Strain identifier

BacDive ID: 23131

Type strain: Yes

Species: Psychrobacter okhotskensis

Strain Designation: MD17

Strain history: CIP <- 2003, JCM <- 2002, I. Yumoto: strain MD17

NCBI tax ID(s): 212403 (species)

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General

@ref: 18906

BacDive-ID: 23131

DSM-Number: 18357

keywords: 16S sequence, Bacteria, obligate aerobe, psychrophilic, Gram-negative, oval-shaped

description: Psychrobacter okhotskensis MD17 is an obligate aerobe, psychrophilic, Gram-negative bacterium that was isolated from Seawater from the Monbetsu coast of the Okhotsk Sea in Hokkaido.

NCBI tax id

  • NCBI tax id: 212403
  • Matching level: species

strain history

@refhistory
18906<- JCM <- I. Yumoto, Institute for Biological Resources and Function, National Institute of Advanced Industrial Science and Technology, Sapporo, Japan; MD17
67770I. Yumoto MD17.
121406CIP <- 2003, JCM <- 2002, I. Yumoto: strain MD17

doi: 10.13145/bacdive23131.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Pseudomonadales
  • family: Moraxellaceae
  • genus: Psychrobacter
  • species: Psychrobacter okhotskensis
  • full scientific name: Psychrobacter okhotskensis Yumoto et al. 2003

@ref: 18906

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Pseudomonadales

family: Moraxellaceae

genus: Psychrobacter

species: Psychrobacter okhotskensis

full scientific name: Psychrobacter okhotskensis Yumoto et al. 2003

strain designation: MD17

type strain: yes

Morphology

cell morphology

  • @ref: 121406
  • gram stain: negative
  • cell shape: oval-shaped
  • motility: no

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
18906BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)yeshttps://mediadive.dsmz.de/medium/514Name: BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514) Composition: NaCl 19.45 g/l MgCl2 5.9 g/l Bacto peptone 5.0 g/l Na2SO4 3.24 g/l CaCl2 1.8 g/l Yeast extract 1.0 g/l KCl 0.55 g/l NaHCO3 0.16 g/l Fe(III) citrate 0.1 g/l KBr 0.08 g/l SrCl2 0.034 g/l H3BO3 0.022 g/l Na2HPO4 0.008 g/l Na-silicate 0.004 g/l NaF 0.0024 g/l (NH4)NO3 0.0016 g/l Distilled water
33918Marine agar (MA)yesDistilled water make up to (1000.000 ml);Marine agar (55.100 g)
121406CIP Medium 13yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=13
121406CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
18906positivegrowth20psychrophilic
33918positivegrowth20psychrophilic
67770positivegrowth20psychrophilic
121406positivegrowth5-37
121406nogrowth41thermophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 121406
  • oxygen tolerance: obligate aerobe

halophily

  • @ref: 121406
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 0-10 %

observation

  • @ref: 67770
  • observation: quinones: Q-8

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
12140616947citrate-carbon source
1214064853esculin-hydrolysis
12140617632nitrate-reduction
12140616301nitrite-reduction
12140617632nitrate-respiration

antibiotic resistance

  • @ref: 121406
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

  • @ref: 121406
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
12140615688acetoin-
12140617234glucose-

enzymes

@refvalueactivityec
121406oxidase+
121406beta-galactosidase-3.2.1.23
121406alcohol dehydrogenase-1.1.1.1
121406gelatinase-
121406amylase-
121406DNase+
121406caseinase-3.4.21.50
121406catalase+1.11.1.6
121406tween esterase-
121406gamma-glutamyltransferase+2.3.2.2
121406lecithinase-
121406lipase-
121406lysine decarboxylase-4.1.1.18
121406ornithine decarboxylase-4.1.1.17
121406phenylalanine ammonia-lyase+4.3.1.24
121406tryptophan deaminase-
121406urease-3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)+
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase-3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
121406-+++++-----+--------

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
121406-----------------------------------------------------------------------------------+----+-+--------

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinentgeographic location
18906Seawater from the Monbetsu coast of the Okhotsk Sea in HokkaidoJapanJPNAsia
67770Seawater from the Monbetsu coast of the Okhotsk Sea in HokkaidoJapanJPNAsia
121406Environment, Sea water, OkhotskJapanJPNAsiaHokkaido

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Marine
#Environmental#Terrestrial#Coast

taxonmaps

  • @ref: 69479
  • File name: preview.99_279.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_80;96_193;97_209;98_234;99_279&stattab=map
  • Last taxonomy: Psychrobacter
  • 16S sequence: AB094794
  • Sequence Identity:
  • Total samples: 7634
  • soil counts: 925
  • aquatic counts: 3187
  • animal counts: 3350
  • plant counts: 172

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
189061Risk group (German classification)
1214061Risk group (French classification)

Sequence information

16S sequences

  • @ref: 18906
  • description: Psychrobacter okhotskensis gene for 16S ribosomal RNA, partial sequence
  • accession: AB094794
  • length: 1492
  • database: ena
  • NCBI tax ID: 212403

GC content

  • @ref: 67770
  • GC-content: 46.7
  • method: high performance liquid chromatography (HPLC)

External links

@ref: 18906

culture collection no.: DSM 18357, JCM 11840, CIP 108221, NCIMB 13931

straininfo link

  • @ref: 87366
  • straininfo: 128012

literature

  • topic: Phylogeny
  • Pubmed-ID: 14657134
  • title: Psychrobacter okhotskensis sp. nov., a lipase-producing facultative psychrophile isolated from the coast of the Okhotsk Sea.
  • authors: Yumoto I, Hirota K, Sogabe Y, Nodasaka Y, Yokota Y, Hoshino T
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.02686-0
  • year: 2003
  • mesh: Base Composition, Base Sequence, DNA, Bacterial/chemistry, Japan, Molecular Sequence Data, Moraxellaceae/*classification/enzymology/genetics/isolation & purification, *Phylogeny, Seawater/microbiology, Temperature
  • topic2: Genetics

Reference

@idauthorscataloguedoi/urltitle
18906Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 18357)https://www.dsmz.de/collection/catalogue/details/culture/DSM-18357
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
33918Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/5820
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
87366Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID128012.1StrainInfo: A central database for resolving microbial strain identifiers
121406Curators of the CIPCollection of Institut Pasteur (CIP 108221)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20108221