Strain identifier

BacDive ID: 24442

Type strain: Yes

Species: Pseudomonas stutzeri

Strain Designation: HL22-2

Strain history: CIP <- 2014, DSMZ <- F. Xie, Henan Academy Sciences, Henan, China: strain HL22-2 <- Y. Chao

NCBI tax ID(s): 1211807 (species)

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General

@ref: 21145

BacDive-ID: 24442

DSM-Number: 25974

keywords: genome sequence, 16S sequence, Bacteria, mesophilic, Gram-negative, motile, rod-shaped, polysaccharide production

description: Pseudomonas stutzeri HL22-2 is a mesophilic, Gram-negative, motile bacterium that produces polysaccharides and was isolated from phosphate rock powder from a phosphate mining field.

NCBI tax id

  • NCBI tax id: 1211807
  • Matching level: species

strain history

@refhistory
21145<- F. Xie, Henan Key Laboratory of Microbial Engineering, Yapeng Chao; HL22-2 <-
120512CIP <- 2014, DSMZ <- F. Xie, Henan Academy Sciences, Henan, China: strain HL22-2 <- Y. Chao

doi: 10.13145/bacdive24442.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Pseudomonadales
  • family: Pseudomonadaceae
  • genus: Pseudomonas
  • species: Pseudomonas stutzeri
  • full scientific name: Pseudomonas stutzeri (Lehmann and Neumann 1896) Sijderius 1946 (Approved Lists 1980)

  • synonyms

    @refsynonym
    20215Stutzerimonas chloritidismutans
    20215Pseudomonas perfectomarinus
    20215Bacterium stutzeri
    20215Pseudomonas kunmingensis
    20215Pseudomonas chloritidismutans
    20215Stutzerimonas kunmingensis
    20215Stutzerimonas stutzeri
    20215Stutzerimonas perfectomarina
    20215Pseudomonas perfectomarina

@ref: 21145

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Pseudomonadales

family: Pseudomonadaceae

genus: Pseudomonas

species: Pseudomonas kunmingensis

full scientific name: Pseudomonas kunmingensis Xie et al. 2014

strain designation: HL22-2

type strain: yes

Morphology

cell morphology

  • @ref: 120512
  • gram stain: negative
  • cell shape: rod-shaped
  • motility: yes

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
41598MEDIUM 328- for nutrient agaryesDistilled water make up to (1000.000 ml);Agar (15.000 g);Peptone (5.000g);Beef extract (3.000 g)
21145REACTIVATION WITH LIQUID MEDIUM 1 (DSMZ Medium 1a)yesName: REACTIVATION WITH LIQUID MEDIUM 1 (DSMZ Medium 1a) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled waterhttps://mediadive.dsmz.de/medium/1a
120512CIP Medium 328yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=328

culture temp

@refgrowthtypetemperaturerange
21145positivegrowth28mesophilic
41598positivegrowth30mesophilic

Physiology and metabolism

compound production

  • @ref: 21145
  • compound: exopolysaccharide

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6836925115malate+assimilation
6836917128adipate-assimilation
6836927689decanoate+assimilation
6836924265gluconate+assimilation
6836917306maltose+assimilation
6836959640N-acetylglucosamine-assimilation
6836916899D-mannitol+assimilation
6836916024D-mannose-assimilation
6836930849L-arabinose-assimilation
6836917634D-glucose+assimilation
683695291gelatin-hydrolysis
683694853esculin-hydrolysis
6836916199urea-hydrolysis
6836929016arginine-hydrolysis
6836917634D-glucose-fermentation
6836927897tryptophan-energy source
6836917632nitrate+reduction

metabolite production

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
68369cytochrome oxidase+1.9.3.1
68369gelatinase-
68369beta-glucosidase-3.2.1.21
68369urease-3.5.1.5
68369arginine dihydrolase-3.5.3.6

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPACOX
21145+-------+--+-+++-++-+
21145+-------+--+-+++-++-+

