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Strain identifier

BacDive ID: 24582

Type strain: No

Species: Staphylococcus aureus

Strain Designation: EDCC 5055

Strain history: <- E. Domann, Justus-Liebig-Universität, Institute of Medical Microbiol., Giessen, Germany; EDCC 5055

NCBI tax ID(s): 1280 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 20827

BacDive-ID: 24582

DSM-Number: 28763

keywords: Bacteria, mesophilic

description: Staphylococcus aureus EDCC 5055 is a mesophilic bacterium that was isolated from human, wound, groin.

NCBI tax id

  • NCBI tax id: 1280
  • Matching level: species

strain history: <- E. Domann, Justus-Liebig-Universität, Institute of Medical Microbiol., Giessen, Germany; EDCC 5055

doi: 10.13145/bacdive24582.20221219.7.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Staphylococcaceae
  • genus: Staphylococcus
  • species: Staphylococcus aureus
  • full scientific name: Staphylococcus aureus Rosenbach 1884 (Approved Lists 1980)
  • synonyms

    • @ref: 20215
    • synonym: Staphylococcus aureus subsp. anaerobius

@ref: 20827

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Staphylococcaceae

genus: Staphylococcus

species: Staphylococcus aureus

full scientific name: Staphylococcus aureus Rosenbach 1884

strain designation: EDCC 5055

type strain: no

Morphology

colony morphology

  • @ref: 20827
  • type of hemolysis: beta
  • incubation period: 2-3 days

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
20827COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
20827TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)yeshttps://mediadive.dsmz.de/medium/92Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water

culture temp

  • @ref: 20827
  • growth: positive
  • type: growth
  • temperature: 37
  • range: mesophilic

Physiology and metabolism

antibiogram

@refmediumincubation temperatureincubation timeoxygen conditionPenicillin Goxacillinampicillinticarcillinmezlocillincefalotincefazolincefotaximeaztreonamimipenemtetracyclinechloramphenicolgentamycinamikacinvancomycinerythromycinlincomycinofloxacinnorfloxacincolistinpipemidic acidnitrofurantoinbacitracinpolymyxin bkanamycinneomycindoxycyclineceftriaxoneclindamycinfosfomycinmoxifloxacinlinezolidnystatinquinupristin/dalfopristinteicoplaninpiperacillin/tazobactam
20827Mueller-Hinton Agar371aerob1622182216302620038323026262083230280102222102622-2432163832-3434360281824
20827Mueller-Hinton Agar37aerob2022-242022-241830-32262004034-3630-322622-2420-221030303001024-26181026223616-1838-403432-3434-36028-3018-2024

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6837522599arabinose-fermentation
6837559640N-acetylglucosamine+fermentation
6837517992sucrose+fermentation
6837517632nitrate+reduction
6837517057cellobiose-fermentation
6837516988D-ribose-fermentation
6837516634raffinose-fermentation
6837516899D-mannitol+fermentation
6837527082trehalose+fermentation
6837517716lactose+fermentation
6837517306maltose+fermentation
6837516024D-mannose+fermentation
6837515824D-fructose+fermentation
6837517634D-glucose+fermentation
683754853esculin-hydrolysis
6837518257ornithine-degradation
6837529016arginine+hydrolysis
6837516199urea+hydrolysis

antibiotic resistance

  • @ref: 68375
  • ChEBI: 28368
  • metabolite: novobiocin
  • is antibiotic: yes
  • is resistant: yes
  • resistance conc.: 1.8 µg

metabolite production

  • @ref: 68375
  • Chebi-ID: 15688
  • metabolite: acetoin
  • production: yes

metabolite tests

  • @ref: 68375
  • Chebi-ID: 15688
  • metabolite: acetoin
  • voges-proskauer-test: +

enzymes

@refvalueactivityec
20827catalase+1.11.1.6
20827cytochrome-c oxidase-1.9.3.1
68375beta-glucuronidase-3.2.1.31
68375pyrrolidonyl arylamidase+3.4.19.3
68375alkaline phosphatase+3.1.3.1
68375L-arginine arylamidase-
68375beta-galactosidase-3.2.1.23
68375beta-glucosidase-3.2.1.21
68375ornithine decarboxylase-4.1.1.17
68375arginine dihydrolase+3.5.3.6
68375urease+3.5.1.5

API ID32STA

@refUREADH ArgODCESCGLUFRUMNEMALLACTREMANRAFRIBCELNITVPbeta GALArgAPALPyrANOVOSACNAGTURARAbeta GUR
20827++--+++++++---++--++++++/---
20827++--+++++++---++--++++++/---

Isolation, sampling and environmental information

isolation

  • @ref: 20827
  • sample type: human, wound, groin
  • geographic location: Giessen
  • country: Germany
  • origin.country: DEU
  • continent: Europe

isolation source categories

Cat1Cat2Cat3
#Host#Human
#Host Body-Site#Other#Wound

Safety information

risk assessment

  • @ref: 20827
  • biosafety level: 2
  • biosafety level comment: Risk group (German classification)

External links

@ref: 20827

culture collection no.: DSM 28763

literature

Pubmed-IDtitleauthorsjournalDOIyearmeshtopictopic2
24948548Rifampicin-fosfomycin coating for cementless endoprostheses: antimicrobial effects against methicillin-sensitive Staphylococcus aureus (MSSA) and methicillin-resistant Staphylococcus aureus (MRSA).Alt V, Kirchhof K, Seim F, Hrubesch I, Lips KS, Mannel H, Domann E, Schnettler RActa Biomater10.1016/j.actbio.2014.06.0132014Animals, *Antibiotics, Antitubercular/chemistry/pharmacology, *Bone Wires, *Coated Materials, Biocompatible/chemistry/pharmacology, *Fosfomycin/chemistry/pharmacology, Materials Testing, Methicillin-Resistant Staphylococcus aureus/*growth & development, Prostheses and Implants, Rabbits, *Rifampin/chemistry/pharmacology, Staphylococcal Infections/*prevention & control
27716055TLR9 mediates S. aureus killing inside osteoblasts via induction of oxidative stress.Mohamed W, Domann E, Chakraborty T, Mannala G, Lips KS, Heiss C, Schnettler R, Alt VBMC Microbiol10.1186/s12866-016-0855-82016Biofilms/drug effects, Cell Line, Tumor, DNA, Bacterial/immunology, Flow Cytometry, Humans, Immunity, Innate, Oligodeoxyribonucleotides/immunology/*pharmacology, Onium Compounds/pharmacology, Osteoblasts/*immunology/*microbiology, Oxidative Stress/drug effects/*immunology, Staphylococcal Infections/drug therapy/immunology/microbiology, Staphylococcus aureus/drug effects/*immunology/physiology, Toll-Like Receptor 9/agonists/biosynthesis/*immunology/metabolismPathogenicityStress
28232428Complete Genome and Plasmid Sequences of Staphylococcus aureus EDCC 5055 (DSM 28763), Used To Study Implant-Associated Infections.Mannala GK, Hain T, Sproer C, Bunk B, Overmann J, Alt V, Domann EGenome Announc10.1128/genomeA.01698-162017
35604422Rifampicin restores extracellular organic matrix formation and mineralization of osteoblasts after intracellular Staphylococcus aureus infection.Alagboso FI, Mannala GK, Walter N, Docheva D, Brochhausen C, Alt V, Rupp MBone Joint Res10.1302/2046-3758.115.BJR-2021-0395.R12022

Reference

@idauthorstitledoi/urlcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
20827Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-28763Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 28763)
68375Automatically annotated from API ID32STA