General

@ref: 4818

BacDive-ID: 23390

DSM-Number: 12783

keywords: anaerobe, mesophilic

description: unclassified bacterium 72Chol is an anaerobe, mesophilic prokaryote that was isolated from freshwater mud.

NCBI tax id

• NCBI tax id: 1869227
• Matching level: species

strain history

• @ref: 4818
• history: <- J. Harder; 72Chol

doi: 10.13145/bacdive23390.20230509.8.1

Name and taxonomic classification

@ref: 4818

phylum: Not assigned to order

class: Not assigned to order

order: Clostridiales

family: Not assigned to family

genus: unclassified

species: unclassified bacterium

full scientific name: unclassified bacterium

strain designation: 72Chol

type strain: no

Morphology

colony morphology

• @ref: 4818
• incubation period: >14 days

Culture and growth conditions

culture medium

• @ref: 4818
• name: ANAEROBIC CHOLESTEROL MEDIUM (DSMZ Medium 858)
• growth: yes
• link: https://mediadive.dsmz.de/medium/858
• composition: Name: ANAEROBIC CHOLESTEROL MEDIUM (DSMZ Medium 858) Composition: NaHCO3 4.2 g/l NaNO3 0.72 g/l KH2PO4 0.5 g/l NH4Cl 0.45 g/l MgSO4 x 7 H2O 0.45 g/l CaCl2 0.09 g/l HCl 0.00625 g/l FeCl2 x 4 H2O 0.00375 g/l NaOH 0.00125 g/l CoCl2 x 6 H2O 0.000475 g/l Riboflavin 0.00025 g/l alpha-lipoic acid 0.00025 g/l p-Aminobenzoic acid 0.00025 g/l Thiamine-HCl x 2 H2O 0.00025 g/l Pantothenic acid 0.00025 g/l Vitamin B12 0.00025 g/l MnCl2 x 4 H2O 0.00025 g/l ZnCl2 0.000175 g/l Nicotine amide 0.000125 g/l Nicotinic acid 0.000125 g/l Folic acid 0.0001 g/l Biotin 0.0001 g/l Na2MoO4 x 2 H2O 9e-05 g/l NiCl2 x 6 H2O 6e-05 g/l Pyridoxamine hydrochloride 5e-05 g/l H3BO3 1.5e-05 g/l Na2WO4 x 2 H2O 1e-05 g/l Na2SeO3 x 5 H2O 7.5e-06 g/l CuCl2 x 2 H2O 5e-06 g/l Distilled water

culture temp

• @ref: 4818
• growth: positive
• type: growth
• temperature: 30
• range: mesophilic

Physiology and metabolism

oxygen tolerance

• @ref: 4818
• oxygen tolerance: anaerobe

Isolation, sampling and environmental information

isolation

• @ref: 4818
• sample type: freshwater mud
• geographic location: near Bremen
• country: Germany
• origin.country: DEU
• continent: Europe

isolation source categories

External links

@ref: 4818

culture collection no.: DSM 12783

Reference