Strain identifier

BacDive ID: 24149

Type strain: No

Species: Leuconostoc gelidum subsp. aenigmaticum

Strain Designation: POKY4-4

Strain history: <- R. Rahkila, University of Helsinki <- E. Merivirta; POKY4-4

NCBI tax ID(s): 1607837 (subspecies)

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General

@ref: 21008

BacDive-ID: 24149

DSM-Number: 27694

keywords: genome sequence, 16S sequence, Bacteria, microaerophile, mesophilic

description: Leuconostoc gelidum subsp. aenigmaticum POKY4-4 is a microaerophile, mesophilic bacterium that was isolated from modified atmosphere-packaged pork chop.

NCBI tax id

  • NCBI tax id: 1607837
  • Matching level: subspecies

strain history

  • @ref: 21008
  • history: <- R. Rahkila, University of Helsinki <- E. Merivirta; POKY4-4

doi: 10.13145/bacdive24149.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Lactobacillaceae
  • genus: Leuconostoc
  • species: Leuconostoc gelidum subsp. aenigmaticum
  • full scientific name: Leuconostoc gelidum subsp. aenigmaticum Rahkila et al. 2014

@ref: 21008

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Leuconostocaceae

genus: Leuconostoc

species: Leuconostoc gelidum subsp. aenigmaticum

full scientific name: Leuconostoc gelidum subsp. aenigmaticum Rahkila et al. 2014

strain designation: POKY4-4

type strain: no

Culture and growth conditions

culture medium

  • @ref: 21008
  • name: MRS MEDIUM (DSMZ Medium 11)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/11
  • composition: Name: MRS MEDIUM (DSMZ Medium 11) Composition: Glucose 20.0 g/l Casein peptone 10.0 g/l Meat extract 10.0 g/l Na-acetate 5.0 g/l Yeast extract 5.0 g/l (NH4)3 citrate 2.0 g/l K2HPO4 2.0 g/l Tween 80 1.0 g/l MgSO4 x 7 H2O 0.2 g/l MnSO4 x H2O 0.05 g/l Distilled water

culture temp

  • @ref: 21008
  • growth: positive
  • type: growth
  • temperature: 25
  • range: mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 21008
  • oxygen tolerance: microaerophile

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate-builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose-builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose+builds acid from32528
68371gentiobiose-builds acid from28066
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371starch-builds acid from28017
68371raffinose+builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose+builds acid from27082
68371sucrose+builds acid from17992
68371melibiose+builds acid from28053
68371lactose-builds acid from17716
68371maltose+builds acid from17306
68371cellobiose+builds acid from17057
68371salicin-builds acid from17814
68371esculin+builds acid from4853
68371arbutin-builds acid from18305
68371amygdalin-builds acid from27613
68371N-acetylglucosamine-builds acid from59640
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol-builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose+builds acid from16024
68371D-fructose+builds acid from15824
68371D-glucose+builds acid from17634
68371D-galactose-builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose+builds acid from65327
68371D-ribose+builds acid from16988
68371L-arabinose+builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754

API 50CHac

@refQGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
21008----+++----+++-----------+-++-+++--+----+---------

Isolation, sampling and environmental information

isolation

  • @ref: 21008
  • sample type: modified atmosphere-packaged pork chop
  • country: Finland
  • origin.country: FIN
  • continent: Europe

isolation source categories

Cat1Cat2Cat3
#Engineered#Food production#Bottled, canned, packed food
#Engineered#Food production#Meat
#Host#Mammals#Suidae (Pig,Swine)

taxonmaps

  • @ref: 69479
  • File name: preview.99_3716.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_22;96_35;97_36;98_457;99_3716&stattab=map
  • Last taxonomy: Leuconostoc gelidum
  • 16S sequence: KF577568
  • Sequence Identity:
  • Total samples: 22220
  • soil counts: 1578
  • aquatic counts: 2226
  • animal counts: 17034
  • plant counts: 1382

Safety information

risk assessment

  • @ref: 21008
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 21008
  • description: Leuconostoc gelidum subsp. aenigmaticum strain POKY4-4 16S ribosomal RNA gene, partial sequence
  • accession: KF577568
  • length: 1542
  • database: ena
  • NCBI tax ID: 1607837

Genome sequences

  • @ref: 66792
  • description: Leuconostoc gelidum subsp. aenigmaticum POKY4-4
  • accession: GCA_020089455
  • assembly level: scaffold
  • database: ncbi
  • NCBI tax ID: 1607837

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
flagellatedno97.921no
gram-positiveyes96.45no
anaerobicno90.654no
aerobicno96.13yes
halophileyes95.214no
spore-formingno95.355no
motileno96.076no
thermophileno99.736yes
glucose-utilyes87.575no
glucose-fermentyes91.273no

External links

@ref: 21008

culture collection no.: DSM 27694

straininfo link

  • @ref: 88107
  • straininfo: 399844

Reference

@idauthorstitledoi/urlcatalogue
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
21008Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-27694Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 27694)
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
68371Automatically annotated from API 50CH acid
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/
88107Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID399844.1