Strain identifier

BacDive ID: 230

Type strain: Yes

Species: Eremococcus coleocola

Strain Designation: M1832/95/2

Strain history: CIP <- 1999, CCUG <- M.D. Collins, IFR, Reading, UK <- G. Foster, Inverness, UK: strain M1832/95/2

NCBI tax ID(s): 1121871 (strain), 88132 (species)

« back to strain detail view
For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 8.1 (current version):
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 4.1:
version 4:
version 3:
version 2.1:
version 2:
version 1:
version 8.1 (current version)

General

@ref: 6062

BacDive-ID: 230

DSM-Number: 15696

keywords: genome sequence, 16S sequence, Bacteria, microaerophile, mesophilic, Gram-positive, coccus-shaped, colony-forming

description: Eremococcus coleocola M1832/95/2 is a microaerophile, mesophilic, Gram-positive bacterium that forms circular colonies and was isolated from equine vaginal discharge, thoroughbred cross horse.

NCBI tax id

NCBI tax idMatching level
1121871strain
88132species

strain history

@refhistory
6062<- CCUG <- M. D. Collins, IFR Reading <- G. Foster
118335CIP <- 1999, CCUG <- M.D. Collins, IFR, Reading, UK <- G. Foster, Inverness, UK: strain M1832/95/2

doi: 10.13145/bacdive230.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Aerococcaceae
  • genus: Eremococcus
  • species: Eremococcus coleocola
  • full scientific name: Eremococcus coleocola Collins et al. 1999

@ref: 6062

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Aerococcaceae

genus: Eremococcus

species: Eremococcus coleocola

full scientific name: Eremococcus coleocola Collins et al. 1999

strain designation: M1832/95/2

type strain: yes

Morphology

cell morphology

@refgram staincell shapemotilityconfidence
23252positivecoccus-shaped
69480no94.385
69480positive100
118335positivecoccus-shapedno

colony morphology

@reftype of hemolysishemolysis abilitycolony colorcolony shapeincubation periodmedium used
23252alpha1shinycircular1 dayblood agar
1183351

multimedia

@refmultimedia contentcaptionintellectual property rights
66793EM_DSM_15696_1.jpgelectron microscopic image© HZI/Manfred Rohde
66793EM_DSM_15696_2.jpgelectron microscopic image© HZI/Manfred Rohde
66793EM_DSM_15696_3.jpgelectron microscopic image© HZI/Manfred Rohde
66793EM_DSM_15696_4.jpgelectron microscopic image© HZI/Manfred Rohde
66793EM_DSM_15696_5.jpgelectron microscopic image© HZI/Manfred Rohde

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
6062TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)yeshttps://mediadive.dsmz.de/medium/92Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water
6062COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
23252blood agaryes
39489MEDIUM 6 - Columbia agar with 10 % horse bloodyesDistilled water make up to (1000.000 ml);Columbia agar (39.000 g);Horseblood (100.000 ml)
118335CIP Medium 6yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=6
118335CIP Medium 85yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=85

culture temp

@refgrowthtypetemperaturerange
6062positivegrowth37mesophilic
23252positivegrowth42thermophilic
23252nogrowth10psychrophilic
39489positivegrowth37mesophilic
118335positivegrowth25-41
118335nogrowth10psychrophilic
118335nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
6062microaerophile
23252facultative anaerobe
118335facultative anaerobe

