Strain identifier

BacDive ID: 2352

Type strain: Yes

Species: Cellulomonas fimi

Strain Designation: 133

Culture col. no.: DSM 20113, ATCC 484, NCIB 8980, NCTC 7547, CCUG 24087

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General

@ref: 8538

BacDive-ID: 2352

DSM-Number: 20113

keywords: rod-shaped, motile, gram-positive, mesophilic, aerobe, Bacteria, 16S sequence, genome sequence

description: Cellulomonas fimi 133 is an aerobe, mesophilic, gram-positive bacterium that was isolated from soil.

strain history: <- ATCC <- N.R. Smith, 133

doi: 10.13145/bacdive2352.20201210.5

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinobacteria
  • domain: Bacteria
  • phylum: Actinobacteria
  • class: Actinobacteria
  • order: Micrococcales
  • family: Cellulomonadaceae
  • genus: Cellulomonas
  • species: Cellulomonas fimi
  • full scientific name: Cellulomonas fimi (McBeth and Scales 1913) Bergey et al. 1923 (Approved Lists 1980)
  • synonyms

    • @ref: 20215
    • synonym: Bacterium fimi

@ref: 8538

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Cellulomonadaceae

genus: Cellulomonas

species: Cellulomonas fimi

full scientific name: Cellulomonas fimi (McBeth and Scales 1913) Bergey et al. 1923 emend. Nouioui et al. 2018

strain designation: 133

type strain: yes

Morphology

cell morphology

  • @ref: 43361
  • gram stain: positive
  • cell shape: rod-shaped
  • motility: yes

colony morphology

@refcolony colorincubation periodmedium usedcolony size
18304Colorless10-14 daysISP 2
18304Colorless10-14 daysISP 3
43361white or yellowyeast extract glucose agar5 mm

multicellular morphology

@refforms multicellular complexmedium name
18304noISP 2
18304noISP 3

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
18304ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
18304ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
8538CORYNEBACTERIUM AGAR (DSMZ Medium 53)yeshttps://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium53.pdf
40361MEDIUM 3 - Columbia agaryesColumbia agar (39.000 g);distilled water (1000.000 ml)
43361yeast extract glucose agaryespeptone 1%, yeast extract 0.5%, glucose 0.5%, agar 1%

culture temp

@refgrowthtypetemperaturerange
18304positiveoptimum28mesophilic
8538positivegrowth30mesophilic
40361positivegrowth30mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 43361
  • oxygen tolerance: aerobe

halophily

  • @ref: 18304
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 0 %

murein

  • @ref: 8538
  • murein short key: A21.05
  • type: A4ß L-Orn-D-Glu

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
4336116651(S)-lactate+assimilation
4336130089acetate-assimilation
4336162968cellulose+degradation
4336124265gluconate-assimilation
4336117632nitrate+reduction
4336116634raffinose-assimilation
4336133942ribose-assimilation

API coryne

@refNITPYZPYRAPALbeta GURbeta GALalpha GLUbeta NAGESCUREGELGLURIBXYLMANMALLACSACGLYG
18304--+-+++--++++-+++--

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
18304--+-+++--++++-+++--

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGEL
18304+----------

Isolation, sampling and environmental information

isolation

@refsample type
8538soil
47916Soil

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Terrestrial
  • Cat3: #Soil

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
183041Hazard group
85381Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218C.fimi DSM 20113 16S rDNAX794601486ena590998
20218C.fimi 16S rRNA geneX838031452ena590998

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Cellulomonas fimi ATCC 484GCA_000212695completencbi590998
66792Cellulomonas fimi NCTC7547GCA_900637695completencbi1708
66792Cellulomonas fimi SBGCA_012952065contigncbi1708
66792Cellulomonas fimi W7543GCA_013004555contigncbi1708
66792Cellulomonas fimi ATCC 484590998.5completepatric590998
66792Cellulomonas fimi strain NCTC75471708.3completepatric1708
66792Cellulomonas fimi NRS 133, ATCC 4842505679089completeimg590998

GC content

@refGC-contentmethod
853871.7
4336171.3thermal denaturation, midpoint method (Tm)

External links

@ref: 8538

culture collection no.: DSM 20113, ATCC 484, NCIB 8980, NCTC 7547, CCUG 24087

straininfo link

@refpassport
20218http://www.straininfo.net/strains/33615
20218http://www.straininfo.net/strains/33612
20218http://www.straininfo.net/strains/93831
20218http://www.straininfo.net/strains/33624

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
8538Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 20113)https://www.dsmz.de/collection/catalogue/details/culture/DSM-20113
18304Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM20113.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215D.Gleim, M.Kracht, N.Weiss et. al.http://www.dsmz.de/bacterial-diversity/prokaryotic-nomenclature-up-to-date.htmlProkaryotic Nomenclature Up-to-date - compilation of all names of Bacteria and Archaea, validly published according to the Bacteriological Code since 1. Jan. 1980, and validly published nomenclatural changes since
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
40361Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/13765
43361Erko Stackenbrandt, Otto Kandler10.1099/00207713-29-4-273Taxonomy of the Genus Cellulomonas, Based on Phenotypic Characters and Deoxyribonucleic Acid-Deoxyribonucleic Acid Homology, and Proposal of Seven Neotype StrainsIJSEM 29: 273-282 1979
47916Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 24087)https://www.ccug.se/strain?id=24087
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)