Strain identifier
BacDive ID: 23392
Type strain: 
Species: unclassified bacterium
Strain Designation: NapHS2
Strain history: <- A. Galushko, Universität Konstanz, Fakultät für Biologie, Konstanz, Germany; NapHS2 <- A. Galushko {1997}
NCBI tax ID(s): 1869227 (species)
version 8.1 (current version)
General
@ref: 5361
BacDive-ID: 23392
DSM-Number: 14454
keywords: genome sequence, 16S sequence, anaerobe, mesophilic, Gram-negative
description: unclassified bacterium NapHS2 is an anaerobe, mesophilic, Gram-negative prokaryote that was isolated from black anoxic marine sediment.
NCBI tax id
- NCBI tax id: 1869227
- Matching level: species
strain history
- @ref: 5361
- history: <- A. Galushko, Universität Konstanz, Fakultät für Biologie, Konstanz, Germany; NapHS2 <- A. Galushko {1997}
doi: 10.13145/bacdive23392.20230509.8.1
Name and taxonomic classification
@ref: 5361
phylum: Not assigned to order
class: Not assigned to order
order: Clostridiales
family: Not assigned to family
genus: unclassified
species: unclassified bacterium
full scientific name: unclassified bacterium
strain designation: NapHS2
type strain: no
Morphology
cell morphology
- @ref: 69480
- gram stain: negative
- confidence: 99.999
colony morphology
- @ref: 5361
- incubation period: >14 days
Culture and growth conditions
culture medium
- @ref: 5361
- name: DESULFOBACTERIUM MEDIUM (DSMZ Medium 383)
- growth: yes
- link: https://mediadive.dsmz.de/medium/383
- composition: Name: DESULFOBACTERIUM MEDIUM (DSMZ Medium 383; with strain-specific modifications) Composition: NaCl 20.9372 g/l Na2SO4 2.99103 g/l MgCl2 x 6 H2O 2.99103 g/l Na2CO3 0.997009 g/l KCl 0.498504 g/l Na2S x 9 H2O 0.398804 g/l Naphthalene 0.398804 g/l NH4Cl 0.299103 g/l KH2PO4 0.199402 g/l CaCl2 x 2 H2O 0.149551 g/l HCl 0.00249252 g/l FeCl2 x 4 H2O 0.00149551 g/l Sodium resazurin 0.000498504 g/l NaOH 0.000498504 g/l CoCl2 x 6 H2O 0.000189432 g/l Pyridoxine hydrochloride 9.97009e-05 g/l MnCl2 x 4 H2O 9.97009e-05 g/l ZnCl2 6.97906e-05 g/l Calcium D-(+)-pantothenate 4.98504e-05 g/l Thiamine HCl 4.98504e-05 g/l Nicotinic acid 4.98504e-05 g/l Riboflavin 4.98504e-05 g/l (DL)-alpha-Lipoic acid 4.98504e-05 g/l p-Aminobenzoic acid 4.98504e-05 g/l Na2MoO4 x 2 H2O 3.58923e-05 g/l NiCl2 x 6 H2O 2.39282e-05 g/l Biotin 1.99402e-05 g/l Folic acid 1.99402e-05 g/l H3BO3 5.98205e-06 g/l Na2WO4 x 2 H2O 3.98804e-06 g/l Na2SeO3 x 5 H2O 2.99103e-06 g/l CuCl2 x 2 H2O 1.99402e-06 g/l Vitamin B12 9.97009e-07 g/l 2,2,4,4,6,8,8-Heptamethylnonane
culture temp
- @ref: 5361
- growth: positive
- type: growth
- temperature: 30
- range: mesophilic
Physiology and metabolism
oxygen tolerance
| @ref | oxygen tolerance | confidence |
|---|---|---|
| 5361 | anaerobe | |
| 69480 | anaerobe | 99.138 |
spore formation
| @ref | spore formation | confidence |
|---|---|---|
| 69481 | no | 97 |
| 69480 | no | 99.687 |
Isolation, sampling and environmental information
isolation
- @ref: 5361
- sample type: black anoxic marine sediment
- geographic location: North Sea Coast near Wilhelmshaven, Horumersiel
- country: Germany
- origin.country: DEU
- continent: Europe
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Environmental | #Aquatic | #Marine |
| #Environmental | #Terrestrial | #Sediment |
| #Condition | #Anoxic (anaerobic) |
taxonmaps
- @ref: 69479
- File name: preview.99_3922.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_433;96_2000;97_2408;98_2978;99_3922&stattab=map
- Last taxonomy: Desulfobacteraceae
- 16S sequence: AJ132804
- Sequence Identity:
- Total samples: 50
- soil counts: 2
- aquatic counts: 48
Sequence information
16S sequences
- @ref: 5361
- description: delta proteobacterium NaphS2 16S rRNA gene
- accession: AJ132804
- length: 1477
- database: ena
- NCBI tax ID: 88274
Genome sequences
- @ref: 66792
- description: delta proteobacterium NaphS2
- accession: 88274.4
- assembly level: wgs
- database: patric
- NCBI tax ID: 88274
Genome-based predictions
predictions
| trait | prediction | confidence | training_data |
|---|---|---|---|
| spore-forming | no | 97 | no |
| motile | no | 75.906 | no |
| flagellated | no | 96.14 | no |
| gram-positive | no | 99.172 | no |
| anaerobic | yes | 97.649 | no |
| halophile | no | 62.987 | no |
| spore-forming | no | 91.345 | no |
| thermophile | no | 96.328 | yes |
| glucose-util | yes | 53.348 | no |
| aerobic | no | 94.667 | yes |
| glucose-ferment | no | 85.282 | no |
External links
@ref: 5361
culture collection no.: DSM 14454
Reference
| @id | authors | catalogue | doi/url | title |
|---|---|---|---|---|
| 5361 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 14454) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-14454 | |
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |
| 69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |
| 69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | 10.21203/rs.3.rs-2527258/v1 | Predictions based on the model GenomeNet Sporulation v. 1 |