Strain identifier

BacDive ID: 20412

Type strain: No

Species: Sorangium cellulosum

Strain Designation: So ce614

NCBI tax ID(s): 56 (species)

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General

@ref: 18281

BacDive-ID: 20412

keywords: Bacteria

description: Sorangium cellulosum So ce614 is a bacterium that builds a fruiting body and was isolated from soil from tropical rainforest.

NCBI tax id

  • NCBI tax id: 56
  • Matching level: species

doi: 10.13145/bacdive20412.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Deltaproteobacteria
  • order: Myxococcales
  • family: Polyangiaceae
  • genus: Sorangium
  • species: Sorangium cellulosum
  • full scientific name: Sorangium cellulosum (Brockman 1989 ex Imshenetski and Solntseva 1936) Reichenbach 2007
  • synonyms

    • @ref: 20215
    • synonym: Polyangium cellulosum

@ref: 18281

domain: Bacteria

phylum: Proteobacteria

class: Deltaproteobacteria

order: Myxococcales

family: Sandaracinaceae

genus: Sorangium

species: Sorangium cellulosum

full scientific name: Sorangium cellulosum

strain designation: So ce614

type strain: no

Morphology

multicellular morphology

  • @ref: 18281
  • forms multicellular complex: yes
  • complex name: fruiting body

Culture and growth conditions

culture medium

@refnamegrowthcomposition
18281CA13 AGARyesIngredients: Stock solution 1 (in 100ml): (NH4)2SO4 0.5g K2HPO4 7.5g Stock solution 2 (in 100ml): MgSO4 x 7H2O 1.5g Stock solution 3 (in 100ml): CaCl2 0.2g FeCl3 0.15g Basal medium: Agar 1.5% Preparation / Remarks: after autoclaving to: a) 1% (v/v) of the 3 stock solutions b) 1% glucose c) trace elements
18281CA2 AGARyesIngredients: Stock solution 1 (in 100ml): KNO3 7.5g K2HPO4 7.5g Stock solution 2 (in 100ml): MgSO4 x 7H2O 1.5g Stock solution 3 (in 100ml): CaCl2 0.2g FeCl3 0.15g Basal medium: Agar 1.5% Preparation / Remarks: after autoclaving to: a) 1% (v/v) of the 3 stock solutions b) 1% glucose c) trace elements
18281CHITIN 7 AGARyesCovering agar (thin layer): MgSO4 x 7H2O 0.1% K2HPO4 0.02% Agar 1.5% Preparation / Remarks: pH 7.2 After autoclaving add 30 mL of sterile Chitin suspension (22.5 g/L Chitin in H2O) to 100 mL of the medium and pour on w-agar plates
18281KAN 5 AGARyesIngredients: Baker's yeast 0.5% (based on fresh weight) CaCl2 0.1% Agar 1.5% Vitamine B12 0.5 µg/ml Preparation / Remarks: pH 7.2 autoclaved Combine with 250 mg/L Kanamycin-sulfate (sterilized by filtration)
18281SKIM MILK 1 AGARyesIngredients: cy-Agar 300 ml Skim milk (Oxoid) 15g H2O 60 ml Preparation / Remarks: 300 ml cy-agar autoclaved. Separately 15 g skim milk (Oxoid) in 60 ml water and exactly 15 min autoclaving at 115 °C. Mix the two and pour a thin layer on cy-plates.
18281STAN 21 AGAR with FilteryesIngredients: 1. Agar 1% K2HPO4 0.1% Yeast extract (Difco) 0.002% 2. (NH4)2SO4 0.1% MgSO4 x 7H2O 0.1% CaCl2 x 2H2O 0.1% MnSO4 x H2O 0.01% FeCl3 x 6H2O 0.02% MnSO4 x 7H2O 0.01% FeCl3 0.02% Traceelement-solution 1ml to 100 ml Preparation / Remarks: 1. in 2/3 of the volume after autoclaving 1 + 2 together and pour plates. Hang up a sterile-filter paper
18281VY/2 AGARyesIngredients: Baker's yeast 0.5% (based on fresh weight) CaCl2 0.1% Agar 1.5% Vitamin B12 0.05 % Preparation / Remarks: pH 7.2 autoclaved

Physiology and metabolism

observation

@refobservation
18281Strong ability to Yeast lysis
18281Strong ability of chitin decomposition
18281Ability for casein degradation
18281Ability for chitin degradation
18281Ability for protein degradation
18281No ability for Yeast lysis
18281Ability for Yeast lysis

antibiotic resistance

  • @ref: 18281
  • ChEBI: 6104
  • metabolite: kanamycin
  • is antibiotic: yes
  • is resistant: yes
  • resistance conc.: 250 µg/mL

Isolation, sampling and environmental information

isolation

  • @ref: 18281
  • sample type: soil from tropical rainforest
  • sampling date: 1990-03-01
  • geographic location: southern part of Ceylon
  • country: Ceylon
  • origin.country: LKA
  • continent: Asia

isolation source categories

Cat1Cat2Cat3
#Environmental#Terrestrial#Forest
#Environmental#Terrestrial#Soil
#Climate#Hot#Tropical

External links

@ref: 18281

Reference

@idauthorstitledoi/url
18281Reichenbach, H.Collection description and documentation of myxobacteria by H. Reichenbach, HZI (formerly GBF); Collection curr. located at the DSMZ
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332