Strain identifier

BacDive ID: 22064

Type strain: No

Species: Herpetosiphon sp.

Strain Designation: Hp g182

NCBI tax ID(s): 71864 (species)

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General

@ref: 18281

BacDive-ID: 22064

keywords: Bacteria

description: Herpetosiphon sp. Hp g182 is a bacterium that was isolated from soil with plant residues.

NCBI tax id

  • NCBI tax id: 71864
  • Matching level: species

doi: 10.13145/bacdive22064.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/chloroflexota
  • domain: Bacteria
  • phylum: Chloroflexota
  • class: Chloroflexia
  • order: Herpetosiphonales
  • family: Herpetosiphonaceae
  • genus: Herpetosiphon
  • species: Herpetosiphon sp.
  • full scientific name: Herpetosiphon Holt and Lewin 1968 (Approved Lists 1980)

@ref: 18281

domain: Bacteria

phylum: Chloroflexi

class: Chloroflexi, not validated

order: Herpetosiphonales

family: Herpetosiphonaceae, not validated

genus: Herpetosiphon

species: Herpetosiphon sp.

full scientific name: Herpetosiphon sp.

strain designation: Hp g182

type strain: no

Culture and growth conditions

culture medium

@refnamegrowthcomposition
18281CA2 AGARyesIngredients: Stock solution 1 (in 100ml): KNO3 7.5g K2HPO4 7.5g Stock solution 2 (in 100ml): MgSO4 x 7H2O 1.5g Stock solution 3 (in 100ml): CaCl2 0.2g FeCl3 0.15g Basal medium: Agar 1.5% Preparation / Remarks: after autoclaving to: a) 1% (v/v) of the 3 stock solutions b) 1% glucose c) trace elements
18281CHITIN 6 AGARyesIngredients: 1. Basal agar: Casitone (Difco) 0.1% Yeast extract (Difco) 0.05% MgSO4 x 7H2O 0.1% Agar 1.2% Preparation / Remarks: pH 7.2 After autoclaving add 30 mL of sterile Chitin suspension (22.5 g/L Chitin in H2O) to 100 mL of the medium 2. Covering agar (thin layer): MgSO4 x 7H2O 0.1% K2HPO4 0.02% Agar 1.5% Preparation / Remarks: pH 7.2 After autoclaving add 30 mL of sterile Chitin suspension (22.5 g/L Chitin in H2O) to 100 mL of the medium
18281COLE AGARyesIngredients: CaCl2 x 2 H2O 1.3g Agar (Difco) 15.0g Distilled water 1000.0ml Preparation / Remarks: pH 7.2 (KOH) autoclaved Pour the plates and streak living E.coli cells
18281SKIM MILK 2 AGARyesIngredients: cy-Agar 300 ml Skim milk (Oxoid) 15g H2O 60 ml Preparation / Remarks: 300 ml cy-agar autoclaved. Separately 15 g skim milk (Oxoid) in 60 ml water and exactly 15 min autoclaving at 115 °C. Mix the two and pour a thin layer on CA2-plates.
18281SKIM MILK 3 AGARyesIngredients: cy-Agar 300 ml Skim milk (Oxoid) 15g H2O 60 ml Preparation / Remarks: 300 ml cy-agar autoclaved. Separately 15 g skim milk (Oxoid) in 60 ml water and exactly 15 min autoclaving at 115 °C. Mix the two and pour a thin layer on CYG2-plates.
18281VY/2 AGARyesIngredients: Baker's yeast 0.5% (based on fresh weight) CaCl2 0.1% Agar 1.5% Vitamin B12 0.05 % Preparation / Remarks: pH 7.2 autoclaved
18281XYLAN 3 AGARyesIngredients: Xylan 0.5% (NH4)2SO4 0.05% Agar 1.5% Preparation / Remarks: pH 7.2 Pour after autoclaving as a thin cover layer on CyG2 Grundagar Xylan (Serva pure molecular weight ~ 25000)

Physiology and metabolism

observation

@refobservation
18281Ability for casein degradation
18281Ability for chitin degradation
18281Ability for Yeast lysis
18281Ability for E. coli lysis

Isolation, sampling and environmental information

isolation

  • @ref: 18281
  • sample type: soil with plant residues
  • sampling date: 1988-03-01
  • geographic location: near Uschi
  • country: South Africa
  • origin.country: ZAF
  • continent: Africa

isolation source categories

Cat1Cat2Cat3
#Environmental#Terrestrial#Soil
#Host#Plants

External links

@ref: 18281

Reference

@idauthorstitledoi/url
18281Reichenbach, H.Collection description and documentation of myxobacteria by H. Reichenbach, HZI (formerly GBF); Collection curr. located at the DSMZ
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332