Strain identifier

BacDive ID: 20311

Type strain: No

Species: Sporocytophaga myxococcoides

Strain Designation: Sp m80

NCBI tax ID(s): 153721 (species)

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General

@ref: 18281

BacDive-ID: 20311

keywords: Bacteria

description: Sporocytophaga myxococcoides Sp m80 is a bacterium that was isolated from soil with plant residues from tropical rain forest.

NCBI tax id

  • NCBI tax id: 153721
  • Matching level: species

doi: 10.13145/bacdive20311.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacteroidota
  • domain: Bacteria
  • phylum: Bacteroidota
  • class: Cytophagia
  • order: Cytophagales
  • family: Cytophagaceae
  • genus: Sporocytophaga
  • species: Sporocytophaga myxococcoides
  • full scientific name: Sporocytophaga myxococcoides (Krzemieniewska 1933) Stanier 1940 (Approved Lists 1980)

  • synonyms

    • @ref: 20215
    • synonym: Cytophaga myxococcoides

@ref: 18281

domain: Bacteria

phylum: Bacteroidetes, not validated

class: Cytophagia

order: Cytophagales

family: Cytophagacea

genus: Sporocytophaga

species: Sporocytophaga myxococcoides

full scientific name: Sporocytophaga myxococcoides

strain designation: Sp m80

type strain: no

Culture and growth conditions

culture medium

@refnamegrowthcomposition
18281CA2 AGARyesIngredients: Stock solution 1 (in 100ml): KNO3 7.5g K2HPO4 7.5g Stock solution 2 (in 100ml): MgSO4 x 7H2O 1.5g Stock solution 3 (in 100ml): CaCl2 0.2g FeCl3 0.15g Basal medium: Agar 1.5% Preparation / Remarks: after autoclaving to: a) 1% (v/v) of the 3 stock solutions b) 1% glucose c) trace elements
18281CHITIN 6 AGARyesIngredients: 1. Basal agar: Casitone (Difco) 0.1% Yeast extract (Difco) 0.05% MgSO4 x 7H2O 0.1% Agar 1.2% Preparation / Remarks: pH 7.2 After autoclaving add 30 mL of sterile Chitin suspension (22.5 g/L Chitin in H2O) to 100 mL of the medium 2. Covering agar (thin layer): MgSO4 x 7H2O 0.1% K2HPO4 0.02% Agar 1.5% Preparation / Remarks: pH 7.2 After autoclaving add 30 mL of sterile Chitin suspension (22.5 g/L Chitin in H2O) to 100 mL of the medium
18281CHITIN 7 AGARnoCovering agar (thin layer): MgSO4 x 7H2O 0.1% K2HPO4 0.02% Agar 1.5% Preparation / Remarks: pH 7.2 After autoclaving add 30 mL of sterile Chitin suspension (22.5 g/L Chitin in H2O) to 100 mL of the medium and pour on w-agar plates
18281CY-AGARyesIngredients: Casitone(Difco) 0.3% yeast extract 0.1% CaCl2 0.1% Agar 1.5% Preparation / Remarks: pH 7.2 (KOH) autoclaved
18281Myx MEDIUMnoIngredients: Peptone (Difco) 1% Glucose 1% Yeast extract (Difco) 0.1% Phosphate buffer 0.01 M pH 6.5 Aerobacter aerogenes (sterilized with formalin) 500 mg/L (dry weight) as described by Kerr, N.S. (1963) The Growth of Myxamoebae of the True Slime Mould, Didymium nigripes, in Axenic Culture. J Gen Microbiol 32: 409-416.
18281SKIM MILK 2 AGARyesIngredients: cy-Agar 300 ml Skim milk (Oxoid) 15g H2O 60 ml Preparation / Remarks: 300 ml cy-agar autoclaved. Separately 15 g skim milk (Oxoid) in 60 ml water and exactly 15 min autoclaving at 115 °C. Mix the two and pour a thin layer on CA2-plates.
18281SKIM MILK 3 AGARyesIngredients: cy-Agar 300 ml Skim milk (Oxoid) 15g H2O 60 ml Preparation / Remarks: 300 ml cy-agar autoclaved. Separately 15 g skim milk (Oxoid) in 60 ml water and exactly 15 min autoclaving at 115 °C. Mix the two and pour a thin layer on CYG2-plates.
18281STAN 21 AGAR with FilteryesIngredients: 1. Agar 1% K2HPO4 0.1% Yeast extract (Difco) 0.002% 2. (NH4)2SO4 0.1% MgSO4 x 7H2O 0.1% CaCl2 x 2H2O 0.1% MnSO4 x H2O 0.01% FeCl3 x 6H2O 0.02% MnSO4 x 7H2O 0.01% FeCl3 0.02% Traceelement-solution 1ml to 100 ml Preparation / Remarks: 1. in 2/3 of the volume after autoclaving 1 + 2 together and pour plates. Hang up a sterile-filter paper
18281XYLAN 3 AGARyesIngredients: Xylan 0.5% (NH4)2SO4 0.05% Agar 1.5% Preparation / Remarks: pH 7.2 Pour after autoclaving as a thin cover layer on CyG2 Grundagar Xylan (Serva pure molecular weight ~ 25000)

Physiology and metabolism

observation

@refobservation
18281Ability for casein degradation
18281No ability for chitin degradation

Isolation, sampling and environmental information

isolation

  • @ref: 18281
  • sample type: soil with plant residues from tropical rain forest
  • sampling date: 1988-03-01
  • geographic location: near Parana
  • country: Brazil
  • origin.country: BRA
  • continent: Middle and South America

isolation source categories

Cat1Cat2Cat3
#Environmental#Terrestrial#Forest
#Environmental#Terrestrial#Soil
#Host#Plants
#Climate#Hot#Tropical

External links

@ref: 18281

Reference

@idauthorstitledoi/urljournalpubmed
18281Reichenbach, H.Collection description and documentation of myxobacteria by H. Reichenbach, HZI (formerly GBF); Collection curr. located at the DSMZ
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar SchomburgBRENDA, the ELIXIR core data resource in 2021: new developments and updates10.1093/nar/gkaa1025Nucleic Acids Res. 49: D498-D508 202033211880