Strain identifier

BacDive ID: 2269

Type strain: Yes

Species: Carnobacterium viridans

Strain Designation: MPL-11

Strain history: CIP <- 2002, R.A. Holley, Manitoba Univ., Winnipeg, Manitoba, Canada: strain MPL-11 <- M. Peirson

NCBI tax ID(s): 174587 (species)

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General

@ref: 5360

BacDive-ID: 2269

DSM-Number: 14451

keywords: genome sequence, 16S sequence, Bacteria, facultative anaerobe, Gram-positive

description: Carnobacterium viridans MPL-11 is a facultative anaerobe, Gram-positive bacterium that was isolated from vacuum packed ham .

NCBI tax id

  • NCBI tax id: 174587
  • Matching level: species

strain history

@refhistory
5360<- R.A. Holley; MPL-11 <- M. Peirson
67770DSM 14451 <-- R. A. Holley MPL-11 <-- M. Peirson.
123859CIP <- 2002, R.A. Holley, Manitoba Univ., Winnipeg, Manitoba, Canada: strain MPL-11 <- M. Peirson

doi: 10.13145/bacdive2269.20240916.9.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Carnobacteriaceae
  • genus: Carnobacterium
  • species: Carnobacterium viridans
  • full scientific name: Carnobacterium viridans Holley et al. 2002

@ref: 5360

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Carnobacteriaceae

genus: Carnobacterium

species: Carnobacterium viridans

full scientific name: Carnobacterium viridans Holley et al. 2002

strain designation: MPL-11

type strain: yes

Morphology

cell morphology

  • @ref: 123859
  • gram stain: positive
  • cell shape: rod-shaped
  • motility: no

colony morphology

  • @ref: 123859
  • hemolysis ability: 1

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
5360TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)yeshttps://mediadive.dsmz.de/medium/92Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water
38227MEDIUM 29- Brain heart agaryesDistilled water make up to (1000.000 ml);Brain heart infusion agar (52.000 g)
123859CIP Medium 29yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=29
123859CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperature
5360positivegrowth30
38227positivegrowth25
67770positivegrowth30
123859positivegrowth5-30
123859nogrowth37
123859nogrowth41

Physiology and metabolism

oxygen tolerance

  • @ref: 123859
  • oxygen tolerance: facultative anaerobe

halophily

@refsaltgrowthtested relationconcentration
123859NaClpositivegrowth0-6 %
123859NaClnogrowth8 %
123859NaClnogrowth10 %

murein

  • @ref: 5360
  • murein short key: A31
  • type: A1gamma m-Dpm-direct

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate-builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-lyxose-builds acid from62318
68371turanose-builds acid from32528
68371gentiobiose-builds acid from28066
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371starch-builds acid from28017
68371raffinose-builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371melibiose-builds acid from28053
68371salicin-builds acid from17814
68371esculin-builds acid from4853
68371arbutin-builds acid from18305
68371amygdalin-builds acid from27613
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol-builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371D-ribose-builds acid from16988
68371L-arabinose-builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754
123859citrate-carbon source16947
123859esculin+hydrolysis4853
123859hippurate+hydrolysis606565
123859nitrate-builds gas from17632
123859nitrate-reduction17632
123859nitrite-builds gas from16301
123859nitrite-reduction16301

metabolite production

  • @ref: 123859
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin+3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase-3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)-
68382esterase (C 4)+
68382alkaline phosphatase-3.1.3.1
123859oxidase-
123859beta-galactosidase+3.2.1.23
123859alcohol dehydrogenase-1.1.1.1
123859gelatinase-
123859amylase-
123859DNase-
123859caseinase-3.4.21.50
123859catalase-1.11.1.6
123859tween esterase-
123859gamma-glutamyltransferase-2.3.2.2
123859lecithinase-
123859lipase-
123859lysine decarboxylase-4.1.1.18
123859ornithine decarboxylase-4.1.1.17
123859phenylalanine ammonia-lyase-4.3.1.24
123859protease-
123859tryptophan deaminase-
123859urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
123859--+------+++--------

