Strain identifier

BacDive ID: 22021

Type strain: No

Species: Cystobacter sp.

Strain Designation: Cb o11

NCBI tax ID(s): 1965334 (species)

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General

@ref: 18281

BacDive-ID: 22021

keywords: Bacteria

description: Cystobacter sp. Cb o11 is a bacterium that builds a fruiting body and was isolated from plant residues with little soil.

NCBI tax id

  • NCBI tax id: 1965334
  • Matching level: species

doi: 10.13145/bacdive22021.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Deltaproteobacteria
  • order: Myxococcales
  • family: Archangiaceae
  • genus: Cystobacter
  • species: Cystobacter sp.
  • full scientific name: Cystobacter Schroeter 1886 (Approved Lists 1980)

@ref: 18281

domain: Bacteria

phylum: Proteobacteria

class: Deltaproteobacteria

order: Myxococcales

family: Cystobacteraceae

genus: Cystobacter

species: Cystobacter sp.

full scientific name: Cystobacter sp.

strain designation: Cb o11

type strain: no

Morphology

multicellular morphology

  • @ref: 18281
  • forms multicellular complex: yes
  • complex name: fruiting body

Culture and growth conditions

culture medium

@refnamegrowthcomposition
18281CHITIN 6 AGARyesIngredients: 1. Basal agar: Casitone (Difco) 0.1% Yeast extract (Difco) 0.05% MgSO4 x 7H2O 0.1% Agar 1.2% Preparation / Remarks: pH 7.2 After autoclaving add 30 mL of sterile Chitin suspension (22.5 g/L Chitin in H2O) to 100 mL of the medium 2. Covering agar (thin layer): MgSO4 x 7H2O 0.1% K2HPO4 0.02% Agar 1.5% Preparation / Remarks: pH 7.2 After autoclaving add 30 mL of sterile Chitin suspension (22.5 g/L Chitin in H2O) to 100 mL of the medium
18281CHITIN 7 AGARyesCovering agar (thin layer): MgSO4 x 7H2O 0.1% K2HPO4 0.02% Agar 1.5% Preparation / Remarks: pH 7.2 After autoclaving add 30 mL of sterile Chitin suspension (22.5 g/L Chitin in H2O) to 100 mL of the medium and pour on w-agar plates
18281COLE AGARyesIngredients: CaCl2 x 2 H2O 1.3g Agar (Difco) 15.0g Distilled water 1000.0ml Preparation / Remarks: pH 7.2 (KOH) autoclaved Pour the plates and streak living E.coli cells
18281CY-AGARyesIngredients: Casitone(Difco) 0.3% yeast extract 0.1% CaCl2 0.1% Agar 1.5% Preparation / Remarks: pH 7.2 (KOH) autoclaved
18281TOLI AGARyesIngredients: CaCl2 x 2 H2O1.3g Agar (Difco)15.0g Distilled water1000.0ml Preparation / Remarks: pH 7.2 (KOH) autoclaved Pour the plates and streak autoclaved E.coli cells
18281TOLI AGAR Kan4noIngredients: Agar (Difco) 1.5% CaCl2 0.1% Cycloheximide 2.5 mg per 100 ml Preparation / Remarks: pH 7.2 (KOH) autoclaved w-agar with 2.5 mg of cycloheximide per 100 ml, the antibiotic is filter sterilized and added to the agar just before pouring Cycloheximide does not seem to affect growth of myxobakteria, whereas fungi and amoebae are often inhibited. Combine with 250 mg/L Kanamycin-sulfate (sterilized by filtration) Pour the plates and streak autoclaved E.coli cells
18281VY/2 AGARyesIngredients: Baker's yeast 0.5% (based on fresh weight) CaCl2 0.1% Agar 1.5% Vitamin B12 0.05 % Preparation / Remarks: pH 7.2 autoclaved

Physiology and metabolism

observation

@refobservation
18281No ability for chitin degradation
18281Ability for Yeast lysis
18281Ability for E. coli lysis

Isolation, sampling and environmental information

isolation

  • @ref: 18281
  • sample type: plant residues with little soil
  • sampling date: 1999-09-01
  • geographic location: Bangalore, Karnataka
  • country: India
  • origin.country: IND
  • continent: Asia

isolation source categories

Cat1Cat2Cat3
#Environmental#Terrestrial#Soil
#Host#Plants

External links

@ref: 18281

Reference

@idauthorstitledoi/url
18281Reichenbach, H.Collection description and documentation of myxobacteria by H. Reichenbach, HZI (formerly GBF); Collection curr. located at the DSMZ
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332