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
21145phosphate rock powder from a phosphate mining fieldYunnan Province, suburb of KunmingChinaCHNAsia
120512Phosphate rock powder from a phosphate mining fieldKunming, YunnanChinaCHNAsia

isolation source categories

Cat1Cat2Cat3
#Engineered#Other#Mine
#Environmental#Terrestrial#Geologic

taxonmaps

  • @ref: 69479
  • File name: preview.99_976.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_23;96_61;97_64;98_148;99_976&stattab=map
  • Last taxonomy: Pseudomonas
  • 16S sequence: JQ246444
  • Sequence Identity:
  • Total samples: 243
  • soil counts: 62
  • aquatic counts: 64
  • animal counts: 92
  • plant counts: 25

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
211451Risk group (German classification)
1205121Risk group (French classification)

Sequence information

16S sequences

  • @ref: 21145
  • description: Pseudomonas kunmingensis strain HL22-2 16S ribosomal RNA gene, partial sequence
  • accession: JQ246444
  • length: 1498
  • database: ena
  • NCBI tax ID: 1211807

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Stutzerimonas kunmingensis DSM 25974GCA_900114065scaffoldncbi1211807
66792Pseudomonas kunmingensis strain DSM 259741211807.3wgspatric1211807
66792Pseudomonas kunmingensis DSM 259742663763606draftimg1211807
66792Stutzerimonas kunmingensis DSM 25974GCA_024397575contigncbi1211807

GC content

  • @ref: 21145
  • GC-content: 60.3
  • method: thermal denaturation, midpoint method (Tm)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileyes87.3no
flagellatedyes91.834no
gram-positiveno98.825no
anaerobicno97.566no
aerobicyes91.808no
halophileno78.678no
spore-formingno94.805no
glucose-fermentno92.176no
thermophileno99.742yes
glucose-utilyes91.045yes

External links

@ref: 21145

culture collection no.: DSM 25974, CGMCC 1.12273, CIP 110808

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny24225026Pseudomonas kunmingensis sp. nov., an exopolysaccharide-producing bacterium isolated from a phosphate mine.Xie F, Ma H, Quan S, Liu D, Chen G, Chao Y, Qian SInt J Syst Evol Microbiol10.1099/ijs.0.055632-02013Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Fatty Acids/chemistry, *Mining, Molecular Sequence Data, Nucleic Acid Hybridization, Phosphates, *Phylogeny, Pseudomonas/*classification/genetics/isolation & purification, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Ubiquinone/chemistryGenetics
Phylogeny25550067Pseudomonas songnenensis sp. nov., isolated from saline and alkaline soils in Songnen Plain, China.Zhang L, Pan Y, Wang K, Zhang X, Zhang S, Fu X, Zhang C, Jiang JAntonie Van Leeuwenhoek10.1007/s10482-014-0365-32014Aerobiosis, Alkalies, Bacterial Typing Techniques, China, Cluster Analysis, Cytosol/chemistry, DNA Gyrase/genetics, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, DNA-Directed RNA Polymerases/genetics, Fatty Acids/analysis, Flagella/physiology, Locomotion, Molecular Sequence Data, Nucleic Acid Hybridization, Phospholipids/analysis, Phylogeny, Pigments, Biological/metabolism, Pseudomonas/*classification/genetics/*isolation & purification/physiology, Quinones/analysis, RNA, Ribosomal, 16S/genetics, Salinity, Sequence Analysis, DNA, Sigma Factor/genetics, Soil/chemistry, *Soil MicrobiologyGenetics
Phylogeny25574037Pseudomonas zhaodongensis sp. nov., isolated from saline and alkaline soils.Zhang L, Pan Y, Wang K, Zhang X, Zhang C, Zhang S, Fu X, Jiang JInt J Syst Evol Microbiol10.1099/ijs.0.0000572015Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Fatty Acids/chemistry, Genes, Bacterial, Hydrogen-Ion Concentration, Molecular Sequence Data, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, Pigmentation, Pseudomonas/*classification/genetics/isolation & purification, RNA, Ribosomal, 16S/genetics, Salinity, Sequence Analysis, DNA, Soil/chemistry, *Soil Microbiology, Ubiquinone/chemistryGenetics

Reference

@idauthorstitledoi/urlcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
21145Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-25974Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 25974)
41598Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/30621
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
68369Automatically annotated from API 20NE
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/
120512Curators of the CIPhttps://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20110808Collection of Institut Pasteur (CIP 110808)