Strain identifier

BacDive ID: 20574

Type strain: No

Species: Archangium sp.

Strain Designation: Ar D76

NCBI tax ID(s): 1872627 (species)

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General

@ref: 18281

BacDive-ID: 20574

keywords: Bacteria

description: Archangium sp. Ar D76 is a bacterium that builds a fruiting body and was isolated from soil.

NCBI tax id

  • NCBI tax id: 1872627
  • Matching level: species

doi: 10.13145/bacdive20574.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Deltaproteobacteria
  • order: Myxococcales
  • family: Archangiaceae
  • genus: Archangium
  • species: Archangium sp.
  • full scientific name: Archangium Jahn 1924 (Approved Lists 1980)
  • synonyms

    • @ref: 20215
    • synonym: Angiococcus

@ref: 18281

domain: Bacteria

phylum: Proteobacteria

class: Deltaproteobacteria

order: Myxococcales

family: Cystobacteraceae

genus: Archangium

species: Archangium sp.

full scientific name: Archangium sp.

strain designation: Ar D76

type strain: no

Morphology

multicellular morphology

  • @ref: 18281
  • forms multicellular complex: yes
  • complex name: fruiting body

Culture and growth conditions

culture medium

@refnamegrowthcomposition
18281CA2 AGARyesIngredients: Stock solution 1 (in 100ml): KNO3 7.5g K2HPO4 7.5g Stock solution 2 (in 100ml): MgSO4 x 7H2O 1.5g Stock solution 3 (in 100ml): CaCl2 0.2g FeCl3 0.15g Basal medium: Agar 1.5% Preparation / Remarks: after autoclaving to: a) 1% (v/v) of the 3 stock solutions b) 1% glucose c) trace elements
18281CHITIN 6 AGARyesIngredients: 1. Basal agar: Casitone (Difco) 0.1% Yeast extract (Difco) 0.05% MgSO4 x 7H2O 0.1% Agar 1.2% Preparation / Remarks: pH 7.2 After autoclaving add 30 mL of sterile Chitin suspension (22.5 g/L Chitin in H2O) to 100 mL of the medium 2. Covering agar (thin layer): MgSO4 x 7H2O 0.1% K2HPO4 0.02% Agar 1.5% Preparation / Remarks: pH 7.2 After autoclaving add 30 mL of sterile Chitin suspension (22.5 g/L Chitin in H2O) to 100 mL of the medium
18281CHITIN 7 AGARyesCovering agar (thin layer): MgSO4 x 7H2O 0.1% K2HPO4 0.02% Agar 1.5% Preparation / Remarks: pH 7.2 After autoclaving add 30 mL of sterile Chitin suspension (22.5 g/L Chitin in H2O) to 100 mL of the medium and pour on w-agar plates
18281COLE AGARyesIngredients: CaCl2 x 2 H2O 1.3g Agar (Difco) 15.0g Distilled water 1000.0ml Preparation / Remarks: pH 7.2 (KOH) autoclaved Pour the plates and streak living E.coli cells
18281HP6 AGARyesIngredients: Na-glutaminate 1% MgSO4x7H2O 0.1% Yeast extract (Difco) 0.1% Vitamin B12 0.5 g/ml Glucose 0.5% Agar 1.5% Preparation / Remarks: pH 7.2 autoclaved Autoclaving glucose separately
18281Myx MEDIUMyesIngredients: Peptone (Difco) 1% Glucose 1% Yeast extract (Difco) 0.1% Phosphate buffer 0.01 M pH 6.5 Aerobacter aerogenes (sterilized with formalin) 500 mg/L (dry weight) as described by Kerr, N.S. (1963) The Growth of Myxamoebae of the True Slime Mould, Didymium nigripes, in Axenic Culture. J Gen Microbiol 32: 409-416.
18281PROBION L AGARyesIngredients: Probion L 600 (Hoechst) 0.8% MgSO4 x 7H2O 0.1% CaCl2 x 2H2O 0.1% Agar 1.5% Preparation / Remarks: pH 7.2 autoclaved
18281SKIM MILK 2 AGARyesIngredients: cy-Agar 300 ml Skim milk (Oxoid) 15g H2O 60 ml Preparation / Remarks: 300 ml cy-agar autoclaved. Separately 15 g skim milk (Oxoid) in 60 ml water and exactly 15 min autoclaving at 115 °C. Mix the two and pour a thin layer on CA2-plates.
18281TOLI AGARyesIngredients: CaCl2 x 2 H2O1.3g Agar (Difco)15.0g Distilled water1000.0ml Preparation / Remarks: pH 7.2 (KOH) autoclaved Pour the plates and streak autoclaved E.coli cells
18281VY/2 AGARyesIngredients: Baker's yeast 0.5% (based on fresh weight) CaCl2 0.1% Agar 1.5% Vitamin B12 0.05 % Preparation / Remarks: pH 7.2 autoclaved
18281XYLAN 2 AGARyesIngredients: Xylan 0.5% (NH4)2SO4 0.1% Agar 1% Preparation / Remarks: pH 7.2 Xylan (Serva pure molecular weight 25000) Pour after autoclaving as a thin layer on standard cy-agar
18281XYLAN 3 AGARyesIngredients: Xylan 0.5% (NH4)2SO4 0.05% Agar 1.5% Preparation / Remarks: pH 7.2 Pour after autoclaving as a thin cover layer on CyG2 Grundagar Xylan (Serva pure molecular weight ~ 25000)

Physiology and metabolism

observation

@refobservation
18281Strong ability to Yeast lysis
18281Ability for casein degradation
18281Ability for chitin degradation
18281Ability for protein degradation
18281Ability for Yeast lysis
18281Ability for E. coli lysis

Isolation, sampling and environmental information

isolation

  • @ref: 18281
  • sample type: soil
  • country: Benin
  • origin.country: BEN
  • continent: Africa

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Terrestrial
  • Cat3: #Soil

External links

@ref: 18281

Reference

@idauthorstitledoi/url
18281Reichenbach, H.Collection description and documentation of myxobacteria by H. Reichenbach, HZI (formerly GBF); Collection curr. located at the DSMZ
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332