Strain identifier

BacDive ID: 211

Type strain: No

Species: Trueperella pyogenes

Strain Designation: 3329

Strain history: <- K.H. Schleifer <- CCM <- W. Kötsche, 3329

NCBI tax ID(s): 1661 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 8942

BacDive-ID: 211

DSM-Number: 20594

keywords: Bacteria, anaerobe, human pathogen

description: Trueperella pyogenes 3329 is an anaerobe human pathogen that was isolated from mammary gland of a cow with pyogenic mastitis.

NCBI tax id

  • NCBI tax id: 1661
  • Matching level: species

strain history

  • @ref: 8942
  • history: <- K.H. Schleifer <- CCM <- W. Kötsche, 3329

doi: 10.13145/bacdive211.20240510.9

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Actinomycetales
  • family: Actinomycetaceae
  • genus: Trueperella
  • species: Trueperella pyogenes
  • full scientific name: Trueperella pyogenes (Glage 1903) Yassin et al. 2011
  • synonyms

    @refsynonym
    20215Actinomyces pyogenes
    20215Arcanobacterium pyogenes
    20215Corynebacterium pyogenes
    20215Bacillus pyogenes

@ref: 8942

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Actinomycetaceae

genus: Trueperella

species: Trueperella pyogenes

full scientific name: Trueperella pyogenes (Glage 1903) Yassin et al. 2011

strain designation: 3329

type strain: no

Culture and growth conditions

culture medium

  • @ref: 8942
  • name: PYG MEDIUM (MODIFIED) (DSMZ Medium 104)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/104
  • composition: Name: PYG MEDIUM (modified) (DSMZ Medium 104) Composition: Yeast extract 10.0 g/l Peptone 5.0 g/l Trypticase peptone 5.0 g/l Beef extract 5.0 g/l Glucose 5.0 g/l L-Cysteine HCl x H2O 0.5 g/l NaHCO3 0.4 g/l NaCl 0.08 g/l K2HPO4 0.04 g/l KH2PO4 0.04 g/l MgSO4 x 7 H2O 0.02 g/l CaCl2 x 2 H2O 0.01 g/l Hemin 0.005 g/l Ethanol 0.0038 g/l Resazurin 0.001 g/l Tween 80 Vitamin K1 NaOH Distilled water

culture temp

  • @ref: 8942
  • growth: positive
  • type: growth
  • temperature: 37

Physiology and metabolism

oxygen tolerance

  • @ref: 8942
  • oxygen tolerance: anaerobe

murein

  • @ref: 8942
  • murein short key: A11.53
  • type: A5alpha L-Lys-L-Ala-L-Lys-D-Glu

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6838016199urea-hydrolysis
6838029016arginine-hydrolysis
6838029985L-glutamate-degradation
6838017632nitrate-reduction
6838027897tryptophan-energy source

metabolite production

  • @ref: 68380
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 68380
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
68380serine arylamidase+
68380glutamyl-glutamate arylamidase-
68380glycin arylamidase+
68380alanine arylamidase+3.4.11.2
68380tyrosine arylamidase-
68380phenylalanine arylamidase-
68380proline-arylamidase+3.4.11.5
68380L-arginine arylamidase+
68380alkaline phosphatase-3.1.3.1
68380tryptophan deaminase-4.1.99.1
68380alpha-fucosidase-3.2.1.51
68380glutamate decarboxylase-4.1.1.15
68380beta-glucuronidase+3.2.1.31
68380alpha-arabinosidase-3.2.1.55
68380beta-glucosidase-3.2.1.21
68380alpha-glucosidase+3.2.1.20
68380beta-Galactosidase 6-phosphate-
68380beta-galactosidase+3.2.1.23
68380alpha-galactosidase-3.2.1.22
68380arginine dihydrolase-3.5.3.6
68380urease-3.5.1.5

API rID32A

@refUREADH Argalpha GALbeta GALbeta GPalpha GLUbeta GLUalpha ARAbeta GURbeta NAGMNERAFGDCalpha FUCNITINDPALArgAProALGAPheALeuAPyrATyrAAlaAGlyAHisAGGASerA
8942---+-+--++/-++/------+++/--+/-+/--++--+
8942---+-+--++/--------++--+/-+/--+++/--+

Isolation, sampling and environmental information

isolation

  • @ref: 8942
  • sample type: mammary gland of a cow with pyogenic mastitis

isolation source categories

Cat1Cat2Cat3
#Infection#Disease
#Host#Mammals#Bovinae (Cow, Cattle)
#Host Body-Site#Organ#Mammary gland

Safety information

risk assessment

  • @ref: 8942
  • pathogenicity human: yes
  • pathogenicity animal: yes
  • biosafety level: 2
  • biosafety level comment: Risk group (German classification)

External links

@ref: 8942

culture collection no.: DSM 20594, CCM 5753

straininfo link

  • @ref: 69894
  • straininfo: 49653

literature

  • topic: Phylogeny
  • Pubmed-ID: 26494240
  • title: Bovine vaginal strain Kocuria kristinae and its characterization.
  • authors: Stykova E, Nemcova R, Gancarcikova S, Valocky I, Laukova A
  • journal: Folia Microbiol (Praha)
  • DOI: 10.1007/s12223-015-0431-x
  • year: 2015
  • mesh: Animals, Anti-Bacterial Agents/pharmacology, Bacterial Adhesion, Cattle, Female, Gram-Positive Bacterial Infections/*veterinary, Microbial Sensitivity Tests, Micrococcaceae/*classification/drug effects/isolation & purification, Phenotype, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Vagina/*microbiology
  • topic2: Phenotype

Reference

@idauthorscataloguedoi/urltitle
8942Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 20594)https://www.dsmz.de/collection/catalogue/details/culture/DSM-20594
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
68380Automatically annotated from API rID32A
69894Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID49653.1StrainInfo: A central database for resolving microbial strain identifiers