Strain identifier

BacDive ID: 24081

Type strain: Yes

Species: Haloferax mucosum

Strain Designation: PA12

Strain history: M. Allen PA12.

NCBI tax ID(s): 662479 (strain), 403181 (species)

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For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 20970

BacDive-ID: 24081

DSM-Number: 27191

keywords: genome sequence, 16S sequence, Archaea, aerobe, mesophilic, Gram-negative, rod-shaped

description: Haloferax mucosum PA12 is an aerobe, mesophilic, Gram-negative archaeon that was isolated from pustular microbial mat from Hemelin Pool.

NCBI tax id

NCBI tax idMatching level
662479strain
403181species

strain history

@refhistory
20970<- JCM <- M. A. Allen, University of New South Wales; PA12
67770M. Allen PA12.

doi: 10.13145/bacdive24081.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/archaea
  • keyword: phylum/methanobacteriota
  • domain: Archaea
  • phylum: Methanobacteriota
  • class: Halobacteria
  • order: Haloferacales
  • family: Haloferacaceae
  • genus: Haloferax
  • species: Haloferax mucosum
  • full scientific name: Haloferax mucosum Allen et al. 2008

@ref: 20970

domain: Archaea

phylum: Euryarchaeota

class: Halobacteria

order: Halobacteriales

family: Haloferacaceae

genus: Haloferax

species: Haloferax mucosum

full scientific name: Haloferax mucosum Allen et al. 2008

strain designation: PA12

type strain: yes

Morphology

cell morphology

  • @ref: 32347
  • gram stain: negative
  • cell shape: rod-shaped
  • motility: no

pigmentation

  • @ref: 32347
  • production: yes

Culture and growth conditions

culture medium

  • @ref: 20970
  • name: HALOBACTERIA MEDIUM (DSMZ Medium 372)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/372
  • composition: Name: HALOBACTERIA MEDIUM (DSMZ Medium 372) Composition: NaCl 200.0 g/l MgSO4 x 7 H2O 20.0 g/l Agar 20.0 g/l Yeast extract 5.0 g/l Casamino acids 5.0 g/l Na3-citrate 3.0 g/l KCl 2.0 g/l Na glutamate 1.0 g/l FeCl2 x 4 H2O 0.036 g/l MnCl2 x 4 H2O 0.00036 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
20970positivegrowth37mesophilic
32347positivegrowth23-55
32347positiveoptimum47.5thermophilic
67770positivegrowth37mesophilic

culture pH

@refabilitytypepHPH range
32347positivegrowth06-10alkaliphile
32347positiveoptimum7.4

Physiology and metabolism

oxygen tolerance

  • @ref: 32347
  • oxygen tolerance: aerobe

halophily

@refsaltgrowthtested relationconcentration
32347NaClpositivegrowth9.86-29.58 %
32347NaClpositiveoptimum17.4 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3234717234glucose+carbon source
3234717754glycerol+carbon source
3234717306maltose+carbon source
3234717992sucrose+carbon source
3234727082trehalose+carbon source

metabolite production

  • @ref: 32347
  • Chebi-ID: 35581
  • metabolite: indole
  • production: yes

metabolite tests

  • @ref: 32347
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: +

enzymes

@refvalueactivityec
32347catalase+1.11.1.6
32347gelatinase+

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
20970pustular microbial mat from Hemelin PoolShark BayAustraliaAUSAustralia and Oceania
67770Pustular microbial mat from Hemelin PoolShark Bay, Western AustraliaAustraliaAUSAustralia and Oceania

isolation source categories

Cat1Cat2Cat3
#Engineered#Built environment#Water reservoir (Aquarium/pool)
#Environmental#Microbial community#Microbial mat

taxonmaps

  • @ref: 69479
  • File name: preview.99_187153.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_17828;96_68792;97_89434;98_121704;99_187153&stattab=map
  • Last taxonomy: Haloferax
  • 16S sequence: DQ860980
  • Sequence Identity:
  • Total samples: 5
  • soil counts: 2
  • aquatic counts: 3

Safety information

risk assessment

  • @ref: 20970
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 20970
  • description: Haloferax mucosum strain PA12 16S ribosomal RNA gene, partial sequence
  • accession: DQ860980
  • length: 1362
  • database: ena
  • NCBI tax ID: 662479

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Haloferax mucosum PA122508501020draftimg662479
66792Haloferax mucosum PA122529293244draftimg662479
67770Haloferax mucosum ATCC BAA-1512GCA_000337815contigncbi662479

GC content

@refGC-contentmethod
2097061.4high performance liquid chromatography (HPLC)
3234760.8

External links

@ref: 20970

culture collection no.: DSM 27191, ATCC BAA 1512, JCM 14792, UNSW 104200, CGMCC 1.8488

straininfo link

  • @ref: 88046
  • straininfo: 343799

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny18398172Haloferax elongans sp. nov. and Haloferax mucosum sp. nov., isolated from microbial mats from Hamelin Pool, Shark Bay, Australia.Allen MA, Goh F, Leuko S, Echigo A, Mizuki T, Usami R, Kamekura M, Neilan BA, Burns BPInt J Syst Evol Microbiol10.1099/ijs.0.65360-02008Base Composition, DNA, Bacterial/chemistry/genetics, Genes, Bacterial, Haloferax/*classification/genetics/*isolation & purification/metabolism, Lipids/analysis, Molecular Sequence Data, Phenotype, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Seawater/microbiology, Sequence Homology, Nucleic Acid, Species Specificity, Western AustraliaGenetics
Cultivation31525414Physicochemical characterization and emulsifying properties of a novel exopolysaccharide produced by haloarchaeon Haloferax mucosum.Lopez-Ortega MA, Rodriguez-Hernandez AI, Camacho-Ruiz RM, Cordova J, Lopez-Cuellar MDR, Chavarria-Hernandez N, Gonzalez-Garcia YInt J Biol Macromol10.1016/j.ijbiomac.2019.09.0872019Biomass, Biopolymers/biosynthesis/chemistry, Culture Media/chemistry, Emulsions/*chemistry, Haloferax/growth & development/*metabolism, Hydrogen-Ion Concentration, Molecular Weight, Polysaccharides, Bacterial/*biosynthesis/*chemistry, Rheology, Temperature, ViscosityMetabolism

Reference

@idauthorstitledoi/urlcatalogueID_cross_referencepubmed
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
20970Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-27191Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 27191)
32347Barberan A, Caceres Velazquez H, Jones S, Fierer N.Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information10.1128/mSphere.00237-172857828776041
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/
88046Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID343799.1