Strain identifier
BacDive ID: 2229
Type strain: 
Species: Sulfurospirillum multivorans
Strain history: DSM 12446 <-- A. Neumann and G. Diekert.
NCBI tax ID(s): 1150621 (strain), 66821 (species)
version 8.1 (current version)
General
@ref: 4722
BacDive-ID: 2229
DSM-Number: 12446
keywords: genome sequence, 16S sequence, Bacteria, anaerobe, mesophilic, motile
description: Sulfurospirillum multivorans DSM 12446 is an anaerobe, mesophilic, motile bacterium that was isolated from activated sludge.
NCBI tax id
| NCBI tax id | Matching level |
|---|---|
| 1150621 | strain |
| 66821 | species |
strain history
| @ref | history |
|---|---|
| 4722 | <- G. Diekert, Universität Stuttgart, Institut für Mikrobiologie, Stuttgart, Germany <- G. Diekert {1993} |
| 67770 | DSM 12446 <-- A. Neumann and G. Diekert. |
doi: 10.13145/bacdive2229.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Epsilonproteobacteria
- order: Campylobacterales
- family: Campylobacteraceae
- genus: Sulfurospirillum
- species: Sulfurospirillum multivorans
- full scientific name: Sulfurospirillum multivorans (Scholz-Muramatsu et al. 2002) Luijten et al. 2003
synonyms
- @ref: 20215
- synonym: Dehalospirillum multivorans
@ref: 4722
domain: Bacteria
phylum: Proteobacteria
class: Epsilonproteobacteria
order: Campylobacterales
family: Campylobacteraceae
genus: Sulfurospirillum
species: Sulfurospirillum multivorans
full scientific name: Sulfurospirillum multivorans (Scholz-Muramatsu et al. 2002) Luijten et al. 2003
type strain: yes
Morphology
cell morphology
| @ref | motility | confidence | gram stain |
|---|---|---|---|
| 69480 | yes | 91.305 | |
| 69480 | 100 | negative |
Culture and growth conditions
culture medium
- @ref: 4722
- name: DEHALOSPIRILLUM MEDIUM (DSMZ Medium 833)
- growth: yes
- link: https://mediadive.dsmz.de/medium/833
- composition: Name: DEHALOSPIRILLUM MEDIUM (DSMZ Medium 833) Composition: Na2-fumarate 6.38086 g/l Na-pyruvate 4.48654 g/l Yeast extract 1.99402 g/l Na2CO3 1.74477 g/l Na2SO4 0.697906 g/l KCl 0.498504 g/l MgCl2 x 6 H2O 0.398803 g/l NaCl 0.249252 g/l NH4Cl 0.249252 g/l KH2PO4 0.199402 g/l CaCl2 x 2 H2O 0.149551 g/l L-Cysteine HCl x H2O 0.0498504 g/l FeSO4 x 7 H2O 0.0128187 g/l HCl 0.00249252 g/l FeCl2 x 4 H2O 0.00149551 g/l NaOH 0.000498504 g/l Sodium resazurin 0.000498504 g/l Pyridoxine hydrochloride 0.000299103 g/l Nicotinic acid 0.000199402 g/l Thiamine-HCl x 2 H2O 0.000199402 g/l CoCl2 x 6 H2O 0.000189432 g/l Vitamin B12 9.97009e-05 g/l Calcium pantothenate 9.97009e-05 g/l MnCl2 x 4 H2O 9.97009e-05 g/l p-Aminobenzoic acid 7.97607e-05 g/l ZnCl2 6.97906e-05 g/l (DL)-alpha-Lipoic acid 4.98504e-05 g/l Calcium D-(+)-pantothenate 4.98504e-05 g/l Riboflavin 4.98504e-05 g/l Thiamine HCl 4.98504e-05 g/l Na2MoO4 x 2 H2O 3.58923e-05 g/l NiCl2 x 6 H2O 2.39282e-05 g/l D-(+)-biotin 1.99402e-05 g/l Folic acid 1.99402e-05 g/l Biotin 1.99402e-05 g/l H3BO3 5.98205e-06 g/l Na2WO4 x 2 H2O 3.98804e-06 g/l Na2SeO3 x 5 H2O 2.99103e-06 g/l CuCl2 x 2 H2O 1.99402e-06 g/l Potassium phosphate buffer H2SO4 Distilled water
culture temp
| @ref | growth | type | temperature | range |
|---|---|---|---|---|
| 4722 | positive | growth | 25 | mesophilic |
| 67770 | positive | growth | 25 | mesophilic |
Physiology and metabolism
oxygen tolerance
| @ref | oxygen tolerance | confidence |
|---|---|---|
| 4722 | anaerobe | |
| 69480 | anaerobe | 98.648 |
spore formation
| @ref | spore formation | confidence |
|---|---|---|
| 69481 | no | 100 |
| 69480 | no | 99.995 |
observation
- @ref: 67770
- observation: quinones: Menaquinone
Isolation, sampling and environmental information
isolation
| @ref | sample type | country | origin.country | continent |
|---|---|---|---|---|
| 4722 | activated sludge | Germany | DEU | Europe |
| 67770 | Activated sludge |
isolation source categories
- Cat1: #Engineered
- Cat2: #Waste
- Cat3: #Activated sludge
taxonmaps
- @ref: 69479
