Strain identifier

BacDive ID: 202

Type strain: Yes

Species: Arcanobacterium pluranimalium

Strain Designation: M430/94/2

Strain history: CIP <- 2000, CCUG <- P.A. Lawson, Reading, UK <- G. Foster, Inverness, UK: strain M430/94/2

NCBI tax ID(s): 108028 (species)

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General

@ref: 5060

BacDive-ID: 202

DSM-Number: 13483

keywords: genome sequence, 16S sequence, Bacteria, facultative anaerobe, mesophilic, Gram-positive, animal pathogen

description: Arcanobacterium pluranimalium M430/94/2 is a facultative anaerobe, mesophilic, Gram-positive animal pathogen that was isolated from spleen of dead harbour porpoise.

NCBI tax id

  • NCBI tax id: 108028
  • Matching level: species

strain history

@refhistory
5060<- CCUG <- P.A. Lawson <- G. Foster; M430/94/2
121872CIP <- 2000, CCUG <- P.A. Lawson, Reading, UK <- G. Foster, Inverness, UK: strain M430/94/2

doi: 10.13145/bacdive202.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Actinomycetales
  • family: Actinomycetaceae
  • genus: Arcanobacterium
  • species: Arcanobacterium pluranimalium
  • full scientific name: Arcanobacterium pluranimalium Lawson et al. 2001

@ref: 5060

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Actinomycetaceae

genus: Arcanobacterium

species: Arcanobacterium pluranimalium

full scientific name: Arcanobacterium pluranimalium Lawson et al. 2001

strain designation: M430/94/2

type strain: yes

Morphology

cell morphology

@refgram stainmotilityconfidencecell shape
22972positiveno
69480no90.089
69480positive100
121872positivenorod-shaped

colony morphology

@reftype of hemolysishemolysis ability
22972alpha1
1218721

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
5060COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
35947MEDIUM 6 - Columbia agar with 10 % horse bloodyesDistilled water make up to (1000.000 ml);Columbia agar (39.000 g);Horseblood (100.000 ml)
121872CIP Medium 6yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=6

culture temp

@refgrowthtypetemperaturerange
5060positivegrowth37mesophilic
35947positivegrowth30mesophilic
55474positivegrowth37mesophilic
121872positivegrowth25-41
121872nogrowth10psychrophilic
121872nogrowth15psychrophilic
121872nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
22972facultative anaerobe
55474microaerophile
121872facultative anaerobe

spore formation

@refspore formationconfidence
69481no100
69480no99.999

halophily

@refsaltgrowthtested relationconcentration
121872NaClpositivegrowth0-2 %
121872NaClnogrowth4 %
121872NaClnogrowth6 %
121872NaClnogrowth8 %
121872NaClnogrowth10 %

murein

@refmurein short keytype
5060A11.51A5alpha L-Lys-L-Lys-D-Glu
22972A11.51A5alpha L-Lys-L-Lys-D-Glu

