Archive BacDiveID:10.13145/bacdive22056.20230509.8.1

Strain identifier

BacDive ID: 22056

Type strain:

Species: Herpetosiphon sp.

Strain Designation: Hp g169

NCBI tax ID(s): 71864 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.

General

@ref: 18281

BacDive-ID: 22056

keywords: Bacteria

description: Herpetosiphon sp. Hp g169 is a bacterium that was isolated from sand with shells and plant residues.

NCBI tax id

NCBI tax id: 71864
Matching level: species

doi: 10.13145/bacdive22056.20230509.8.1

Name and taxonomic classification

LPSN

@ref: 20215
description: domain/bacteria
keyword: phylum/chloroflexota
domain: Bacteria
phylum: Chloroflexota
class: Chloroflexia
order: Herpetosiphonales
family: Herpetosiphonaceae
genus: Herpetosiphon
species: Herpetosiphon sp.
full scientific name: Herpetosiphon Holt and Lewin 1968 (Approved Lists 1980)

@ref: 18281

domain: Bacteria

phylum: Chloroflexi

class: Chloroflexi, not validated

order: Herpetosiphonales

family: Herpetosiphonaceae, not validated

genus: Herpetosiphon

species: Herpetosiphon sp.

full scientific name: Herpetosiphon sp.

strain designation: Hp g169

type strain: no

Culture and growth conditions

culture medium

@ref	name	growth	composition
18281	CHITIN 6 AGAR	yes	Ingredients: 1. Basal agar: Casitone (Difco) 0.1% Yeast extract (Difco) 0.05% MgSO4 x 7H2O 0.1% Agar 1.2% Preparation / Remarks: pH 7.2 After autoclaving add 30 mL of sterile Chitin suspension (22.5 g/L Chitin in H2O) to 100 mL of the medium 2. Covering agar (thin layer): MgSO4 x 7H2O 0.1% K2HPO4 0.02% Agar 1.5% Preparation / Remarks: pH 7.2 After autoclaving add 30 mL of sterile Chitin suspension (22.5 g/L Chitin in H2O) to 100 mL of the medium
18281	CHITIN 7 AGAR	yes	Covering agar (thin layer): MgSO4 x 7H2O 0.1% K2HPO4 0.02% Agar 1.5% Preparation / Remarks: pH 7.2 After autoclaving add 30 mL of sterile Chitin suspension (22.5 g/L Chitin in H2O) to 100 mL of the medium and pour on w-agar plates
18281	COLE AGAR	yes	Ingredients: CaCl2 x 2 H2O 1.3g Agar (Difco) 15.0g Distilled water 1000.0ml Preparation / Remarks: pH 7.2 (KOH) autoclaved Pour the plates and streak living E.coli cells
18281	HP17 AGAR	yes	Ingedients: Na-glutaminate 0.5% MgSO4x7H2O 0.1% Agar 1.5% autoclaved separately: Glucose 0.2% Preparation / Remarks: pH 7
18281	HP18 AGAR	yes	Ingredients: Na-glutaminate 0.1% MgSO4x7H2O 0.1% Agar 1.5% Glucose 0.1% Preparation / Remarks: sterile traceelement-solution 0.1 ml (100 ml after autoclaving to, pH 7.2) Autoclaving glucose seperatly
18281	PROBION L AGAR	yes	Ingredients: Probion L 600 (Hoechst) 0.8% MgSO4 x 7H2O 0.1% CaCl2 x 2H2O 0.1% Agar 1.5% Preparation / Remarks: pH 7.2 autoclaved
18281	SKIM MILK 1 AGAR	yes	Ingredients: cy-Agar 300 ml Skim milk (Oxoid) 15g H2O 60 ml Preparation / Remarks: 300 ml cy-agar autoclaved. Separately 15 g skim milk (Oxoid) in 60 ml water and exactly 15 min autoclaving at 115 °C. Mix the two and pour a thin layer on cy-plates.
18281	VY/2 AGAR	yes	Ingredients: Baker's yeast 0.5% (based on fresh weight) CaCl2 0.1% Agar 1.5% Vitamin B12 0.05 % Preparation / Remarks: pH 7.2 autoclaved

Physiology and metabolism

observation

@ref	observation
18281	Ability for chitin degradation
18281	Ability for protein degradation
18281	Ability for Yeast lysis
18281	Ability for E. coli lysis

Isolation, sampling and environmental information

isolation

@ref: 18281
sample type: sand with shells and plant residues
sampling date: 1987-06-01
geographic location: Kos Island
country: Greece
origin.country: GRC
continent: Europe

isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Terrestrial	#Sandy
#Host	#Plants

External links

@ref: 18281

Reference

@id	authors	title	doi/url
18281	Reichenbach, H.	Collection description and documentation of myxobacteria by H. Reichenbach, HZI (formerly GBF); Collection curr. located at the DSMZ
20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.	List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ	10.1099/ijsem.0.004332