, mesophilic, Gram-positive human pathogen that was isolated from human caries-free tooth surface.
NCBI tax id
- NCBI tax id: 1458253
- Matching level: subspecies
strain history
- @ref: 21040
- history: <- A. Benitez-Paez, CSISP - Centro Superior de Investigación en Salud Pública, Valencia, Spain; strain 7747 <- R. Cabrera and P. Belda
doi: 10.13145/bacdive24610.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacillota
- domain: Bacteria
- phylum: Bacillota
- class: Bacilli
- order: Lactobacillales
- family: Streptococcaceae
- genus: Streptococcus
- species: Streptococcus oralis subsp. dentisani
- full scientific name: Streptococcus oralis subsp. dentisani (Camelo-Castillo et al. 2014) Jensen et al. 2016
synonyms
- @ref: 20215
- synonym: Streptococcus dentisani
@ref: 21040
domain: Bacteria
phylum: Firmicutes
class: Bacilli
order: Lactobacillales
family: Streptococcaceae
genus: Streptococcus
species: Streptococcus oralis subsp. dentisani
full scientific name: Streptococcus oralis subsp. dentisani (Camelo-Castillo et al. 2014) Jensen et al. 2016
strain designation: 7747, Str. 7747
type strain: yes
Morphology
cell morphology
- @ref: 31158
- gram stain: positive
- cell width: 1.5 µm
- cell shape: coccus-shaped
- motility: no
colony morphology
- @ref: 21040
- type of hemolysis: alpha
- hemolysis ability: 1
- incubation period: 1-2 days
Culture and growth conditions
culture medium
| @ref | name | growth | link | composition |
|---|---|---|---|---|
| 21040 | COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) | yes | https://mediadive.dsmz.de/medium/693 | Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base |
| 21040 | TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) | yes | https://mediadive.dsmz.de/medium/92 | Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water |
culture temp
| @ref | growth | type | temperature | range |
|---|---|---|---|---|
| 21040 | positive | growth | 37 | mesophilic |
| 31158 | positive | growth | 34-40 | |
| 63339 | positive | growth | 37 | mesophilic |
culture pH
- @ref: 31158
- ability: positive
- type: growth
- pH: 6-7.5
Physiology and metabolism
oxygen tolerance
| @ref | oxygen tolerance |
|---|---|
| 21040 | microaerophile |
| 31158 | facultative anaerobe |
| 63339 | microaerophile |
spore formation
- @ref: 31158
- spore formation: no
halophily
- @ref: 31158
- salt: NaCl
- growth: positive
- tested relation: optimum
- concentration: 0.79 %
observation
- @ref: 31158
- observation: aggregates in chains
metabolite utilization
| @ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
|---|---|---|---|---|
| 31158 | 28757 | fructose | + | carbon source |
| 31158 | 28260 | galactose | + | carbon source |
| 31158 | 17716 | lactose | + | carbon source |
| 31158 | 17306 | maltose | + | carbon source |
| 31158 | 37684 | mannose | + | carbon source |
| 31158 | 16634 | raffinose | + | carbon source |
| 31158 | 17992 | sucrose | + | carbon source |
| 68381 | 16988 | D-ribose | - | builds acid from |
| 68381 | 16899 | D-mannitol | - | builds acid from |
| 68381 | 30911 | sorbitol | - | builds acid from |
| 68381 | 17716 | lactose | + | builds acid from |
| 68381 | 27082 | trehalose | - | builds acid from |
| 68381 | 17992 | sucrose | + | builds acid from |
| 68381 | 30849 | L-arabinose | - | builds acid from |
| 68381 | 18333 | D-arabitol | - | builds acid from |
| 68381 | 40585 | alpha-cyclodextrin | - | builds acid from |
