Archive BacDiveID:10.13145/bacdive21571.20230509.8.1

Strain identifier

BacDive ID: 21571

Type strain:

Species: Myxococcus stipitatus

Strain Designation: Mx s91

NCBI tax ID(s): 83455 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.

General

@ref: 18281

BacDive-ID: 21571

keywords: Bacteria

description: Myxococcus stipitatus Mx s91 is a bacterium that builds a fruiting body and was isolated from soil.

NCBI tax id

NCBI tax id: 83455
Matching level: species

doi: 10.13145/bacdive21571.20230509.8.1

Name and taxonomic classification

LPSN

@ref: 20215
description: domain/bacteria
keyword: phylum/pseudomonadota
domain: Bacteria
phylum: Pseudomonadota
class: Deltaproteobacteria
order: Myxococcales
family: Myxococcaceae
genus: Myxococcus
species: Myxococcus stipitatus
full scientific name: Myxococcus stipitatus Thaxter 1897 (Approved Lists 1980)

@ref: 18281

domain: Bacteria

phylum: Proteobacteria

class: Deltaproteobacteria

order: Myxococcales

family: Myxococcaceae

genus: Myxococcus

species: Myxococcus stipitatus

full scientific name: Myxococcus stipitatus

strain designation: Mx s91

type strain: no

Morphology

multicellular morphology

@ref: 18281
forms multicellular complex: yes
complex name: fruiting body

Culture and growth conditions

culture medium

@ref	name	growth	composition
18281	CHITIN 6 AGAR	yes	Ingredients: 1. Basal agar: Casitone (Difco) 0.1% Yeast extract (Difco) 0.05% MgSO4 x 7H2O 0.1% Agar 1.2% Preparation / Remarks: pH 7.2 After autoclaving add 30 mL of sterile Chitin suspension (22.5 g/L Chitin in H2O) to 100 mL of the medium 2. Covering agar (thin layer): MgSO4 x 7H2O 0.1% K2HPO4 0.02% Agar 1.5% Preparation / Remarks: pH 7.2 After autoclaving add 30 mL of sterile Chitin suspension (22.5 g/L Chitin in H2O) to 100 mL of the medium
18281	CHITIN 7 AGAR	yes	Covering agar (thin layer): MgSO4 x 7H2O 0.1% K2HPO4 0.02% Agar 1.5% Preparation / Remarks: pH 7.2 After autoclaving add 30 mL of sterile Chitin suspension (22.5 g/L Chitin in H2O) to 100 mL of the medium and pour on w-agar plates
18281	KAN 20 AGAR	no	Ingredients: Agar 1.5% CaCl2 x 2H2O 0.1% MgSO4 x 7H2O 0.1% Yeast extract (Difco) 0.5% Preparation / Remarks: pH 7.2 autoklaved Combine with 10 mg/L Kanamycin-sulfate and 25 mg/L Cycloheximide (each sterilized by filtration)
18281	MD1 liquid medium	yes	Ingredients: Casitone 0.3% CaCl2 0.05% MgSO4 x 7H2O 0.2% Vit. B12 0.5 g/ml Trace element-solution 0.5 ml/100 ml
18281	Myx MEDIUM	yes	Ingredients: Peptone (Difco) 1% Glucose 1% Yeast extract (Difco) 0.1% Phosphate buffer 0.01 M pH 6.5 Aerobacter aerogenes (sterilized with formalin) 500 mg/L (dry weight) as described by Kerr, N.S. (1963) The Growth of Myxamoebae of the True Slime Mould, Didymium nigripes, in Axenic Culture. J Gen Microbiol 32: 409-416.
18281	TOLI AGAR	yes	Ingredients: CaCl2 x 2 H2O1.3g Agar (Difco)15.0g Distilled water1000.0ml Preparation / Remarks: pH 7.2 (KOH) autoclaved Pour the plates and streak autoclaved E.coli cells
18281	VY/2 AGAR	yes	Ingredients: Baker's yeast 0.5% (based on fresh weight) CaCl2 0.1% Agar 1.5% Vitamin B12 0.05 % Preparation / Remarks: pH 7.2 autoclaved

Physiology and metabolism

observation

@ref	observation
18281	Strong ability of chitin decomposition
18281	Ability for chitin degradation
18281	Ability for Yeast lysis

Isolation, sampling and environmental information

isolation

@ref: 18281
sample type: soil
sampling date: 1999-08-01
geographic location: near Braunschweig
country: Germany
origin.country: DEU
continent: Europe

isolation source categories

Cat1: #Environmental
Cat2: #Terrestrial
Cat3: #Soil

External links

@ref: 18281

Reference

@id	authors	title	doi/url
18281	Reichenbach, H.	Collection description and documentation of myxobacteria by H. Reichenbach, HZI (formerly GBF); Collection curr. located at the DSMZ
20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.	List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ	10.1099/ijsem.0.004332