Strain identifier

BacDive ID: 23876

Type strain: Yes

Species: Corynebacterium frankenforstense

Strain Designation: ST18

Strain history: CIP <- 2014, DSMZ <- A. Lipski, Bonn Univ., Bonn, Germany: strain ST18 <- SC Schulz, Bonn Univ., Bonn, Germany

NCBI tax ID(s): 1437875 (strain), 1230998 (species)

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General

@ref: 20516

BacDive-ID: 23876

DSM-Number: 45800

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, Gram-positive, rod-shaped, animal pathogen

description: Corynebacterium frankenforstense ST18 is an aerobe, mesophilic, Gram-positive animal pathogen that was isolated from bulk milk tank of a dairy farm.

NCBI tax id

NCBI tax idMatching level
1230998species
1437875strain

strain history

@refhistory
20516<- A. Lipski, Universität Bonn; ST18 <- S. C. Schulz, Universität Bonn
67770CCUG 63371 <-- A. Lipski; Univ. Bonn, Germany; ST18 <-- S. C. Schulz.
119446CIP <- 2014, DSMZ <- A. Lipski, Bonn Univ., Bonn, Germany: strain ST18 <- SC Schulz, Bonn Univ., Bonn, Germany

doi: 10.13145/bacdive23876.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Mycobacteriales
  • family: Corynebacteriaceae
  • genus: Corynebacterium
  • species: Corynebacterium frankenforstense
  • full scientific name: Corynebacterium frankenforstense Wiertz et al. 2013

@ref: 20516

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Corynebacteriaceae

genus: Corynebacterium

species: Corynebacterium frankenforstense

full scientific name: Corynebacterium frankenforstense Wiertz et al. 2013 emend. Nouioui et al. 2018

strain designation: ST18

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotilityconfidence
31049positive1.25 µm0.75 µmrod-shapedno
69480no92.76
69480positive100
119446positiverod-shapedno

colony morphology

  • @ref: 62973
  • incubation period: 7 days

pigmentation

  • @ref: 31049
  • production: yes

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
41936MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
20516TRYPTICASE SOY BROTH AGAR+BLOOD (DSMZ Medium 535a)yesName: TRYPTICASE SOY BROTH AGAR+BLOOD (DSMZ Medium 535a) Composition: None 50.0 g/l Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled waterhttps://mediadive.dsmz.de/medium/535a
119446CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
20516positivegrowth28mesophilic
31049positivegrowth30-42
41936positivegrowth30mesophilic
62973positivegrowth37mesophilic
67770positivegrowth28mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
31049aerobe
62973microaerophile

spore formation

@refspore formationconfidence
31049no
69480no99.998

observation

  • @ref: 67770
  • observation: quinones: MK-8(H2)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3104922599arabinose+carbon source
3104918403L-arabitol+carbon source
3104928757fructose+carbon source
3104917234glucose+carbon source
3104937684mannose+carbon source
3104933942ribose+carbon source
3104917992sucrose+carbon source
3104927082trehalose+carbon source

enzymes

@refvalueactivityec
31049catalase+1.11.1.6
31049pyrazinamidase+3.5.1.B15

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentsampling date
20516bulk milk tank of a dairy farmBonnGermanyDEUEurope
62973Raw cow milkGermanyDEUEurope2011-03-01
67770Bulk milk tank of a dairy farmGermanyDEUEurope
119446Bulk milk tank of a dairy farmBonnGermanyDEUEurope

isolation source categories

Cat1Cat2Cat3
#Engineered#Built environment#Container (Reservoir)
#Engineered#Food production#Dairy product
#Engineered#Industrial#Engineered product

taxonmaps

  • @ref: 69479
  • File name: preview.99_5150.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_10;96_2505;97_3059;98_3839;99_5150&stattab=map
  • Last taxonomy: Corynebacterium frankenforstense subclade
  • 16S sequence: HE983827
  • Sequence Identity:
  • Total samples: 3537
  • soil counts: 34
  • aquatic counts: 44
  • animal counts: 3445
  • plant counts: 14

Safety information

risk assessment

@refpathogenicity animalbiosafety levelbiosafety level comment
20516yes, in single cases1Risk group (German classification)
1194462Risk group (French classification)

Sequence information

16S sequences

  • @ref: 20516
  • description: Corynebacterium frankenforstense partial 16S rRNA gene, type strain ST18T
  • accession: HE983827
  • length: 1428
  • database: ena
  • NCBI tax ID: 1230998

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Corynebacterium frankenforstense DSM 45800 ST18GCA_001941485completencbi1437875
66792Corynebacterium frankenforstense DSM 458001437875.4completepatric1437875

GC content

  • @ref: 67770
  • GC-content: 71.5
  • method: genome sequence analysis

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno88no
flagellatedno98.031yes
gram-positiveyes92.759yes
anaerobicno98.57no
halophileyes82.776no
spore-formingno94.084no
glucose-utilyes85.699no
aerobicno62.388no
thermophileno90.804yes
motileno94.808no
glucose-fermentyes66.864no

External links

@ref: 20516

culture collection no.: DSM 45800, CCUG 63371, CIP 110785, JCM 32210

straininfo link

  • @ref: 87888
  • straininfo: 397306

literature

  • topic: Phylogeny
  • Pubmed-ID: 23907219
  • title: Corynebacterium frankenforstense sp. nov. and Corynebacterium lactis sp. nov., isolated from raw cow milk.
  • authors: Wiertz R, Schulz SC, Muller U, Kampfer P, Lipski A
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.050757-0
  • year: 2013
  • mesh: Animals, Bacterial Typing Techniques, Cattle, Corynebacterium/*classification/genetics/isolation & purification, DNA, Bacterial/genetics, Fatty Acids/chemistry, *Food Microbiology, Milk/*microbiology, Molecular Sequence Data, Nucleic Acid Hybridization, Peptidoglycan/chemistry, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Vitamin K 2/analogs & derivatives/chemistry
  • topic2: Biotechnology

Reference

@idauthorstitledoi/urlcatalogueID_cross_referencepubmedjournal
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
20516Curators of the DSMZhttps://www.dsmz.de/collection/catalogue/details/culture/DSM-45800Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 45800)
31049Barberan A, Caceres Velazquez H, Jones S, Fierer N.Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information10.1128/mSphere.00237-172737928776041
41936Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/30597
62973Curators of the CCUGhttps://www.ccug.se/strain?id=63371Culture Collection University of Gothenburg (CCUG) (CCUG 63371)
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar SchomburgBRENDA, the ELIXIR core data resource in 2021: new developments and updates10.1093/nar/gkaa102533211880Nucleic Acids Res. 49: D498-D508 2020
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.MicrobeAtlas 1.0 betahttps://microbeatlas.org/
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg OvermannPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)https://diaspora-project.de/progress.html#genomes
87888Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.StrainInfo: A central database for resolving microbial strain identifiers10.60712/SI-ID397306.1
119446Curators of the CIPhttps://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20110785Collection of Institut Pasteur (CIP 110785)