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
123859---------+/-+/-+/-+/---------+/-----+/-+/-+/--+/-+/----------+/--------

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
123859+++++--+-++++--+--+-----------+----------------------------+---------------------------------------

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinentisolation date
5360vacuum packed ham (bologna)CanadaCANNorth America
67770Refrigerated vacuum-packaged bologna
123859Vacuum packed hamCanadaCANNorth America2001

isolation source categories

Cat1Cat2Cat3
#Engineered#Food production#Bottled, canned, packed food
#Engineered#Food production#Meat

taxonmaps

  • @ref: 69479
  • File name: preview.99_51.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_13;96_41;97_43;98_47;99_51&stattab=map
  • Last taxonomy: Carnobacterium
  • 16S sequence: LC145568
  • Sequence Identity:
  • Total samples: 1950
  • soil counts: 293
  • aquatic counts: 344
  • animal counts: 1241
  • plant counts: 72

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
53601Risk group (German classification)
1238591Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
5360Carnobacterium viridans 16S ribosomal RNA gene, partial sequenceAF4256081465nuccore174587
67770Carnobacterium viridans gene for 16S ribosomal RNA, partial sequence, strain: JCM 12222LC1455681497nuccore174587

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Carnobacterium viridans MPL-11GCA_900102725contigncbi174587
66792Carnobacterium viridans strain MPL-11174587.3wgspatric174587
66792Carnobacterium viridans MPL-11, DSM 144512634166280draftimg174587

Genome-based predictions

predictions

@reftraitmodeldescriptionpredictionconfidencetraining_data
69480gram-positivegram-positivePositive reaction to Gram-stainingyes89.362no
69480anaerobicanaerobicAbility to grow under anoxygenic conditions (including facultative anaerobes)no88.887no
69480spore-formingspore-formingAbility to form endo- or exosporesno70.981no
69480aerobicaerobicAbility to grow under oxygenic conditions (including facultative aerobes)no85.137yes
69480thermophilicthermophileAbility to grow at temperatures above or equal to 45°Cno96.016yes
69480flagellatedmotile2+Ability to perform flagellated movementno85.271no

External links

@ref: 5360

culture collection no.: DSM 14451, ATCC BAA-336, JCM 12222, CIP 107728

straininfo link

  • @ref: 71827
  • straininfo: 100584

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny12361300Carnobacterium viridans sp. nov., an alkaliphilic, facultative anaerobe isolated from refrigerated, vacuum-packed bologna sausage.Holley RA, Guan TY, Peirson M, Yost CKInt J Syst Evol Microbiol10.1099/00207713-52-5-18812002DNA, Bacterial/genetics, DNA, Ribosomal/genetics, *Food Microbiology, Gram-Positive Asporogenous Rods/*classification/genetics/*isolation & purification/metabolism, Meat Products/*microbiology, Molecular Sequence Data, Phenotype, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, RefrigerationBiotechnology
Pathogenicity12915033Thermal resistances and lactate and diacetate sensitivities of bacteria causing bologna discolouration.Peirson MD, Guan TY, Holley RAInt J Food Microbiol10.1016/s0168-1605(02)00548-22003Acetates/*pharmacology, Animals, Colony Count, Microbial, Color, Colorimetry, Disinfectants/*pharmacology, Food Handling/methods, Food Microbiology, Hot Temperature, Lactobacillaceae/drug effects/*growth & development, Lactobacillus/drug effects/growth & development, Meat Products/*microbiology/standards, Sodium Lactate/*pharmacology, Streptococcaceae/drug effects/*growth & development, SwineBiotechnology

Reference

@idauthorscataloguedoi/urltitle
5360Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 14451)https://www.dsmz.de/collection/catalogue/details/culture/DSM-14451
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
38227Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/5196
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
71827Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID100584.1StrainInfo: A central database for resolving microbial strain identifiers
123859Curators of the CIPCollection of Institut Pasteur (CIP 107728)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20107728