| 68381 | 606565 | hippurate | - | hydrolysis |
| 68381 | 28087 | glycogen | - | builds acid from |
| 68381 | 27941 | pullulan | + | builds acid from |
| 68381 | 17306 | maltose | + | builds acid from |
| 68381 | 6731 | melezitose | - | builds acid from |
| 68381 | 320055 | methyl beta-D-glucopyranoside | - | builds acid from |
| 68381 | 16443 | D-tagatose | - | builds acid from |
| 68381 | 16199 | urea | - | hydrolysis |
metabolite production
- @ref: 68381
- Chebi-ID: 15688
- metabolite: acetoin
- production: no
metabolite tests
- @ref: 68381
- Chebi-ID: 15688
- metabolite: acetoin
- voges-proskauer-test: -
enzymes
| @ref | value | activity | ec |
|---|---|---|---|
| 21040 | catalase | - | 1.11.1.6 |
| 21040 | cytochrome-c oxidase | - | 1.9.3.1 |
| 31158 | alkaline phosphatase | + | 3.1.3.1 |
| 31158 | alpha-galactosidase | + | 3.2.1.22 |
| 68381 | urease | - | 3.5.1.5 |
| 68381 | beta-mannosidase | - | 3.2.1.25 |
| 68381 | glycyl tryptophan arylamidase | + | |
| 68381 | pyrrolidonyl arylamidase | - | 3.4.19.3 |
| 68381 | Alanyl-Phenylalanyl-Proline arylamidase | + | |
| 68381 | alkaline phosphatase | + | 3.1.3.1 |
| 68381 | alpha-galactosidase | + | 3.2.1.22 |
| 68381 | beta-glucuronidase | - | 3.2.1.31 |
| 68381 | beta-glucosidase | - | 3.2.1.21 |
API rID32STR
| @ref | ADH Arg | beta GLU | beta GAR | beta GUR | alpha GAL | PAL | RIB | MAN | SOR | LAC | TRE | RAF | SAC | LARA | DARL | CDEX | VP | APPA | beta GAL | PyrA | beta NAG | GTA | HIP | GLYG | PUL | MAL | MEL | MLZ | Mbeta DG | TAG | beta MAN | URE |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 21040 | - | - | + | - | + | + | - | - | - | + | - | - | + | - | - | - | - | + | +/- | - | +/- | + | - | - | + | + | - | - | - | - | - | - |
| 21040 | + | - | + | - | + | + | - | - | - | + | - | + | + | - | - | - | - | + | + | - | + | + | - | - | + | + | +/- | - | - | - | - | - |
Isolation, sampling and environmental information
isolation
| @ref | sample type | geographic location | country | origin.country | continent | sampling date |
|---|---|---|---|---|---|---|
| 21040 | human caries-free tooth surface | Valencia | Spain | ESP | Europe | |
| 25015 | human caries-free tooth surfaces and from the dorsum of the human tongue | |||||
| 63339 | Human tooth,caries free surface | Valencia | Spain | ESP | Europe | 2009 |
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Host | #Human | |
| #Host Body-Site | #Oral cavity and airways | #Tooth |
taxonmaps
- @ref: 69479
- File name: preview.99_1950.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_4;96_4;97_4;98_4;99_1950&stattab=map
- Last taxonomy: Streptococcus
- 16S sequence: HG315101
- Sequence Identity:
- Total samples: 768
- aquatic counts: 11
- animal counts: 749
- plant counts: 8
Safety information
risk assessment
- @ref: 21040
- pathogenicity human: yes, in single cases
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 21040
- description: Streptococcus dentisani partial 16S rRNA gene, strain DSM 27088, isolate 7747
- accession: HG315101
- length: 1535
- database: ena
- NCBI tax ID: 1297857
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Streptococcus oralis subsp. dentisani 7747 CECT 7747 | GCA_000382825 | scaffold | ncbi | 1297857 |
| 66792 | Streptococcus oralis strain FDAARGOS_886 | 1303.919 | complete | patric | 1303 |
GC content
| @ref | GC-content | method |
|---|---|---|
| 21040 | 40.8 | sequence analysis |
| 31158 | 40.8 |
Genome-based predictions
predictions
