General

@ref: 19212

BacDive-ID: 23218

DSM-Number: 45853

keywords: 16S sequence, Bacteria, mesophilic

description: Pseudonocardia hispaniensis PA3 is a mesophilic bacterium that was isolated from an oil refinery wastewater treatment plant.

NCBI tax id

• NCBI tax id: 904933
• Matching level: species

strain history

• @ref: 19212
• history: <- CECT; CECT 8030 <- G. Cuesta, Univ. Politécnica de Valencia, Spain; PA3

doi: 10.13145/bacdive23218.20230509.8.1

Name and taxonomic classification

LPSN

• @ref: 20215
• description: domain/bacteria
• keyword: phylum/actinomycetota
• domain: Bacteria
• phylum: Actinomycetota
• class: Actinomycetes
• order: Pseudonocardiales
• family: Pseudonocardiaceae
• genus: Pseudonocardia
• species: Pseudonocardia hispaniensis
• full scientific name: Pseudonocardia hispaniensis Cuesta et al. 2013

@ref: 19212

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Pseudonocardiaceae

genus: Pseudonocardia

species: Pseudonocardia hispaniensis

full scientific name: Pseudonocardia hispaniensis Cuesta et al. 2013

strain designation: PA3

type strain: yes

Culture and growth conditions

culture medium

• @ref: 19212
• name: GPHF-MEDIUM (DSMZ Medium 553)
• growth: yes
• link: https://mediadive.dsmz.de/medium/553
• composition: Name: GPHF-MEDIUM (DSMZ Medium 553) Composition: Agar 20.0 g/l Glucose 10.0 g/l Beef extract 5.0 g/l Yeast extract 5.0 g/l Casein peptone 5.0 g/l CaCl2 x 2 H2O 0.74 g/l Distilled water

culture temp

• @ref: 19212
• growth: positive
• type: growth
• temperature: 28
• range: mesophilic

Isolation, sampling and environmental information

isolation

• @ref: 19212
• sample type: an oil refinery wastewater treatment plant
• geographic location: Palos de la Frontera (Huelva)
• country: Spain
• origin.country: ESP
• continent: Europe

isolation source categories

Safety information

risk assessment

• @ref: 19212
• biosafety level: 1
• biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

• @ref: 19212
• description: Pseudonocardia hispaniensis partial 16S rRNA gene, type strain PA3T
• accession: FR695486
• length: 1373
• database: ena
• NCBI tax ID: 904933

GC content

• @ref: 19212
• GC-content: 69.7

External links

@ref: 19212

culture collection no.: DSM 45853, CCM 8391, CECT 8030, KCTC 29108

straininfo link

• @ref: 87446
• straininfo: 387511

literature

• topic: Phylogeny
• Pubmed-ID: 22899021
• title: Pseudonocardia hispaniensis sp. nov., a novel actinomycete isolated from industrial wastewater activated sludge.
• authors: Cuesta G, Soler A, Alonso JL, Ruvira MA, Lucena T, Arahal DR, Goodfellow M
• journal: Antonie Van Leeuwenhoek
• DOI: 10.1007/s10482-012-9792-1
• year: 2012
• mesh: Actinomycetales/*classification/genetics/*isolation & purification, Bacterial Typing Techniques, Cluster Analysis, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Fatty Acids/analysis, *Industrial Waste, Molecular Sequence Data, Nucleic Acid Hybridization, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sewage/*microbiology, Spain, Vitamin K 2/analysis, Waste Water/*microbiology
• topic2: Genetics

Reference