Strain identifier

BacDive ID: 203

Type strain: Yes

Species: Arcanobacterium hippocoleae

Strain Designation: M401624/00/2, 001127, M 601624/00/2

Strain history: CIP <- 2000, CCUG <- M.D. Collins, Inverness, UK: strain 001127 <- G. Foster, Inverness, UK: strain M 601624/00/2

NCBI tax ID(s): 149017 (species)

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General

@ref: 5988

BacDive-ID: 203

DSM-Number: 15539

keywords: genome sequence, 16S sequence, Bacteria, microaerophile, mesophilic, Gram-positive, rod-shaped, colony-forming, animal pathogen

description: Arcanobacterium hippocoleae M401624/00/2 is a microaerophile, mesophilic, Gram-positive animal pathogen that forms circular colonies and was isolated from horse vaginal discharge, 3 year old Arab cross.

NCBI tax id

  • NCBI tax id: 149017
  • Matching level: species

strain history

@refhistory
5988<- CCUG <- M. D. Collins <- G. Foster
119905CIP <- 2000, CCUG <- M.D. Collins, Inverness, UK: strain 001127 <- G. Foster, Inverness, UK: strain M 601624/00/2

doi: 10.13145/bacdive203.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Actinomycetales
  • family: Actinomycetaceae
  • genus: Arcanobacterium
  • species: Arcanobacterium hippocoleae
  • full scientific name: Arcanobacterium hippocoleae Hoyles et al. 2002

@ref: 5988

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Actinomycetaceae

genus: Arcanobacterium

species: Arcanobacterium hippocoleae

full scientific name: Arcanobacterium hippocoleae Hoyles et al. 2002

strain designation: M401624/00/2, 001127, M 601624/00/2

type strain: yes

Morphology

cell morphology

@refgram staincell shapemotility
22973positiverod-shapedno
119905positiverod-shapedno

colony morphology

@refcolony colorcolony shapemedium used
22973shiny, opaque and greycircularblood agar
119905

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
5988BHI/2 MEDIUM (DSMZ Medium 217)yeshttps://mediadive.dsmz.de/medium/217Name: BHI/2 MEDIUM (DSMZ Medium 217) Composition: Brain heart infusion 37.0 g/l Casein hydrolysate 10.0 g/l Glucose 5.0 g/l Yeast extract 5.0 g/l Distilled water
5988COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
22973blood agaryes
40653MEDIUM 6 - Columbia agar with 10 % horse bloodyesDistilled water make up to (1000.000 ml);Columbia agar (39.000 g);Horseblood (100.000 ml)
119905CIP Medium 6yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=6

culture temp

@refgrowthtypetemperaturerange
5988positivegrowth37mesophilic
40653positivegrowth37mesophilic
56440positivegrowth37mesophilic
119905positivegrowth37-41
119905nogrowth10psychrophilic
119905nogrowth15psychrophilic
119905nogrowth25mesophilic
119905nogrowth30mesophilic
119905nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
5988microaerophile
22973facultative anaerobe
56440anaerobe
56440microaerophile

