Strain identifier
BacDive ID: 22925
Type strain: 
Species: Mesobacillus persicus
Strain Designation: B48
Strain history: <- M. A. Amoozegar, Univ. Tehran, Iran; B48 <- M. Didari
NCBI tax ID(s): 930146 (species)
version 8.1 (current version)
General
@ref: 19303
BacDive-ID: 22925
DSM-Number: 25386
keywords: genome sequence, 16S sequence, Bacteria, aerobe, spore-forming, mesophilic, Gram-positive, rod-shaped
description: Mesobacillus persicus B48 is an aerobe, spore-forming, mesophilic bacterium that was isolated from soil around a hypersaline lake.
NCBI tax id
- NCBI tax id: 930146
- Matching level: species
strain history
- @ref: 19303
- history: <- M. A. Amoozegar, Univ. Tehran, Iran; B48 <- M. Didari
doi: 10.13145/bacdive22925.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/bacillota
- domain: Bacteria
- phylum: Bacillota
- class: Bacilli
- order: Caryophanales
- family: Bacillaceae
- genus: Mesobacillus
- species: Mesobacillus persicus
- full scientific name: Mesobacillus persicus (Didari et al. 2013) Patel and Gupta 2020
synonyms
- @ref: 20215
- synonym: Bacillus persicus
@ref: 19303
domain: Bacteria
phylum: Firmicutes
class: Bacilli
order: Bacillales
family: Bacillaceae
genus: Mesobacillus
species: Mesobacillus persicus
full scientific name: Mesobacillus persicus (Didari et al. 2013) Patel and Gupta 2020
strain designation: B48
type strain: yes
Morphology
cell morphology
| @ref | gram stain | cell length | cell width | cell shape | motility | confidence |
|---|---|---|---|---|---|---|
| 30708 | positive | 6 µm | 0.55 µm | rod-shaped | no | |
| 69480 | positive | 100 |
pigmentation
- @ref: 30708
- production: yes
Culture and growth conditions
culture medium
- @ref: 19303
- name: MEDIUM 514 PLUS ADDITIONAL SALT (DSMZ Medium 514b)
- growth: yes
- link: https://mediadive.dsmz.de/medium/514b
- composition: Name: MEDIUM 514 plus additional salt (DSMZ Medium 514b) Composition: NaCl 19.45 g/l Agar 17.5 g/l MgCl2 5.9 g/l Bacto peptone 5.0 g/l Na2SO4 3.24 g/l CaCl2 1.8 g/l Yeast extract 1.0 g/l KCl 0.55 g/l NaHCO3 0.16 g/l Fe(III) citrate 0.1 g/l KBr 0.08 g/l SrCl2 0.034 g/l H3BO3 0.022 g/l Na2HPO4 0.008 g/l Na-silicate 0.004 g/l NaF 0.0024 g/l (NH4)NO3 0.0016 g/l Distilled water
culture temp
| @ref | growth | type | temperature | range |
|---|---|---|---|---|
| 19303 | positive | growth | 28 | mesophilic |
| 30708 | positive | growth | 10-45 | |
| 30708 | positive | optimum | 35 | mesophilic |
culture pH
| @ref | ability | type | pH | PH range |
|---|---|---|---|---|
| 30708 | positive | growth | 06-09 | alkaliphile |
| 30708 | positive | optimum | 7.75 |
Physiology and metabolism
oxygen tolerance
- @ref: 30708
- oxygen tolerance: aerobe
spore formation
| @ref | spore formation | confidence |
|---|---|---|
| 30708 | yes | |
| 69481 | yes | 100 |
| 69480 | yes | 100 |
halophily
- @ref: 30708
- salt: NaCl
- growth: positive
- tested relation: optimum
- concentration: 2.5 %
metabolite utilization
| @ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
|---|---|---|---|---|
| 30708 | 16449 | alanine | + | carbon source |
| 30708 | 28757 | fructose | + | carbon source |
| 30708 | 17234 | glucose | + | carbon source |
| 30708 | 17754 | glycerol | + | carbon source |
| 30708 | 17306 | maltose | + | carbon source |
| 30708 | 29864 | mannitol | + | carbon source |
| 30708 | 33942 | ribose | + | carbon source |
| 30708 | 17992 | sucrose | + | carbon source |
| 30708 | 27082 | trehalose | + | carbon source |
| 30708 | 18222 | xylose | + | carbon source |
metabolite production
- @ref: 30708
- Chebi-ID: 16136
- metabolite: hydrogen sulfide
- production: yes
enzymes
| @ref | value | activity | ec |
|---|---|---|---|
| 30708 | catalase | + | 1.11.1.6 |
| 30708 | cytochrome oxidase | + | 1.9.3.1 |
Isolation, sampling and environmental information
isolation
- @ref: 19303
- sample type: soil around a hypersaline lake
- geographic location: Lake Aran-Bidgol
- country: Iran
- origin.country: IRN
- continent: Asia
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Environmental | #Aquatic | #Lake (large) |
| #Environmental | #Terrestrial | #Soil |
| #Condition | #Saline |
taxonmaps
- @ref: 69479