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2297223456cyclodextrin-builds acid from
2297218333D-arabitol-builds acid from
2297216634raffinose-builds acid from
2297265327D-xylose-builds acid from
2297228087glycogen-builds acid from
2297230849L-arabinose-builds acid from
2297217716lactose-builds acid from
2297229864mannitol-builds acid from
229726731melezitose-builds acid from
2297228053melibiose-builds acid from
2297227941pullulan-builds acid from
2297230911sorbitol-builds acid from
2297217992sucrose-builds acid from
2297233954tagatose-builds acid from
2297227082trehalose-builds acid from
2297217632nitrate-reduction
2297217634D-glucose+builds acid from
2297216988D-ribose+builds acid from
229724853esculin+hydrolysis
229725291gelatin+hydrolysis
22972606565hippurate+hydrolysis
2297217306maltose+/-builds acid from
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
6837124265gluconate-builds acid from
6837118403L-arabitol-builds acid from
6837118333D-arabitol-builds acid from
6837118287L-fucose-builds acid from
6837128847D-fucose-builds acid from
6837116443D-tagatose-builds acid from
6837162318D-lyxose-builds acid from
6837132528turanose-builds acid from
6837128066gentiobiose-builds acid from
6837117151xylitol-builds acid from
6837128087glycogen-builds acid from
6837128017starch-builds acid from
6837116634raffinose-builds acid from
683716731melezitose-builds acid from
6837115443inulin-builds acid from
6837127082trehalose-builds acid from
6837117992sucrose-builds acid from
6837128053melibiose-builds acid from
6837117716lactose-builds acid from
6837117306maltose+builds acid from
6837117057cellobiose-builds acid from
6837117814salicin-builds acid from
683714853esculin-builds acid from
6837118305arbutin-builds acid from
6837127613amygdalin-builds acid from
6837159640N-acetylglucosamine+builds acid from
68371320061methyl alpha-D-glucopyranoside-builds acid from
6837143943methyl alpha-D-mannoside-builds acid from
6837117924D-sorbitol-builds acid from
6837116899D-mannitol-builds acid from
6837116813galactitol-builds acid from
6837162345L-rhamnose-builds acid from
6837117266L-sorbose-builds acid from
6837116024D-mannose+builds acid from
6837115824D-fructose+builds acid from
6837117634D-glucose+builds acid from
6837112936D-galactose+builds acid from
6837174863methyl beta-D-xylopyranoside-builds acid from
6837115963ribitol-builds acid from
6837165328L-xylose-builds acid from
6837165327D-xylose-builds acid from
6837116988D-ribose+builds acid from
6837130849L-arabinose-builds acid from
6837117108D-arabinose-builds acid from
6837117113erythritol-builds acid from
6837117754glycerol-builds acid from
1218724853esculin-hydrolysis
121872606565hippurate-hydrolysis
12187217632nitrate-reduction
12187216301nitrite-reduction
12187217632nitrate+respiration
6838129016arginine+hydrolysis
6838116988D-ribose+builds acid from
6838116899D-mannitol-builds acid from
6838130911sorbitol-builds acid from
6838117716lactose-builds acid from
6838127082trehalose-builds acid from
6838116634raffinose-builds acid from
6838117992sucrose-builds acid from
6838130849L-arabinose-builds acid from
6838118333D-arabitol-builds acid from
6838140585alpha-cyclodextrin-builds acid from
68381606565hippurate+hydrolysis
6838128087glycogen-builds acid from
6838127941pullulan-builds acid from
6838117306maltose-builds acid from
6838128053melibiose-builds acid from
683816731melezitose-builds acid from
68381320055methyl beta-D-glucopyranoside-builds acid from
6838116443D-tagatose-builds acid from
6838116199urea-hydrolysis

metabolite production

@refChebi-IDmetaboliteproduction
2297215688acetoinno
12187235581indoleno
6838115688acetoinno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
6838115688acetoin-
12187215688acetoin-
12187217234glucose+

enzymes

@refvalueactivityec
22972alkaline phosphatase-3.1.3.1
22972alpha-galactosidase-3.2.1.22
22972alpha-glucosidase-3.2.1.20
22972arginine dihydrolase-3.5.3.6
22972beta-galactosidase-3.2.1.23
22972beta-glucosidase-3.2.1.21
22972beta-glucuronidase+3.2.1.31
22972beta-glucuronidase-3.2.1.31
22972beta-mannosidase-3.2.1.25
22972catalase+1.11.1.6
22972N-acetyl-beta-glucosaminidase-3.2.1.52
22972pyroglutamic acid arylamidase+
22972tripeptide aminopeptidase+3.4.11.4
22972urease-3.5.1.5
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase+3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase+3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin+3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase+3.1.3.1
68381urease-3.5.1.5
68381beta-mannosidase-3.2.1.25
68381glycyl tryptophan arylamidase-
68381N-acetyl-beta-glucosaminidase-3.2.1.52
68381pyrrolidonyl arylamidase+3.4.19.3
68381beta-galactosidase-3.2.1.23
68381Alanyl-Phenylalanyl-Proline arylamidase+
68381alkaline phosphatase-3.1.3.1
68381alpha-galactosidase-3.2.1.22
68381beta-glucuronidase+3.2.1.31
68381beta-glucosidase-3.2.1.21
68381arginine dihydrolase+3.5.3.6
121872oxidase-
121872beta-galactosidase+3.2.1.23
121872alcohol dehydrogenase-1.1.1.1
121872gelatinase-
121872amylase-
121872DNase+
121872caseinase+3.4.21.50
121872catalase+1.11.1.6
121872tween esterase-
121872gamma-glutamyltransferase+2.3.2.2
121872lecithinase+
121872lipase+
121872lysine decarboxylase-4.1.1.18
121872ornithine decarboxylase-4.1.1.17
121872phenylalanine ammonia-lyase+4.3.1.24
121872protease+
121872tryptophan deaminase-
121872urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
121872-+++-+--+-++---+--+-