- File name: preview.99_2491.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_283;96_1116;97_1309;98_1939;99_2491&stattab=map
- Last taxonomy: Sulfurospirillum
- 16S sequence: X82931
- Sequence Identity:
- Total samples: 740
- soil counts: 98
- aquatic counts: 591
- animal counts: 38
- plant counts: 13
Safety information
risk assessment
- @ref: 4722
- biosafety level: 1
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
| @ref | description | accession | length | database | NCBI tax ID |
|---|---|---|---|---|---|
| 20218 | Sulfurospirillum multivorans strain DSM 12446 16S ribosomal RNA gene, partial sequence; 16S-23S ribosomal RNA intergenic spacer, complete sequence; and 23S ribosomal RNA gene, partial sequence | DQ834670 | 742 | ena | 1150621 |
| 4722 | Dehalospirillum multivorans 16S rRNA gene | X82931 | 1464 | ena | 1150621 |
Genome sequences
| @ref | description | accession | assembly level | database | NCBI tax ID |
|---|---|---|---|---|---|
| 66792 | Sulfurospirillum multivorans DSM 12446 | GCA_000568815 | complete | ncbi | 1150621 |
| 66792 | Sulfurospirillum multivorans DSM 12446 | 1150621.9 | complete | patric | 1150621 |
| 66792 | Sulfurospirillum multivorans DSM 12446 | 2558860208 | complete | img | 1150621 |
GC content
- @ref: 4722
- GC-content: 41.5
- method: high performance liquid chromatography (HPLC)
Genome-based predictions
predictions
| trait | prediction | confidence | training_data |
|---|---|---|---|
| spore-forming | no | 100 | no |
| motile | yes | 87.447 | no |
| flagellated | yes | 74.981 | no |
| gram-positive | no | 97.819 | no |
| anaerobic | yes | 96.471 | yes |
| halophile | no | 94.313 | no |
| spore-forming | no | 91.559 | no |
| thermophile | no | 99.566 | no |
| glucose-util | no | 76.683 | no |
| aerobic | no | 96.798 | no |
| glucose-ferment | no | 90.043 | no |
External links
@ref: 4722
culture collection no.: DSM 12446, JCM 15788, NBRC 109480
straininfo link
- @ref: 71792
- straininfo: 49289
literature
| topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
|---|---|---|---|---|---|---|---|---|
| Phylogeny | 12807201 | Description of Sulfurospirillum halorespirans sp. nov., an anaerobic, tetrachloroethene-respiring bacterium, and transfer of Dehalospirillum multivorans to the genus Sulfurospirillum as Sulfurospirillum multivorans comb. nov. | Luijten MLGC, de Weert J, Smidt H, Boschker HTS, de Vos WM, Schraa G, Stams AJM | Int J Syst Evol Microbiol | 10.1099/ijs.0.02417-0 | 2003 | Anaerobiosis, Bacterial Typing Techniques, DNA, Ribosomal/analysis, Environmental Pollutants/*metabolism, Epsilonproteobacteria/*classification/*genetics/growth & development/metabolism, Fatty Acids/analysis, Molecular Sequence Data, Nucleic Acid Hybridization, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Tetrachloroethylene/*metabolism | Metabolism |
| Genetics | 33004458 | Complete Genome Sequence of Sulfurospirillum Strain ACSTCE, a Tetrachloroethene-Respiring Anaerobe Isolated from Contaminated Soil. | Huo L, Yang Y, Lv Y, Li X, Loffler FE, Yan J | Microbiol Resour Announc | 10.1128/MRA.00941-20 | 2020 | Phylogeny |
Reference
| @id | authors | catalogue | doi/url | title | journal | pubmed |
|---|---|---|---|---|---|---|
| 4722 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 12446) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-12446 | |||
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
| 20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | |
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
| 67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||||
| 69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
| 69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
| 69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | 10.21203/rs.3.rs-2527258/v1 | Predictions based on the model GenomeNet Sporulation v. 1 | |||
| 71792 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID49289.1 | StrainInfo: A central database for resolving microbial strain identifiers |