spore formation

  • @ref: 22973
  • spore formation: no

halophily

@refsaltgrowthtested relationconcentration
119905NaClpositivegrowth0 %
119905NaClnogrowth2 %
119905NaClnogrowth4 %
119905NaClnogrowth6 %
119905NaClnogrowth8 %
119905NaClnogrowth10 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2297323456cyclodextrin-builds acid from
2297318333D-arabitol-builds acid from
2297316634raffinose-builds acid from
2297316988D-ribose-builds acid from
2297365327D-xylose-builds acid from
2297328087glycogen-builds acid from
2297330849L-arabinose-builds acid from
2297329864mannitol-builds acid from
229736731melezitose-builds acid from
2297328053melibiose-builds acid from
22973320055methyl beta-D-glucopyranoside-builds acid from
2297327941pullulan-builds acid from
2297330911sorbitol-builds acid from
2297317992sucrose-builds acid from
2297333954tagatose-builds acid from
2297327082trehalose-builds acid from
229735291gelatin-hydrolysis
2297317632nitrate-reduction
2297317634D-glucose+builds acid from
2297317716lactose+builds acid from
229734853esculin+hydrolysis
22973606565hippurate+hydrolysis
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
6837124265gluconate-builds acid from
6837118403L-arabitol-builds acid from
6837118333D-arabitol-builds acid from
6837118287L-fucose-builds acid from
6837128847D-fucose-builds acid from
6837116443D-tagatose-builds acid from
6837162318D-lyxose-builds acid from
6837132528turanose-builds acid from
6837128066gentiobiose-builds acid from
6837117151xylitol-builds acid from
6837128087glycogen-builds acid from
6837128017starch-builds acid from
6837116634raffinose-builds acid from
683716731melezitose-builds acid from
6837115443inulin-builds acid from
6837127082trehalose-builds acid from
6837117992sucrose-builds acid from
6837128053melibiose-builds acid from
6837117057cellobiose-builds acid from
6837117814salicin-builds acid from
683714853esculin-builds acid from
6837118305arbutin-builds acid from
6837127613amygdalin-builds acid from
68371320061methyl alpha-D-glucopyranoside-builds acid from
6837143943methyl alpha-D-mannoside-builds acid from
6837117924D-sorbitol-builds acid from
6837116899D-mannitol-builds acid from
6837116813galactitol-builds acid from
6837162345L-rhamnose-builds acid from
6837117266L-sorbose-builds acid from
6837116024D-mannose-builds acid from
6837115824D-fructose-builds acid from
6837174863methyl beta-D-xylopyranoside-builds acid from
6837115963ribitol-builds acid from
6837165328L-xylose-builds acid from
6837165327D-xylose-builds acid from
6837116988D-ribose-builds acid from
6837130849L-arabinose-builds acid from
6837117108D-arabinose-builds acid from
6837117113erythritol-builds acid from
6837117754glycerol-builds acid from
1199054853esculin-hydrolysis
119905606565hippurate+hydrolysis
11990517632nitrate-reduction
11990516301nitrite-reduction

antibiotic resistance

  • @ref: 119905
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: yes
  • is resistant: no

metabolite production

@refChebi-IDmetaboliteproduction
2297315688acetoinno
11990535581indoleno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
11990515688acetoin-
11990517234glucose-

enzymes

@refvalueactivityec
22973acid phosphatase-3.1.3.2
22973alpha-fucosidase-3.2.1.51
22973alpha-galactosidase-3.2.1.22
22973alpha-glucosidase+3.2.1.20
22973alpha-mannosidase-3.2.1.24
22973arginine dihydrolase-3.5.3.6
22973beta-galactosidase+3.2.1.23
22973beta-glucosidase-3.2.1.21
22973beta-glucuronidase+3.2.1.31
22973beta-mannosidase-3.2.1.25
22973catalase-1.11.1.6
22973chymotrypsin-3.4.4.5
22973esterase (C 4)-
22973esterase lipase (C 8)-
22973glycyl tryptophan arylamidase-
22973leucine arylamidase+3.4.11.1
22973lipase (C 14)-
22973phosphoamidase+3.9.1.1
22973pyrazinamidase-3.5.1.B15
22973pyroglutamic acid arylamidase-
22973pyrrolidonyl arylamidase-3.4.19.3
22973tripeptide aminopeptidase-3.4.11.4
22973trypsin-3.4.21.4
22973urease-3.5.1.5
22973valine arylamidase-
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase+3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase+3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase-3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase+3.1.3.1
119905oxidase-
119905beta-galactosidase-3.2.1.23
119905alcohol dehydrogenase-1.1.1.1
119905gelatinase-
119905amylase+
119905DNase+
119905caseinase-3.4.21.50
119905catalase-1.11.1.6
119905tween esterase-
119905gamma-glutamyltransferase+2.3.2.2
119905lecithinase-
119905lipase-
119905lysine decarboxylase+4.1.1.18
119905ornithine decarboxylase+4.1.1.17
119905phenylalanine ammonia-lyase-4.3.1.24
119905tryptophan deaminase-
119905urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
119905-+++-+-----+--++-+--

