Archive BacDiveID:10.13145/bacdive21849.20230509.8.1

Strain identifier

BacDive ID: 21849

Type strain:

Species: Herpetosiphon sp.

Strain Designation: Hp g211

NCBI tax ID(s): 71864 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.

General

@ref: 18281

BacDive-ID: 21849

keywords: Bacteria

description: Herpetosiphon sp. Hp g211 is a bacterium that was isolated from soil with plant residues.

NCBI tax id

NCBI tax id: 71864
Matching level: species

doi: 10.13145/bacdive21849.20230509.8.1

Name and taxonomic classification

LPSN

@ref: 20215
description: domain/bacteria
keyword: phylum/chloroflexota
domain: Bacteria
phylum: Chloroflexota
class: Chloroflexia
order: Herpetosiphonales
family: Herpetosiphonaceae
genus: Herpetosiphon
species: Herpetosiphon sp.
full scientific name: Herpetosiphon Holt and Lewin 1968 (Approved Lists 1980)

@ref: 18281

domain: Bacteria

phylum: Chloroflexi

class: Chloroflexi, not validated

order: Herpetosiphonales

family: Herpetosiphonaceae, not validated

genus: Herpetosiphon

species: Herpetosiphon sp.

full scientific name: Herpetosiphon sp.

strain designation: Hp g211

type strain: no

Culture and growth conditions

culture medium

@ref	name	growth	composition
18281	CHITIN 7 AGAR	yes	Covering agar (thin layer): MgSO4 x 7H2O 0.1% K2HPO4 0.02% Agar 1.5% Preparation / Remarks: pH 7.2 After autoclaving add 30 mL of sterile Chitin suspension (22.5 g/L Chitin in H2O) to 100 mL of the medium and pour on w-agar plates
18281	COLE AGAR	yes	Ingredients: CaCl2 x 2 H2O 1.3g Agar (Difco) 15.0g Distilled water 1000.0ml Preparation / Remarks: pH 7.2 (KOH) autoclaved Pour the plates and streak living E.coli cells
18281	CY-AGAR	yes	Ingredients: Casitone(Difco) 0.3% yeast extract 0.1% CaCl2 0.1% Agar 1.5% Preparation / Remarks: pH 7.2 (KOH) autoclaved
18281	PROBION L AGAR	yes	Ingredients: Probion L 600 (Hoechst) 0.8% MgSO4 x 7H2O 0.1% CaCl2 x 2H2O 0.1% Agar 1.5% Preparation / Remarks: pH 7.2 autoclaved
18281	TOLI AGAR	yes	Ingredients: CaCl2 x 2 H2O1.3g Agar (Difco)15.0g Distilled water1000.0ml Preparation / Remarks: pH 7.2 (KOH) autoclaved Pour the plates and streak autoclaved E.coli cells
18281	TOLI AGAR Kan4	yes	Ingredients: Agar (Difco) 1.5% CaCl2 0.1% Cycloheximide 2.5 mg per 100 ml Preparation / Remarks: pH 7.2 (KOH) autoclaved w-agar with 2.5 mg of cycloheximide per 100 ml, the antibiotic is filter sterilized and added to the agar just before pouring Cycloheximide does not seem to affect growth of myxobakteria, whereas fungi and amoebae are often inhibited. Combine with 250 mg/L Kanamycin-sulfate (sterilized by filtration) Pour the plates and streak autoclaved E.coli cells
18281	VY/2 AGAR	yes	Ingredients: Baker's yeast 0.5% (based on fresh weight) CaCl2 0.1% Agar 1.5% Vitamin B12 0.05 % Preparation / Remarks: pH 7.2 autoclaved

Physiology and metabolism

observation

@ref	observation
18281	Ability for chitin degradation
18281	Ability for protein degradation
18281	Ability for Yeast lysis
18281	Ability for E. coli lysis

antibiotic resistance

@ref	ChEBI	group ID	metabolite	is antibiotic	is resistant	resistance conc.
18281	6104	3	kanamycin	yes	yes	250 µg/mL
18281	27641	3	cycloheximide	yes	yes	25 µg/mL

Isolation, sampling and environmental information

isolation

@ref: 18281
sample type: soil with plant residues
sampling date: 1990-03-01
geographic location: middle highland
country: Ceylon
origin.country: LKA
continent: Asia

isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Terrestrial	#Soil
#Host	#Plants

External links

@ref: 18281

Reference

@id	authors	title	doi/url
18281	Reichenbach, H.	Collection description and documentation of myxobacteria by H. Reichenbach, HZI (formerly GBF); Collection curr. located at the DSMZ
20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.	List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ	10.1099/ijsem.0.004332