Strain identifier
BacDive ID: 24080
Type strain: 
Species: Haloferax elongans
Strain Designation: SA5
Strain history: <- JCM <- M. A. Allen, University of New South Wales; SA5
NCBI tax ID(s): 1230453 (strain), 403191 (species)
version 8.1 (current version)
General
@ref: 20969
BacDive-ID: 24080
DSM-Number: 27209
keywords: 16S sequence, Archaea, aerobe, mesophilic, Gram-negative, rod-shaped
description: Haloferax elongans SA5 is an aerobe, mesophilic, Gram-negative archaeon that was isolated from microbial mats from Hamelin Pool.
NCBI tax id
| NCBI tax id | Matching level |
|---|---|
| 403191 | species |
| 1230453 | strain |
strain history
- @ref: 20969
- history: <- JCM <- M. A. Allen, University of New South Wales; SA5
doi: 10.13145/bacdive24080.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/archaea
- keyword: phylum/methanobacteriota
- domain: Archaea
- phylum: Methanobacteriota
- class: Halobacteria
- order: Haloferacales
- family: Haloferacaceae
- genus: Haloferax
- species: Haloferax elongans
- full scientific name: Haloferax elongans Allen et al. 2008
@ref: 20969
domain: Archaea
phylum: Euryarchaeota
class: Halobacteria
order: Halobacteriales
family: Haloferacaceae
genus: Haloferax
species: Haloferax elongans
full scientific name: Haloferax elongans Allen et al. 2008
strain designation: SA5
type strain: yes
Morphology
cell morphology
- @ref: 32347
- gram stain: negative
- cell length: 12 µm
- cell shape: rod-shaped
pigmentation
- @ref: 32347
- production: yes
Culture and growth conditions
culture medium
- @ref: 20969
- name: HALOBACTERIA MEDIUM (DSMZ Medium 372)
- growth: yes
- link: https://mediadive.dsmz.de/medium/372
- composition: Name: HALOBACTERIA MEDIUM (DSMZ Medium 372) Composition: NaCl 200.0 g/l MgSO4 x 7 H2O 20.0 g/l Agar 20.0 g/l Yeast extract 5.0 g/l Casamino acids 5.0 g/l Na3-citrate 3.0 g/l KCl 2.0 g/l Na glutamate 1.0 g/l FeCl2 x 4 H2O 0.036 g/l MnCl2 x 4 H2O 0.00036 g/l Distilled water
culture temp
| @ref | growth | type | temperature | range |
|---|---|---|---|---|
| 20969 | positive | growth | 37 | mesophilic |
| 32347 | positive | growth | 30-55 | |
| 32347 | positive | optimum | 53 | thermophilic |
culture pH
| @ref | ability | type | pH | PH range |
|---|---|---|---|---|
| 32347 | positive | growth | 07-09 | alkaliphile |
| 32347 | positive | optimum | 7.4 |
Physiology and metabolism
oxygen tolerance
- @ref: 32347
- oxygen tolerance: aerobe
halophily
| @ref | salt | growth | tested relation | concentration |
|---|---|---|---|---|
| 32347 | NaCl | positive | growth | 9.86-29.58 % |
| 32347 | NaCl | positive | optimum | 17.4 % |
metabolite utilization
| @ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
|---|---|---|---|---|
| 32347 | 17234 | glucose | + | carbon source |
| 32347 | 17754 | glycerol | + | carbon source |
| 32347 | 17306 | maltose | + | carbon source |
| 32347 | 17992 | sucrose | + | carbon source |
| 32347 | 27082 | trehalose | + | carbon source |
metabolite production
- @ref: 32347
- Chebi-ID: 35581
- metabolite: indole
- production: yes
metabolite tests
- @ref: 32347
- Chebi-ID: 35581
- metabolite: indole
- indole test: +
enzymes
| @ref | value | activity | ec |
|---|---|---|---|
| 32347 | catalase | + | 1.11.1.6 |
| 32347 | gelatinase | + | |
| 32347 | cytochrome oxidase | + | 1.9.3.1 |
Isolation, sampling and environmental information
isolation
- @ref: 20969
- sample type: microbial mats from Hamelin Pool
- geographic location: Shark Bay
- country: Australia
- origin.country: AUS
- continent: Australia and Oceania
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Environmental | #Aquatic | #Marine |
| #Environmental | #Microbial community | #Microbial mat |
Safety information
risk assessment
- @ref: 20969
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 20969
- description: Haloferax elongans strain SA5 16S ribosomal RNA gene, partial sequence
- accession: DQ860977
- length: 1379
- database: ena
- NCBI tax ID: 1230453
GC content
| @ref | GC-content | method |
|---|---|---|
| 20969 | 61.4 | high performance liquid chromatography (HPLC) |
| 32347 | 61.4 |
External links
@ref: 20969
culture collection no.: DSM 27209, ATCC BAA 1513, JCM 14791, UNSW 104100
straininfo link
- @ref: 88045
- straininfo: 343798
literature
- topic: Phylogeny
- Pubmed-ID: 18398172
- title: Haloferax elongans sp. nov. and Haloferax mucosum sp. nov., isolated from microbial mats from Hamelin Pool, Shark Bay, Australia.
- authors: Allen MA, Goh F, Leuko S, Echigo A, Mizuki T, Usami R, Kamekura M, Neilan BA, Burns BP
- journal: Int J Syst Evol Microbiol
- DOI: 10.1099/ijs.0.65360-0
- year: 2008
- mesh: Base Composition, DNA, Bacterial/chemistry/genetics, Genes, Bacterial, Haloferax/*classification/genetics/*isolation & purification/metabolism, Lipids/analysis, Molecular Sequence Data, Phenotype, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Seawater/microbiology, Sequence Homology, Nucleic Acid, Species Specificity, Western Australia
- topic2: Genetics
Reference
| @id | authors | title | doi/url | catalogue | ID_cross_reference | pubmed |
|---|---|---|---|---|---|---|
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 | |||
| 20969 | Curators of the DSMZ | https://www.dsmz.de/collection/catalogue/details/culture/DSM-27209 | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 27209) | |||
| 32347 | Barberan A, Caceres Velazquez H, Jones S, Fierer N. | Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information | 10.1128/mSphere.00237-17 | 28578 | 28776041 | |
| 88045 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | StrainInfo: A central database for resolving microbial strain identifiers | 10.60712/SI-ID343798.1 |