Strain identifier

BacDive ID: 2088

Type strain: Yes

Species: Ralstonia mannitolilytica

Strain history: CIP <- 2001, LMG <- 1985, NCIMB <- 1972, I. Phillips, St Thomas'Hosp., London, UK

NCBI tax ID(s): 105219 (species)

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General

@ref: 7032

BacDive-ID: 2088

DSM-Number: 17512

keywords: genome sequence, 16S sequence, Bacteria, obligate aerobe, mesophilic, Gram-negative, rod-shaped

description: Ralstonia mannitolilytica DSM 17512 is an obligate aerobe, mesophilic, Gram-negative bacterium that was isolated from blood.

NCBI tax id

  • NCBI tax id: 105219
  • Matching level: species

strain history

@refhistory
7032<- M. Vaneechoutte, University of Ghent
67770LMG 6866 <-- NCIMB 10805 <-- I. Phillips (St. Thomas' Hosp., London).
120780CIP <- 2001, LMG <- 1985, NCIMB <- 1972, I. Phillips, St Thomas'Hosp., London, UK

doi: 10.13145/bacdive2088.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Betaproteobacteria
  • order: Burkholderiales
  • family: Burkholderiaceae
  • genus: Ralstonia
  • species: Ralstonia mannitolilytica
  • full scientific name: Ralstonia mannitolilytica corrig. De Baere et al. 2001

  • synonyms

    • @ref: 20215
    • synonym: Ralstonia mannitolytica

@ref: 7032

domain: Bacteria

phylum: Proteobacteria

class: Betaproteobacteria

order: Burkholderiales

family: Burkholderiaceae

genus: Ralstonia

species: Ralstonia mannitolilytica

full scientific name: Ralstonia mannitolilytica De Baere et al. 2001

type strain: yes

Morphology

cell morphology

  • @ref: 120780
  • gram stain: negative
  • cell shape: rod-shaped
  • motility: no

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
7032R2A MEDIUM (DSMZ Medium 830)yeshttps://mediadive.dsmz.de/medium/830Name: R2A MEDIUM (DSMZ Medium 830) Composition: Agar 15.0 g/l Casamino acids 0.5 g/l Starch 0.5 g/l Glucose 0.5 g/l Proteose peptone 0.5 g/l Yeast extract 0.5 g/l K2HPO4 0.3 g/l Na-pyruvate 0.3 g/l MgSO4 x 7 H2O 0.05 g/l Distilled water
7032NUTRIENT AGAR (DSMZ Medium 1)yeshttps://mediadive.dsmz.de/medium/1Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water
33103MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
120780CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
7032positivegrowth28mesophilic
33103positivegrowth30mesophilic
67770positivegrowth30mesophilic
120780positivegrowth25-41
120780nogrowth5psychrophilic
120780nogrowth10psychrophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 120780
  • oxygen tolerance: obligate aerobe

halophily

@refsaltgrowthtested relationconcentration
120780NaClpositivegrowth0-2 %
120780NaClnogrowth4 %
120780NaClnogrowth6 %
120780NaClnogrowth8 %
120780NaClnogrowth10 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
12078016947citrate+carbon source
1207804853esculin-hydrolysis
12078017632nitrate-reduction
12078016301nitrite-reduction
12078017632nitrate-respiration

antibiotic resistance

  • @ref: 120780
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

  • @ref: 120780
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase+
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)+
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase+3.1.3.1
120780oxidase+
120780beta-galactosidase-3.2.1.23
120780alcohol dehydrogenase-1.1.1.1
120780gelatinase+/-
120780amylase-
120780DNase-
120780caseinase+3.4.21.50
120780catalase+1.11.1.6
120780tween esterase+
120780lecithinase-
120780lipase-
120780lysine decarboxylase-4.1.1.18
120780ornithine decarboxylase-4.1.1.17
120780protease+
120780tryptophan deaminase-
120780urease+3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
120780-++++++---++--------

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
120780+++----------------+++----+----+-+---------++--+-+++-++++-+++-++---+-----+-++++++-+---+-+++++++++++

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentisolation date
7032bloodLondonUnited KingdomGBREurope
67770Blood
120780Contaminated autoclave fluidsLondonUnited KingdomGBREurope1971

isolation source categories

Cat1Cat2Cat3
#Host Body Product#Fluids#Blood
#Infection#Patient

taxonmaps

  • @ref: 69479
  • File name: preview.99_921.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_12;96_51;97_54;98_59;99_921&stattab=map
  • Last taxonomy: Ralstonia pickettii
  • 16S sequence: AJ270258
  • Sequence Identity:
  • Total samples: 8327
  • soil counts: 338
  • aquatic counts: 1121
  • animal counts: 4145
  • plant counts: 2723

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
70322Risk group (German classification)
1207801Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Ralstonia mannitolilytica strain CIP 107281 16S ribosomal RNA gene, partial sequenceEU0241521316ena105219
20218Ralstonia thomasii 16S rRNA gene, strain LMG6866AJ2702581449ena105219

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Ralstonia mannitolilytica LMG 6866GCA_905397375contigncbi105219
66792Ralstonia mannitolilytica strain LMG 6866105219.54wgspatric105219

GC content

  • @ref: 67770
  • GC-content: 66.2
  • method: high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileyes90.182no
flagellatedno50no
gram-positiveno98.46no
anaerobicno99.284no
aerobicyes95.056no
halophileno94.202no
spore-formingno93.619no
thermophileno97.755no
glucose-utilyes75.988no
glucose-fermentno91.581no

External links

@ref: 7032

culture collection no.: DSM 17512, LMG 6866, JCM 11284, ATCC BAA 716, CCUG 38408, CCUG 45027, CIP 107281, NCIMB 10805

straininfo link

  • @ref: 71735
  • straininfo: 13771

literature

  • topic: Phylogeny
  • Pubmed-ID: 11321101
  • title: Classification of Ralstonia pickettii biovar 3/'thomasii' strains (Pickett 1994) and of new isolates related to nosocomial recurrent meningitis as Ralstonia mannitolytica sp. nov.
  • authors: De Baere T, Steyaert S, Wauters G, Des Vos P, Goris J, Coenye T, Suyama T, Verschraegen G, Vaneechoutte M
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/00207713-51-2-547
  • year: 2001
  • mesh: Base Composition, Betaproteobacteria/*classification/genetics/*pathogenicity, Cross Infection/*microbiology, Fatty Acids/analysis, Humans, Meningitis, Bacterial/epidemiology/*microbiology, Molecular Sequence Data, Recurrence, Sequence Analysis, DNA, Sequence Homology, Nucleic Acid, Terminology as Topic
  • topic2: Pathogenicity

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
7032Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 17512)https://www.dsmz.de/collection/catalogue/details/culture/DSM-17512
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
33103Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/4699
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
71735Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID13771.1StrainInfo: A central database for resolving microbial strain identifiers
120780Curators of the CIPCollection of Institut Pasteur (CIP 107281)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20107281