General

@ref: 20971

BacDive-ID: 24082

DSM-Number: 26526

keywords: 16S sequence, Archaea, thermophilic

description: Haloferax chudinovii RS75 is a thermophilic archaeon that was isolated from monolites of mottled sylvinite from Permian potassium salt deposits.

NCBI tax id

• NCBI tax id: 1109010
• Matching level: species

strain history

• @ref: 20971
• history: <- B. Kuznetsov, RAS, Moscow, Centre Bioeng., RS75 <- A.I. Saralov, IEGM

doi: 10.13145/bacdive24082.20230509.8.1

Name and taxonomic classification

LPSN

• @ref: 20215
• description: domain/archaea
• keyword: phylum/methanobacteriota
• domain: Archaea
• phylum: Methanobacteriota
• class: Halobacteria
• order: Haloferacales
• family: Haloferacaceae
• genus: Haloferax
• species: Haloferax chudinovii
• full scientific name: Haloferax chudinovii Saralov et al. 2014

@ref: 20971

domain: Archaea

phylum: Euryarchaeota

class: Halobacteria

order: Halobacteriales

family: Haloferacaceae

genus: Haloferax

species: Haloferax chudinovii

full scientific name: Haloferax chudinovii Saralov et al. 2014

strain designation: RS75

type strain: yes

Culture and growth conditions

culture medium

• @ref: 20971
• name: HALOFERAX MEDIUM RS75 (DSMZ Medium 1481)
• growth: yes
• link: https://mediadive.dsmz.de/medium/1481
• composition: Name: HALOFERAX MEDIUM RS75 (DSMZ Medium 1481) Composition: NaCl 160.0 g/l MgCl2 x 6 H2O 50.0 g/l Agar 20.0 g/l K2SO4 5.0 g/l Na pyruvate 2.0 g/l Yeast extract 1.0 g/l Glucose 0.5 g/l Tryptone 0.5 g/l Na acetate x 3 H2O 0.5 g/l Na malate 0.5 g/l CaCl2 x 2 H2O 0.1 g/l Distilled water

culture temp

• @ref: 20971
• growth: positive
• type: growth
• temperature: 40
• range: thermophilic

Isolation, sampling and environmental information

isolation

• @ref: 20971
• sample type: monolites of mottled sylvinite from Permian potassium salt deposits
• geographic location: foothills of the Pre-Ural depression, Berezniki–Solikams, Upper Kama salt mine, Verkhnekamsk deposit
• country: Romania
• origin.country: ROU
• continent: Europe

isolation source categories

Safety information

risk assessment

• @ref: 20971
• biosafety level: 1
• biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

• @ref: 20971
• description: Haloferax chudinovii strain RS75 16S ribosomal RNA gene, partial sequence
• accession: JX669135
• length: 1425
• database: ena
• NCBI tax ID: 1109010

GC content

• @ref: 20971
• GC-content: 65.0
• method: thermal denaturation, midpoint method (Tm)

External links

@ref: 20971

culture collection no.: DSM 26526, VKPM B-11279

straininfo link

• @ref: 88047
• straininfo: 407174

literature

• topic: Phylogeny
• Pubmed-ID: 23525908
• title: Haloferax chudinovii sp. nov., a halophilic archaeon from Permian potassium salt deposits.
• authors: Saralov AI, Baslerov RV, Kuznetsov BB
• journal: Extremophiles
• DOI: 10.1007/s00792-013-0534-8
• year: 2013
• mesh: Archaeal Proteins/analysis, Base Composition, Carbohydrates/analysis, DNA, Archaeal/chemistry, Geologic Sediments/*microbiology, Haloferax/chemistry/cytology/genetics/*isolation & purification, Lipids/analysis, Phylogeny, Potassium, Siberia
• topic2: Enzymology

Reference