- File name: preview.99_20892.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_3;96_292;97_834;98_15782;99_20892&stattab=map
- Last taxonomy: Bacillaceae
- 16S sequence: HQ433471
- Sequence Identity:
- Total samples: 53
- soil counts: 20
- aquatic counts: 25
- animal counts: 8
Safety information
risk assessment
- @ref: 19303
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 19303
- description: Bacillus persicus strain B48 16S ribosomal RNA gene, partial sequence
- accession: HQ433471
- length: 1458
- database: ena
- NCBI tax ID: 930146
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Mesobacillus persicus B48,IBRC-M 10115,DSM 25386,CECT 8001 | GCA_900109925 | scaffold | ncbi | 930146 |
| 66792 | Bacillus persicus strain B48,IBRC-M 10115,DSM 25386,CECT 8001 | 930146.3 | wgs | patric | 930146 |
| 66792 | Bacillus persicus DSM 25386 | 2651870116 | draft | img | 930146 |
GC content
| @ref | GC-content | method |
|---|---|---|
| 19303 | 40.1 | high performance liquid chromatography (HPLC) |
| 30708 | 40.1 |
Genome-based predictions
predictions
| trait | prediction | confidence | training_data |
|---|---|---|---|
| spore-forming | yes | 100 | no |
| glucose-ferment | no | 91.718 | no |
| motile | no | 85.667 | yes |
| flagellated | no | 82.683 | yes |
| gram-positive | yes | 90.765 | yes |
| anaerobic | no | 96.381 | yes |
| aerobic | yes | 85.176 | yes |
| halophile | yes | 75.624 | no |
| spore-forming | yes | 90.457 | no |
| thermophile | no | 98.151 | yes |
| glucose-util | yes | 84.76 | no |
External links
@ref: 19303
culture collection no.: DSM 25386, CECT 8001, IBRC-M 10115
straininfo link
- @ref: 87173
- straininfo: 377847
literature
| topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
|---|---|---|---|---|---|---|---|---|
| Phylogeny | 22771682 | Bacillus persicus sp. nov., a halophilic bacterium from a hypersaline lake. | Didari M, Amoozegar MA, Bagheri M, Mehrshad M, Schumann P, Sproer C, Sanchez-Porro C, Ventosa A | Int J Syst Evol Microbiol | 10.1099/ijs.0.042689-0 | 2012 | Bacillus/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Diaminopimelic Acid/analysis, Fatty Acids/analysis, Iran, Lakes/*microbiology, Molecular Sequence Data, Nucleic Acid Hybridization, Phospholipids/analysis, *Phylogeny, RNA, Ribosomal, 16S/genetics, Salinity, Sequence Analysis, DNA, *Water Microbiology | Genetics |
| Phylogeny | 29458563 | Bacillus fermenti sp. nov., an indigo-reducing obligate alkaliphile isolated from indigo fermentation liquor for dyeing. | Hirota K, Nishita M, Tu Z, Matsuyama H, Yumoto I | Int J Syst Evol Microbiol | 10.1099/ijsem.0.002636 | 2018 | Bacillus/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, *Coloring Agents, DNA, Bacterial/genetics, Fatty Acids/chemistry, *Fermentation, Indigo Carmine/analysis, Japan, Nucleic Acid Hybridization, *Phylogeny, Polygonum/*microbiology, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Vitamin K 2/analogs & derivatives/chemistry | Transcriptome |
Reference
| @id | authors | catalogue | doi/url | title | ID_cross_reference | pubmed | journal |
|---|---|---|---|---|---|---|---|
| 19303 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 25386) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-25386 | ||||
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | ||||
| 30708 | Barberan A, Caceres Velazquez H, Jones S, Fierer N. | 10.1128/mSphere.00237-17 | Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information | 27039 | 28776041 | ||
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | ||||
| 66794 | Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg | 10.1093/nar/gkaa1025 | BRENDA, the ELIXIR core data resource in 2021: new developments and updates | 33211880 | Nucleic Acids Res. 49: D498-D508 2020 | ||
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | ||||
| 69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | ||||
| 69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | 10.21203/rs.3.rs-2527258/v1 | Predictions based on the model GenomeNet Sporulation v. 1 | ||||
| 87173 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID377847.1 | StrainInfo: A central database for resolving microbial strain identifiers |