Strain identifier

BacDive ID: 22669

Type strain: No

Species: Herpetosiphon sp.

Strain Designation: Hp g323

NCBI tax ID(s): 71864 (species)

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General

@ref: 18281

BacDive-ID: 22669

keywords: Bacteria

description: Herpetosiphon sp. Hp g323 is a bacterium that was isolated from soil with plant residues.

NCBI tax id

  • NCBI tax id: 71864
  • Matching level: species

doi: 10.13145/bacdive22669.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/chloroflexota
  • domain: Bacteria
  • phylum: Chloroflexota
  • class: Chloroflexia
  • order: Herpetosiphonales
  • family: Herpetosiphonaceae
  • genus: Herpetosiphon
  • species: Herpetosiphon sp.
  • full scientific name: Herpetosiphon Holt and Lewin 1968 (Approved Lists 1980)

@ref: 18281

domain: Bacteria

phylum: Chloroflexi

class: Chloroflexi, not validated

order: Herpetosiphonales

family: Herpetosiphonaceae, not validated

genus: Herpetosiphon

species: Herpetosiphon sp.

full scientific name: Herpetosiphon sp.

strain designation: Hp g323

type strain: no

Culture and growth conditions

culture medium

@refnamegrowthcomposition
18281COLE AGARyesIngredients: CaCl2 x 2 H2O 1.3g Agar (Difco) 15.0g Distilled water 1000.0ml Preparation / Remarks: pH 7.2 (KOH) autoclaved Pour the plates and streak living E.coli cells
18281Myx MEDIUMnoIngredients: Peptone (Difco) 1% Glucose 1% Yeast extract (Difco) 0.1% Phosphate buffer 0.01 M pH 6.5 Aerobacter aerogenes (sterilized with formalin) 500 mg/L (dry weight) as described by Kerr, N.S. (1963) The Growth of Myxamoebae of the True Slime Mould, Didymium nigripes, in Axenic Culture. J Gen Microbiol 32: 409-416.
18281TOLI AGAR Kan14yesIngredients: Baker's yeast 0.5% (based on fresh weight) CaCl2 0.1% Agar 1.5% Vitamine B12 0.5 µg/ml Preparation / Remarks: pH 7.2 autoclaved Combine with 1 mg/L Kanamycin-sulfate (sterilized by filtration) Pour the plates and streak autoclaved E.coli cells
18281TOLI AGAR Kan4yesIngredients: Agar (Difco) 1.5% CaCl2 0.1% Cycloheximide 2.5 mg per 100 ml Preparation / Remarks: pH 7.2 (KOH) autoclaved w-agar with 2.5 mg of cycloheximide per 100 ml, the antibiotic is filter sterilized and added to the agar just before pouring Cycloheximide does not seem to affect growth of myxobakteria, whereas fungi and amoebae are often inhibited. Combine with 250 mg/L Kanamycin-sulfate (sterilized by filtration) Pour the plates and streak autoclaved E.coli cells
18281VY/2 AGARyesIngredients: Baker's yeast 0.5% (based on fresh weight) CaCl2 0.1% Agar 1.5% Vitamin B12 0.05 % Preparation / Remarks: pH 7.2 autoclaved

Physiology and metabolism

observation

@refobservation
18281Ability for Yeast lysis
18281Ability for E. coli lysis

antibiotic resistance

@refChEBImetaboliteis antibioticis resistantresistance conc.group ID
182816104kanamycinyesyes1 µg/mL
182816104kanamycinyesyes250 µg/mL3
1828127641cycloheximideyesyes25 µg/mL3

Isolation, sampling and environmental information

isolation

  • @ref: 18281
  • sample type: soil with plant residues
  • sampling date: 1995-03-01
  • geographic location: Lucknow, Uttar Pradesh
  • country: India
  • origin.country: IND
  • continent: Asia

isolation source categories

Cat1Cat2Cat3
#Environmental#Terrestrial#Soil
#Host#Plants

External links

@ref: 18281

Reference

@idauthorstitledoi/url
18281Reichenbach, H.Collection description and documentation of myxobacteria by H. Reichenbach, HZI (formerly GBF); Collection curr. located at the DSMZ
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332