Strain identifier

BacDive ID: 24346

Type strain: Yes

Species: Microlunatus flavus

Strain Designation: W6

Strain history: J. Zhang W6.

NCBI tax ID(s): 1036181 (species)

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General

@ref: 20574

BacDive-ID: 24346

DSM-Number: 27985

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, Gram-positive, ovoid-shaped

description: Microlunatus flavus W6 is an aerobe, mesophilic, Gram-positive bacterium that was isolated from soil.

NCBI tax id

  • NCBI tax id: 1036181
  • Matching level: species

strain history

@refhistory
20574<- JCM <- J. Zhang, Beijing Inst. of Technol., School of Life Science; W6
67770J. Zhang W6.

doi: 10.13145/bacdive24346.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Propionibacteriales
  • family: Propionibacteriaceae
  • genus: Microlunatus
  • species: Microlunatus flavus
  • full scientific name: Microlunatus flavus (Zhang et al. 2013) Nouioui et al. 2018

  • synonyms

    • @ref: 20215
    • synonym: Friedmanniella flava

@ref: 20574

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Propionibacteriaceae

genus: Microlunatus

species: Microlunatus flavus

full scientific name: Microlunatus flavus (Zhang et al. 2013) Nouioui et al. 2018

strain designation: W6

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotilityconfidence
30771positive0.65 µm0.65 µmovoid-shapedno
69480no93.811
69480positive100

pigmentation

  • @ref: 30771
  • production: yes

Culture and growth conditions

culture medium

  • @ref: 20574
  • name: R2A MEDIUM (DSMZ Medium 830)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/830
  • composition: Name: R2A MEDIUM (DSMZ Medium 830) Composition: Agar 15.0 g/l Casamino acids 0.5 g/l Starch 0.5 g/l Glucose 0.5 g/l Proteose peptone 0.5 g/l Yeast extract 0.5 g/l K2HPO4 0.3 g/l Na-pyruvate 0.3 g/l MgSO4 x 7 H2O 0.05 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
20574positivegrowth28mesophilic
30771positivegrowth09-32
30771positiveoptimum26.5mesophilic
67770positivegrowth28mesophilic

culture pH

@refabilitytypepHPH range
30771positivegrowth05-09alkaliphile
30771positiveoptimum6.0-7.5

Physiology and metabolism

oxygen tolerance

  • @ref: 30771
  • oxygen tolerance: aerobe

spore formation

@refspore formationconfidence
30771no
69481no99
69480no98.806

halophily

  • @ref: 30771
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 0-1 %

observation

@refobservation
30771aggregates in clumps
67770quinones: MK-9(H4)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3077122599arabinose+carbon source
3077128757fructose+carbon source
3077128260galactose+carbon source
3077117234glucose+carbon source
3077129864mannitol+carbon source
3077116634raffinose+carbon source
3077126546rhamnose+carbon source
3077117992sucrose+carbon source
3077118222xylose+carbon source

enzymes

@refvalueactivityec
30771acid phosphatase+3.1.3.2
30771alkaline phosphatase+3.1.3.1
30771alpha-galactosidase+3.2.1.22
30771catalase+1.11.1.6
30771urease+3.5.1.5

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
20574soilYunnan ProvinceChinaCHNAsia
67770Soil of Yunnan ProvinceChinaCHNAsia

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Terrestrial
  • Cat3: #Soil

Safety information

risk assessment

  • @ref: 20574
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 20574
  • description: Friedmanniella flava strain W6 16S ribosomal RNA gene, partial sequence
  • accession: HQ839787
  • length: 1402
  • database: ena
  • NCBI tax ID: 1036181

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Friedmanniella flava strain CGMCC 4.68561036181.4wgspatric1036181
66792Friedmanniella flavus flava CGMCC 4.68562675903052draftimg1036181
67770Microlunatus flavus CGMCC 4.6856GCA_900110855scaffoldncbi1036181

GC content

@refGC-contentmethod
2057472
6777072thermal denaturation, midpoint method (Tm)
6777073.5genome sequence analysis

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno99no
motileno93.071no
flagellatedno98.483yes
gram-positiveyes88.778no
anaerobicno99.112yes
aerobicyes92.364yes
halophileno95.471yes
spore-formingno80.646yes
thermophileno99.116yes
glucose-utilyes87.637yes
glucose-fermentno86.403no

External links

@ref: 20574

culture collection no.: DSM 27985, CGMCC 4.6856, JCM 17701, KACC 17380

straininfo link

  • @ref: 88272
  • straininfo: 404172

literature

  • topic: Phylogeny
  • Pubmed-ID: 22941301
  • title: Friedmanniella flava sp. nov., a soil actinomycete.
  • authors: Zhang X, Zhang J, Zhang Y, Xin Y, He H
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.043984-0
  • year: 2012
  • mesh: Actinomycetales/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Fatty Acids/analysis, Molecular Sequence Data, Nucleic Acid Hybridization, Peptidoglycan/analysis, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Vitamin K 2/analogs & derivatives/analysis
  • topic2: Genetics

Reference

@idauthorstitledoi/urlcatalogueID_cross_referencepubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
20574Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-27985Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 27985)
30771Barberan A, Caceres Velazquez H, Jones S, Fierer N.Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information10.1128/mSphere.00237-172710228776041
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. MünchPredictions based on the model GenomeNet Sporulation v. 110.21203/rs.3.rs-2527258/v1
88272Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID404172.1