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
119905---------+/-+/------+/-----+/------+/-+/---------------------

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentsampling date
5988horse vaginal discharge, 3 year old Arab crossDumfriesUnited KingdomGBREurope
56440Horse vaginal discharge,3 year old Arab cross h.DumfriesUnited KingdomGBREurope2000-09-01
119905Animal, Horse, vaginal dischargeInvernessUnited KingdomGBREurope

isolation source categories

Cat1Cat2Cat3
#Host#Mammals#Equidae (Horse)
#Host Body-Site#Urogenital tract#Vagina
#Host Body Product#Fluids

taxonmaps

  • @ref: 69479
  • File name: preview.99_176229.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_28;96_8113;97_64761;98_115216;99_176229&stattab=map
  • Last taxonomy: Arcanobacterium hippocoleae subclade
  • 16S sequence: AJ300767
  • Sequence Identity:
  • Total samples: 1127
  • soil counts: 46
  • aquatic counts: 59
  • animal counts: 1012
  • plant counts: 10

Safety information

risk assessment

@refpathogenicity animalbiosafety levelbiosafety level comment
5988yes, in single cases1Risk group (German classification)
1199051Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Arcanobacterium hippocoleae strain DSM 15539 16S ribosomal RNA gene and 16S-23S ribosomal RNA intergenic spacer, partial sequenceEU194568518ena149017
22973Arcanobacterium hippocoleae partial 16S rRNA gene, strain CCUG 44697AJ3007671486nuccore149017

Genome sequences

  • @ref: 66792
  • description: Arcanobacterium hippocoleae DSM 15539
  • accession: 2919380674
  • assembly level: draft
  • database: img
  • NCBI tax ID: 149017

External links

@ref: 5988

culture collection no.: DSM 15539, CCUG 44697, CIP 106850

straininfo link

  • @ref: 69886
  • straininfo: 87824

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny11931175Arcanobacterium hippocoleae sp. nov., from the vagina of a horse.Hoyles L, Falsen E, Foster G, Rogerson F, Collins MDInt J Syst Evol Microbiol10.1099/00207713-52-2-6172002Actinomycetaceae/*classification/genetics/isolation & purification, Animals, Female, Horses/*microbiology, Molecular Sequence Data, Phenotype, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Vaginal Discharge/*microbiology/veterinaryGenetics
Phylogeny30593358Isolation and comparison of Arcanobacterium hippocoleae isolates from the genital tract of 15 mares.Pegne JC, Duquesne F, Laugier C, Lequeux G, Petry SVet Microbiol10.1016/j.vetmic.2018.11.0262018Animals, Arcanobacterium/genetics/*isolation & purification, Female, Genitalia/microbiology, Genotype, Horses/*microbiology, Mass Spectrometry/veterinary, Phenotype, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Species Specificity, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/veterinaryGenetics

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
5988Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 15539)https://www.dsmz.de/collection/catalogue/details/culture/DSM-15539
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
22973Lesley Hoyles,Enevold Falsen,Geoffrey Foster,Forbes Rogerson,Matthew D Collins10.1099/00207713-52-2-617Arcanobacterium hippocoleae sp. nov., from the vagina of a horse.IJSEM 52: 617-619 200211931175
40653Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/19026
56440Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 44697)https://www.ccug.se/strain?id=44697
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69886Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID87824.1StrainInfo: A central database for resolving microbial strain identifiers
119905Curators of the CIPCollection of Institut Pasteur (CIP 106850)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20106850