Strain identifier

BacDive ID: 24186

Type strain: Yes

Species: Cryobacterium flavum

Strain Designation: Hh8

Strain history: CGMCC 1.11215 <-- Y. Xin Hh8.

NCBI tax ID(s): 1424659 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 20518

BacDive-ID: 24186

DSM-Number: 26475

keywords: psychrophilic, aerobe, Bacteria, 16S sequence, genome sequence, Gram-positive, rod-shaped

description: Cryobacterium flavum Hh8 is an aerobe, psychrophilic, Gram-positive bacterium that was isolated from glacier ice.

NCBI tax id

  • NCBI tax id: 1424659
  • Matching level: species

strain history

doi: 10.13145/bacdive24186.20220920.7

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Micrococcales
  • family: Microbacteriaceae
  • genus: Cryobacterium
  • species: Cryobacterium flavum
  • full scientific name: Cryobacterium flavum Liu et al. 2012

@ref: 20518

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Microbacteriaceae

genus: Cryobacterium

species: Cryobacterium flavum

full scientific name: Cryobacterium flavum Liu et al. 2012

strain designation: Hh8

type strain: yes

Morphology

cell morphology

  • @ref: 30337
  • gram stain: positive
  • cell length: 0.76 µm
  • cell width: 0.41 µm
  • cell shape: rod-shaped

pigmentation

  • @ref: 30337
  • production: yes

Culture and growth conditions

culture medium

  • @ref: 20518
  • name: PP-MEDIUM (DSMZ Medium 513)
  • growth: yes
  • link: https://bacmedia.dsmz.de/medium/513
  • composition: Name: PP-MEDIUM (DSMZ Medium 513) Composition: Agar 15.0 g/l Polypeptone 10.0 g/l Yeast extract 2.0 g/l MgSO4 x 7 H2O 1.0 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
20518positivegrowth10psychrophilic
30337positivegrowth0-19psychrophilic
67770positivegrowth10psychrophilic

culture pH

  • @ref: 30337
  • ability: positive
  • type: growth
  • pH: 5.5-10.5

Physiology and metabolism

oxygen tolerance

  • @ref: 30337
  • oxygen tolerance: aerobe

halophily

  • @ref: 30337
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 0-3 %

observation

  • @ref: 67770
  • observation: quinones: MK-10, MK-11, MK-12

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3033722599arabinose+carbon source
3033717057cellobiose+carbon source
3033728757fructose+carbon source
3033728260galactose+carbon source
3033717234glucose+carbon source
3033717754glycerol+carbon source
3033737684mannose+carbon source
3033733942ribose+carbon source
303374853esculin+hydrolysis
3033717632nitrate+reduction

enzymes

@refvalueactivityec
30337acid phosphatase+3.1.3.2
30337catalase+1.11.1.6

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
20518glacier iceXingjiang Uygur Autonomous RegionChinaCHNAsia
67770Ice of the No. 1 glacierXingjiang Uygur Autonomous Region, north-west ChinaChinaCHNAsia

isolation source categories

Cat1Cat2Cat3
#Environmental#Terrestrial#Glacier
#Environmental#Aquatic#Ice

Safety information

risk assessment

  • @ref: 20518
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 20518
  • description: Cryobacterium flavum strain Hh8 16S ribosomal RNA gene, partial sequence
  • accession: HQ845195
  • length: 1475
  • database: ena
  • NCBI tax ID: 1424659

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Cryobacterium flavum Hh8GCA_004402625contigncbi1424659
66792Cryobacterium flavum CGMCC 1.11215GCA_900103805scaffoldncbi1424659
66792Cryobacterium flavum strain CGMCC 1.112151424659.3wgspatric1424659
66792Cryobacterium flavum strain Hh81424659.4wgspatric1424659
66792Cryobacterium flavum CGMCC 1.112152663762753draftimg1424659
67770Cryobacterium flavum strain CGMCC 1.11215, whole genome shotgun sequencing projectFNIB00000000ncbi1424659
67770Cryobacterium flavum strain Hh8, whole genome shotgun sequencing projectSOFD00000000ncbi1424659

GC content

@refGC-contentmethod
2051863.7
6777063.7thermal denaturation, midpoint method (Tm)

External links

@ref: 20518

culture collection no.: DSM 26475, CGMCC 1.11215, NBRC 107879, JCM 18568

straininfo link

@refpassport
20218http://www.straininfo.net/strains/871599
20218http://www.straininfo.net/strains/871600

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny21784961Cryobacterium flavum sp. nov. and Cryobacterium luteum sp. nov., isolated from glacier ice.Liu Q, Liu H, Wen Y, Zhou Y, Xin YInt J Syst Evol Microbiol10.1099/ijs.0.033738-02011Actinomycetales/*classification/genetics/*isolation & purification/metabolism, China, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Fatty Acids/metabolism, Ice Cover/*microbiology, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/geneticsMetabolism
Phylogeny23315411Cryobacterium levicorallinum sp. nov., a psychrophilic bacterium isolated from glacier ice.Liu Q, Liu H, Zhang J, Zhou Y, Xin YInt J Syst Evol Microbiol10.1099/ijs.0.046896-02013Actinomycetales/*classification/genetics/metabolism, Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Fatty Acids/analysis, Ice, Ice Cover/*microbiology, Molecular Sequence Data, Nucleic Acid Hybridization, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNAGenetics

Reference

@idauthorstitledoi/urljournalpubmedcatalogueID_cross_reference
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.StrainInfo introduces electronic passports for microorganisms.10.1016/j.syapm.2013.11.002Syst Appl Microbiol. 37: 42-50 201424321274
20518Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-26475Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 26475)
2667810.1099/ijs.0.033738-021784961
30337Barberan A, Caceres Velazquez H, Jones S, Fierer N.Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information10.1128/mSphere.00237-172877604126678
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/