Strain identifier

BacDive ID: 2373

Type strain: Yes

Species: Cellulomonas aerilata

Strain Designation: 5420S-23

Strain history: CIP <- 2010, KACC <- H.-Y. Weon: strain 5420S-23

NCBI tax ID(s): 515326 (species)

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General

@ref: 7635

BacDive-ID: 2373

DSM-Number: 18649

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, Gram-positive, motile, ovoid-shaped

description: Cellulomonas aerilata 5420S-23 is an aerobe, mesophilic, Gram-positive bacterium that was isolated from air sample.

NCBI tax id

  • NCBI tax id: 515326
  • Matching level: species

strain history

@refhistory
403382010, KACC
7635<- S.-W. Kwon, KACC <- H.-Y. Weon; 5420S-23
67770KACC 20692 <-- H.-Y. Weon 5420S-23.
116058CIP <- 2010, KACC <- H.-Y. Weon: strain 5420S-23

doi: 10.13145/bacdive2373.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Micrococcales
  • family: Cellulomonadaceae
  • genus: Cellulomonas
  • species: Cellulomonas aerilata
  • full scientific name: Cellulomonas aerilata Lee et al. 2008

@ref: 7635

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Cellulomonadaceae

genus: Cellulomonas

species: Cellulomonas aerilata

full scientific name: Cellulomonas aerilata Lee et al. 2008

strain designation: 5420S-23

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotilityconfidence
31209positive1.3 µm0.9 µmovoid-shapedyes
69480positive100
116058positiverod-shapedno

pigmentation

  • @ref: 31209
  • production: yes

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
7635R2A MEDIUM (DSMZ Medium 830)yeshttps://mediadive.dsmz.de/medium/830Name: R2A MEDIUM (DSMZ Medium 830) Composition: Agar 15.0 g/l Casamino acids 0.5 g/l Starch 0.5 g/l Glucose 0.5 g/l Proteose peptone 0.5 g/l Yeast extract 0.5 g/l K2HPO4 0.3 g/l Na-pyruvate 0.3 g/l MgSO4 x 7 H2O 0.05 g/l Distilled water
40338MEDIUM 566- Reasoner's 2A agar for Flavobacterium micromatiyesDistilled water make up to (1000.000 ml);R2A agar (18.200 g)
116058CIP Medium 566yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=566

culture temp

@refgrowthtypetemperaturerange
18613positiveoptimum28mesophilic
7635positivegrowth28mesophilic
31209positivegrowth05-35
31209positiveoptimum28mesophilic
40338positivegrowth30mesophilic
67770positivegrowth28mesophilic

culture pH

@refabilitytypepHPH range
31209positivegrowth07-09alkaliphile
31209positiveoptimum07-08

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
31209aerobe
116058obligate aerobe

spore formation

@refspore formationconfidence
31209no
69480no99.913

halophily

@refsaltgrowthtested relationconcentration
31209NaClpositivegrowth0-1 %
31209NaClpositiveoptimum0-1 %

observation

@refobservation
31209aggregates in chains
67770quinones: MK-9(H4)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3120929864mannitol+carbon source
31209506227N-acetylglucosamine+carbon source
312094853esculin+hydrolysis
11605817632nitrate-reduction
11605816301nitrite-reduction
6837917632nitrate-reduction
683794853esculin+hydrolysis
6837916199urea-hydrolysis
683795291gelatin-hydrolysis
6837917634D-glucose-fermentation
6837916988D-ribose-fermentation
6837965327D-xylose-fermentation
6837916899D-mannitol-fermentation
6837917306maltose-fermentation
6837917716lactose-fermentation
6837917992sucrose-fermentation
6837928087glycogen-fermentation

metabolite production

  • @ref: 116058
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
31209catalase+1.11.1.6
31209gelatinase+
31209urease+3.5.1.5
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382beta-glucosidase+3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase-
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase+3.1.3.1
68379gelatinase-
68379urease-3.5.1.5
68379beta-glucosidase+3.2.1.21
68379N-acetyl-beta-glucosaminidase+3.2.1.52
68379alpha-glucosidase+3.2.1.20
68379beta-galactosidase-3.2.1.23
68379beta-glucuronidase-3.2.1.31
68379alkaline phosphatase+3.1.3.1
68379pyrrolidonyl arylamidase-3.4.19.3
68379pyrazinamidase-3.5.1.B15
116058oxidase-
116058catalase+1.11.1.6
116058urease-3.5.1.5

API coryne

@refNITPYZPYRAPALbeta GURbeta GALalpha GLUbeta NAGESCUREGELGLURIBXYLMANMALLACSACGLYG
18613---+--+++----------

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
18613+++-+----+----+++--

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
7635air sampleSuwon regionRepublic of KoreaKORAsia
67770AirSuwon regionRepublic of KoreaKORAsia
116058Environment, Air sampleSuwonRepublic of KoreaKORAsia

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Air

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
76351Risk group (German classification)
1160581Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
7635Cellulomonas aerilata strain 5420S-23 16S ribosomal RNA gene, partial sequenceEU5609791387ena515326
67770Cellulomonas aerilata gene for 16S ribosomal RNA, partial sequence, strain: JCM 16376AB9105211474ena515326

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Cellulomonas aerilata NBRC 106308GCA_007991755contigncbi515326
66792Cellulomonas aerilata strain NBRC 106308515326.3wgspatric515326

GC content

@refGC-contentmethod
763574.0
3120974
6777074high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes86no
gram-positiveyes87.882yes
anaerobicno99.174no
halophileno95.5no
spore-formingno92.781yes
glucose-utilyes84.065no
aerobicyes93.457yes
thermophileno99.084yes
flagellatedno89.322no
motileyes79.656no
glucose-fermentno83.224yes

External links

@ref: 7635

culture collection no.: DSM 18649, KACC 20692, JCM 16376, NBRC 106308, CIP 110191

straininfo link

  • @ref: 71928
  • straininfo: 360267

literature

  • topic: Phylogeny
  • Pubmed-ID: 19060084
  • title: Cellulomonas aerilata sp. nov., isolated from an air sample.
  • authors: Lee CM, Weon HY, Hong SB, Jeon YA, Schumann P, Kroppenstedt RM, Kwon SW, Stackebrandt E
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.2008/002253-0
  • year: 2008
  • mesh: *Air Microbiology, Cellulomonas/chemistry/*classification/genetics/*physiology, Fatty Acids/analysis, Korea, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics, Species Specificity
  • topic2: Genetics

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmedjournal
7635Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 18649)https://www.dsmz.de/collection/catalogue/details/culture/DSM-18649
18613Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM18649.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
31209Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2753628776041
40338Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/8023
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updates33211880Nucleic Acids Res. 49: D498-D508 2020
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68379Automatically annotated from API Coryne
68382Automatically annotated from API zym
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
71928Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID360267.1StrainInfo: A central database for resolving microbial strain identifiers
116058Curators of the CIPCollection of Institut Pasteur (CIP 110191)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20110191