spore formation

@refspore formationconfidence
69481no100
69480no99.992

halophily

@refsaltgrowthtested relationconcentration
23252NaClpositivegrowth6.5 %
23252NaClnogrowth10 %
118335NaClpositivegrowth6.5 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2325223456cyclodextrin-builds acid from
2325218333D-arabitol-builds acid from
2325216634raffinose-builds acid from
2325216988D-ribose-builds acid from
2325265327D-xylose-builds acid from
2325228087glycogen-builds acid from
2325230849L-arabinose-builds acid from
2325217716lactose-builds acid from
2325217306maltose-builds acid from
2325229864mannitol-builds acid from
232526731melezitose-builds acid from
2325228053melibiose-builds acid from
23252320055methyl beta-D-glucopyranoside-builds acid from
2325227941pullulan-builds acid from
2325230911sorbitol-builds acid from
2325217992sucrose-builds acid from
2325233954tagatose-builds acid from
2325227082trehalose-builds acid from
232524853esculin-hydrolysis
232525291gelatin-hydrolysis
2325228017starch-hydrolysis
2325217632nitrate-reduction
2325217234glucose+builds acid from
23252606565hippurate+hydrolysis
23252506227N-acetylglucosamine+/-builds acid from
68371Potassium 2-ketogluconate-builds acid from
6837124265gluconate+builds acid from
6837118403L-arabitol-builds acid from
6837118333D-arabitol-builds acid from
6837118287L-fucose-builds acid from
6837128847D-fucose-builds acid from
6837116443D-tagatose-builds acid from
6837132528turanose+builds acid from
6837128066gentiobiose-builds acid from
6837117151xylitol-builds acid from
6837128087glycogen+builds acid from
6837128017starch+builds acid from
6837116634raffinose-builds acid from
683716731melezitose-builds acid from
6837115443inulin-builds acid from
6837127082trehalose+builds acid from
6837128053melibiose-builds acid from
6837117716lactose-builds acid from
6837117306maltose+builds acid from
6837117057cellobiose-builds acid from
6837117814salicin-builds acid from
683714853esculin-builds acid from
6837118305arbutin-builds acid from
6837127613amygdalin-builds acid from
6837159640N-acetylglucosamine+builds acid from
6837143943methyl alpha-D-mannoside-builds acid from
6837117924D-sorbitol+builds acid from
6837116899D-mannitol+builds acid from
6837117268myo-inositol-builds acid from
6837116813galactitol-builds acid from
6837162345L-rhamnose-builds acid from
6837117266L-sorbose-builds acid from
6837116024D-mannose+builds acid from
6837115824D-fructose+builds acid from
6837117634D-glucose+builds acid from
6837112936D-galactose-builds acid from
6837174863methyl beta-D-xylopyranoside-builds acid from
6837115963ribitol-builds acid from
6837165328L-xylose-builds acid from
6837165327D-xylose-builds acid from
6837116988D-ribose-builds acid from
6837130849L-arabinose-builds acid from
6837117108D-arabinose-builds acid from
6837117113erythritol-builds acid from
6837117754glycerol-builds acid from
1183354853esculin-hydrolysis
11833517632nitrate-reduction
11833516301nitrite-reduction
6838129016arginine+hydrolysis
6838116988D-ribose-builds acid from
6838116899D-mannitol-builds acid from
6838130911sorbitol-builds acid from
6838117716lactose-builds acid from
6838127082trehalose-builds acid from
6838116634raffinose-builds acid from
6838117992sucrose-builds acid from
6838130849L-arabinose-builds acid from
6838118333D-arabitol-builds acid from
6838140585alpha-cyclodextrin-builds acid from
68381606565hippurate+hydrolysis
6838128087glycogen-builds acid from
6838127941pullulan-builds acid from
6838117306maltose-builds acid from
6838128053melibiose-builds acid from
683816731melezitose-builds acid from
68381320055methyl beta-D-glucopyranoside-builds acid from
6838116443D-tagatose-builds acid from
6838116199urea-hydrolysis

metabolite production

@refChebi-IDmetaboliteproduction
2325215688acetoinno
11833535581indoleno
6838115688acetoinno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-test
2325215688acetoin-
6838115688acetoin-
11833515688acetoin-

enzymes

@refvalueactivityec
23252acid phosphatase-3.1.3.2
23252alkaline phosphatase-3.1.3.1
23252alpha-fucosidase-3.2.1.51
23252alpha-galactosidase-3.2.1.22
23252alpha-mannosidase-3.2.1.24
23252arginine dihydrolase+3.5.3.6
23252beta-galactosidase-3.2.1.23
23252beta-glucosidase-3.2.1.21
23252beta-glucuronidase-3.2.1.31
23252beta-mannosidase-3.2.1.25
23252catalase-1.11.1.6
23252chymotrypsin-3.4.4.5
23252esterase (C 4)+
23252esterase lipase (C 8)+
23252glycyl tryptophan arylamidase-
23252leucine arylamidase-3.4.11.1
23252lipase (C 14)-
23252pyrazinamidase-3.5.1.B15
23252pyroglutamic acid arylamidase+
23252tripeptide aminopeptidase-3.4.11.4
23252trypsin-3.4.21.4
23252urease+/-3.5.1.5
23252valine arylamidase-
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase+3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase-3.1.3.1
68381urease-3.5.1.5
68381beta-mannosidase-3.2.1.25
68381glycyl tryptophan arylamidase-
68381N-acetyl-beta-glucosaminidase-3.2.1.52
68381pyrrolidonyl arylamidase+3.4.19.3
68381beta-galactosidase-3.2.1.23
68381Alanyl-Phenylalanyl-Proline arylamidase-
68381alkaline phosphatase-3.1.3.1
68381alpha-galactosidase-3.2.1.22
68381beta-glucuronidase-3.2.1.31
68381beta-glucosidase-3.2.1.21
68381arginine dihydrolase+3.5.3.6
118335oxidase+
118335beta-galactosidase-3.2.1.23
118335alcohol dehydrogenase+1.1.1.1
118335catalase-1.11.1.6
118335gamma-glutamyltransferase+2.3.2.2
118335lysine decarboxylase-4.1.1.18
118335ornithine decarboxylase-4.1.1.17
118335urease-3.5.1.5

fatty acid profile

  • fatty acids

    @reffatty acidpercentageECL
    54133C14:02.714
    54133C16:030.616
    54133C18:08.518
    54133C16:1 ω7c5.315.819
    54133C18:1 ω9c31.317.769
    54133C18:2 ω6,9c/C18:0 ANTE21.717.724
  • type of FA analysis: whole cell analysis
  • method/protocol: CCUG

