Strain identifier

BacDive ID: 21155

Type strain: No

Species: Cystobacter sp.

Strain Designation: Cb o5

NCBI tax ID(s): 1965334 (species)

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General

@ref: 18281

BacDive-ID: 21155

keywords: Bacteria

description: Cystobacter sp. Cb o5 is a bacterium that builds a fruiting body and was isolated from soil with plant residues.

NCBI tax id

  • NCBI tax id: 1965334
  • Matching level: species

doi: 10.13145/bacdive21155.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Deltaproteobacteria
  • order: Myxococcales
  • family: Archangiaceae
  • genus: Cystobacter
  • species: Cystobacter sp.
  • full scientific name: Cystobacter Schroeter 1886 (Approved Lists 1980)

@ref: 18281

domain: Bacteria

phylum: Proteobacteria

class: Deltaproteobacteria

order: Myxococcales

family: Cystobacteraceae

genus: Cystobacter

species: Cystobacter sp.

full scientific name: Cystobacter sp.

strain designation: Cb o5

type strain: no

Morphology

multicellular morphology

  • @ref: 18281
  • forms multicellular complex: yes
  • complex name: fruiting body

multimedia

@refmultimedia contentcaptionintellectual property rights
18281/dsmz_profile/Pictures_DSMZ_Web/Cystobacter_ova-ranarum/Cb_o5Cb o5Helmholtz-Zentrum für Infektionsforschung GmbH
18281/dsmz_profile/Pictures_DSMZ_Web/Cystobacter_ova-ranarum/Cb_o5Cb o5Helmholtz-Zentrum für Infektionsforschung GmbH
18281/dsmz_profile/Pictures_DSMZ_Web/Cystobacter_ova-ranarum/Cb_o5Cb o5Helmholtz-Zentrum für Infektionsforschung GmbH
18281/dsmz_profile/Pictures_DSMZ_Web/Cystobacter_ova-ranarum/Cb_o5Cb o5Helmholtz-Zentrum für Infektionsforschung GmbH
18281/dsmz_profile/Pictures_DSMZ_Web/Cystobacter_ova-ranarum/Cb_o5Cb o5Helmholtz-Zentrum für Infektionsforschung GmbH
18281/dsmz_profile/Pictures_DSMZ_Web/Cystobacter_ova-ranarum/Cb_o5Cb o5Helmholtz-Zentrum für Infektionsforschung GmbH
18281/dsmz_profile/Pictures_DSMZ_Web/Cystobacter_ova-ranarum/Cb_o5Cb o5Helmholtz-Zentrum für Infektionsforschung GmbH
18281/dsmz_profile/Pictures_DSMZ_Web/Cystobacter_ova-ranarum/Cb_o5Cb o5Helmholtz-Zentrum für Infektionsforschung GmbH
18281/dsmz_profile/Pictures_DSMZ_Web/Cystobacter_ova-ranarum/Cb_o5Cb o5Helmholtz-Zentrum für Infektionsforschung GmbH
18281/dsmz_profile/Pictures_DSMZ_Web/Cystobacter_ova-ranarum/Cb_o5Cb o5Helmholtz-Zentrum für Infektionsforschung GmbH

Culture and growth conditions

culture medium

@refnamegrowthcomposition
18281CHITIN 6 AGARyesIngredients: 1. Basal agar: Casitone (Difco) 0.1% Yeast extract (Difco) 0.05% MgSO4 x 7H2O 0.1% Agar 1.2% Preparation / Remarks: pH 7.2 After autoclaving add 30 mL of sterile Chitin suspension (22.5 g/L Chitin in H2O) to 100 mL of the medium 2. Covering agar (thin layer): MgSO4 x 7H2O 0.1% K2HPO4 0.02% Agar 1.5% Preparation / Remarks: pH 7.2 After autoclaving add 30 mL of sterile Chitin suspension (22.5 g/L Chitin in H2O) to 100 mL of the medium
18281CHITIN 7 AGARyesCovering agar (thin layer): MgSO4 x 7H2O 0.1% K2HPO4 0.02% Agar 1.5% Preparation / Remarks: pH 7.2 After autoclaving add 30 mL of sterile Chitin suspension (22.5 g/L Chitin in H2O) to 100 mL of the medium and pour on w-agar plates
18281COLE AGARyesIngredients: CaCl2 x 2 H2O 1.3g Agar (Difco) 15.0g Distilled water 1000.0ml Preparation / Remarks: pH 7.2 (KOH) autoclaved Pour the plates and streak living E.coli cells
18281KAN 14 AGARyesIngredients: Baker's yeast 0.5% (based on fresh weight) CaCl2 0.1% Agar 1.5% Vitamin B12 0.05 % Preparation / Remarks: pH 7.2 autoclaved Combine with 1 mg/L Kanamycin-sulfate (sterilized by filtration)
18281KAN 17 AGARyesIngredients: Baker's yeast 0.5% (based on fresh weight) CaCl2 0.1% Agar 1.5% Vitamine B12 0.5 µg/ml Preparation / Remarks: pH 7.2 autoclaved Combine with 1 g/L Kanamycin-sulfate and 0.1% MgSO4 x 7H2O (sterilized by filtration)
18281Myx MEDIUMyesIngredients: Peptone (Difco) 1% Glucose 1% Yeast extract (Difco) 0.1% Phosphate buffer 0.01 M pH 6.5 Aerobacter aerogenes (sterilized with formalin) 500 mg/L (dry weight) as described by Kerr, N.S. (1963) The Growth of Myxamoebae of the True Slime Mould, Didymium nigripes, in Axenic Culture. J Gen Microbiol 32: 409-416.
18281TOLI AGARyesIngredients: CaCl2 x 2 H2O1.3g Agar (Difco)15.0g Distilled water1000.0ml Preparation / Remarks: pH 7.2 (KOH) autoclaved Pour the plates and streak autoclaved E.coli cells
18281VY/2 AGARyesIngredients: Baker's yeast 0.5% (based on fresh weight) CaCl2 0.1% Agar 1.5% Vitamin B12 0.05 % Preparation / Remarks: pH 7.2 autoclaved

Physiology and metabolism

observation

@refobservation
18281No ability for chitin degradation
18281Ability for Yeast lysis
18281Ability for E. coli lysis

antibiotic resistance

@refChEBImetaboliteis antibioticis resistantresistance conc.
182816104kanamycinyesyes1 µg/mL
182816104kanamycinyesyes1000 µg/mL

Isolation, sampling and environmental information

isolation

  • @ref: 18281
  • sample type: soil with plant residues
  • sampling date: 1999-09-01
  • geographic location: Bangalore, Karnataka
  • country: India
  • origin.country: IND
  • continent: Asia

isolation source categories

Cat1Cat2Cat3
#Environmental#Terrestrial#Soil
#Host#Plants

External links

@ref: 18281

Reference

@idauthorstitledoi/url
18281Reichenbach, H.Collection description and documentation of myxobacteria by H. Reichenbach, HZI (formerly GBF); Collection curr. located at the DSMZ
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332