| trait | prediction | confidence | training_data |
|---|---|---|---|
| motile | no | 94.849 | yes |
| gram-positive | yes | 94.283 | yes |
| anaerobic | no | 94.742 | yes |
| halophile | yes | 76.674 | yes |
| spore-forming | no | 93.986 | yes |
| thermophile | no | 99.527 | no |
| glucose-util | yes | 82.869 | no |
| aerobic | no | 97.035 | yes |
| flagellated | no | 96.186 | yes |
| glucose-ferment | yes | 86.378 | no |
External links
@ref: 21040
culture collection no.: CCUG 66492, DSM 27088, CECT 8312, NBRC 113013
straininfo link
- @ref: 88455
- straininfo: 391794
literature
| topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
|---|---|---|---|---|---|---|---|---|
| Phylogeny | 24006481 | Streptococcus dentisani sp. nov., a novel member of the mitis group. | Camelo-Castillo A, Benitez-Paez A, Belda-Ferre P, Cabrera-Rubio R, Mira A | Int J Syst Evol Microbiol | 10.1099/ijs.0.054098-0 | 2013 | Bacterial Proteins/genetics, Bacterial Typing Techniques, Biofilms, DNA, Bacterial/genetics, Dental Plaque/*microbiology, Genome, Bacterial, Humans, Molecular Sequence Data, *Phylogeny, RNA, Ribosomal, 16S/genetics, RNA, Ribosomal, 23S/genetics, Sequence Analysis, DNA, Streptococcus/*classification/genetics/isolation & purification/metabolism, Superoxide Dismutase/genetics, Tooth/microbiology, alpha-Galactosidase/metabolism | Genetics |
| Phylogeny | 34031726 | Streptococcus shenyangsis sp. nov., a New Species Isolated from the Oropharynx of a Healthy Child from Shenyang China. | Liu D, Xiao C, Li X, Sun Y, Qi H, Zou Y | Curr Microbiol | 10.1007/s00284-021-02500-1 | 2021 | Bacterial Typing Techniques, Child, China, DNA, Bacterial/genetics, Humans, Nucleic Acid Hybridization, *Oropharynx, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Streptococcus/genetics | Pathogenicity |
Reference
| @id | authors | title | doi/url | catalogue | journal | pubmed | ID_cross_reference |
|---|---|---|---|---|---|---|---|
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | ||||
| 21040 | Curators of the DSMZ | https://www.dsmz.de/collection/catalogue/details/culture/DSM-27088 | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 27088) | ||||
| 25015 | A. S. Jensen, C. F.,Kilian, M. | Re-evaluation of the taxonomy of the Mitis group of the genus Streptococcus based on whole genome phylogenetic analyses, and proposed reclassification of Streptococcus dentisani as Streptococcus oralis subsp. dentisani comb. nov., Streptococcus tigurinus as Streptococcus oralis subsp. tigurinus comb. nov., and Streptococcus oligofermentans as a later synonym of Streptococcus cristatus | 10.1099/ijsem.0.001433 | IJSEM 66: 4803-4820 2016 | 27534397 | ||
| 31158 | Barberan A, Caceres Velazquez H, Jones S, Fierer N. | Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information | 10.1128/mSphere.00237-17 | 28776041 | 27486 | ||
| 63339 | Curators of the CCUG | https://www.ccug.se/strain?id=66492 | Culture Collection University of Gothenburg (CCUG) (CCUG 66492) | ||||
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | https://diaspora-project.de/progress.html#genomes | ||||
| 68381 | Automatically annotated from API rID32STR | ||||||
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | MicrobeAtlas 1.0 beta | https://microbeatlas.org/ | ||||
| 88455 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | StrainInfo: A central database for resolving microbial strain identifiers | 10.60712/SI-ID391794.1 |