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
118335--++-+-+--++--------

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
118335----------+++----++-+/-+-----+--+/-+---++--++/------+-+/-

API rID32STR

@refADH Argbeta GLUbeta GARbeta GURalpha GALPALRIBMANSORLACTRERAFSACLARADARLCDEXVPAPPAbeta GALPyrAbeta NAGGTAHIPGLYGPULMALMELMLZMbeta DGTAGbeta MANURE
6062+------------------+--+---------

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinentsampling dategeographic locationisolation date
6062equine vaginal discharge, thoroughbred cross horseUnited KingdomGBREurope
54133Equine vaginal discharge,thoroughbred cross horseUnited KingdomGBREurope1995Inverness
118335Animal, Equine vaginal dischargeUnited KingdomGBREuropeInverness1997

isolation source categories

Cat1Cat2Cat3
#Host#Mammals#Equidae (Horse)
#Host Body-Site#Urogenital tract#Vagina
#Host Body Product#Fluids

taxonmaps

  • @ref: 69479
  • File name: preview.99_4321.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_178;96_2168;97_2624;98_3253;99_4321&stattab=map
  • Last taxonomy: Eremococcus coleocola subclade
  • 16S sequence: Y17780
  • Sequence Identity:
  • Total samples: 7963
  • soil counts: 327
  • aquatic counts: 282
  • animal counts: 7251
  • plant counts: 103

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
60621Risk group (German classification)
1183351Risk group (French classification)

Sequence information

16S sequences

  • @ref: 6062
  • description: Facklamia sp. 16S rRNA gene, strain M1832/95/2
  • accession: Y17780
  • length: 1385
  • database: ena
  • NCBI tax ID: 88132

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Eremococcus coleocola DSM 15696GCA_000428865scaffoldncbi1121871
66792Eremococcus coleocola DSM 156961121871.3wgspatric1121871
66792Eremococcus coleocola DSM 156962524614563draftimg1121871

GC content

@refGC-contentmethod
606240
2325240.00thermal denaturation, midpoint method (Tm)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
motileno96.036no
flagellatedno98.239no
gram-positiveyes92.905yes
anaerobicno73.508no
aerobicno95.191no
halophileyes87.961yes
spore-formingno94.893no
glucose-utilyes86.67no
thermophileno98.449no
glucose-fermentyes77.508no

External links

@ref: 6062

culture collection no.: CIP 106310, ATCC BAA 57, CCM 4939, DSM 15696, CCUG 38207

straininfo link

  • @ref: 69913
  • straininfo: 46271

literature

  • topic: Phylogeny
  • Pubmed-ID: 10555316
  • title: Characterization of a novel gram-positive, catalase-negative coccus from horses: description of Eremococcus coleocola gen. nov., sp. nov.
  • authors: Collins MD, Rodriguez Jovita M, Lawson PA, Falsen E, Foster G
  • journal: Int J Syst Bacteriol
  • DOI: 10.1099/00207713-49-4-1381
  • year: 1999
  • mesh: Animals, Bacterial Typing Techniques, Catalase/metabolism, Female, Genes, rRNA, Gram-Positive Bacterial Infections/microbiology/*veterinary, Gram-Positive Cocci/*classification/*genetics/isolation & purification, Horse Diseases/*microbiology, Horses, Molecular Sequence Data, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Vaginal Discharge/microbiology/*veterinary
  • topic2: Genetics

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
6062Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 15696)https://www.dsmz.de/collection/catalogue/details/culture/DSM-15696
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
23252Matthew D. Collins, Mar Rodriguez Jovita, Paul A. Lawson, Enevold Falsen, Geoffrey Foster10.1099/00207713-49-4-1381Characterization of a novel Gram-positive, catalase-negative coccus from horses: description of Eremococcus coleocola gen. nov. sp. nov.IJSEM 49: 1381-1385 199910555316
39489Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/18427
54133Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 38207)https://www.ccug.se/strain?id=38207
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66793Mukherjee et al.10.1038/nbt.3886GEBA: 1,003 reference genomes of bacterial and archaeal isolates expand coverage of the tree of life 35: 676-683 201728604660
68371Automatically annotated from API 50CH acid
68381Automatically annotated from API rID32STR
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
69913Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID46271.1StrainInfo: A central database for resolving microbial strain identifiers
118335Curators of the CIPCollection of Institut Pasteur (CIP 106310)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20106310