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
121872----+----++++---+/-----+-----+---------------------

API rID32STR

@refADH Argbeta GLUbeta GARbeta GURalpha GALPALRIBMANSORLACTRERAFSACLARADARLCDEXVPAPPAbeta GALPyrAbeta NAGGTAHIPGLYGPULMALMELMLZMbeta DGTAGbeta MANURE
5060+--+--+----------+-+--+---------

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinentsampling dategeographic location
5060spleen of dead harbour porpoiseUnited KingdomGBREurope
55474Dead harbour porpoiseUnited KingdomGBREurope1994Inverness
121872Dead harbour porpoiseUnited KingdomGBREuropeInverness

isolation source categories

Cat1Cat2Cat3
#Host#Other#Decomposing animal
#Host#Mammals#Aquatic mammal
#Host Body-Site#Organ#Spleen

taxonmaps

  • @ref: 69479
  • File name: preview.99_98953.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_28;96_37426;97_47584;98_62378;99_98953&stattab=map
  • Last taxonomy: Arcanobacterium
  • 16S sequence: AJ250959
  • Sequence Identity:
  • Total samples: 2212
  • soil counts: 51
  • aquatic counts: 67
  • animal counts: 2070
  • plant counts: 24

Safety information

risk assessment

@refpathogenicity animalbiosafety levelbiosafety level comment
5060yes2Risk group (German classification)
1218721Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Arcanobacterium pluranimalium strain DSM 13483 16S ribosomal RNA gene and 16S-23S ribosomal RNA intergenic spacer, partial sequenceEU194567492ena108028
5060Arcanobacterium sp. partial 16S rRNA gene, strain M430/94/2AJ2509591476ena108028

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Arcanobacterium pluranimalium DSM 13483GCA_016907845contigncbi108028
66792Arcanobacterium pluranimalium strain DSM 13483108028.3wgspatric108028
66792Arcanobacterium pluranimalium DSM 134832893686931draftimg108028

GC content

  • @ref: 5060
  • GC-content: 57

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
motileno95.28yes
flagellatedno97.172no
gram-positiveyes92.283yes
anaerobicno92.335yes
aerobicno82.062no
halophileno91.378no
spore-formingno96.501no
thermophileno93.776yes
glucose-utilyes87.445no
glucose-fermentyes72.579no

External links

@ref: 5060

culture collection no.: DSM 13483, CCUG 42575, CIP 106442

straininfo link

  • @ref: 69885
  • straininfo: 14612

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny11211273Arcanobacterium pluranimalium sp. nov., isolated from porpoise and deer.Lawson PA, Falsen E, Foster G, Eriksson E, Weiss N, Collins MDInt J Syst Evol Microbiol10.1099/00207713-51-1-552001Actinomycetaceae/*classification/genetics/isolation & purification, Actinomycetales Infections/microbiology/*veterinary, Animals, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Deer/*microbiology, Genes, rRNA, Molecular Sequence Data, Phenotype, Phylogeny, Porpoises/*microbiology, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNAGenetics
Phylogeny26464930Phenotypical and Genotypical Properties of an Arcanobacterium pluranimalium Strain Isolated from a Juvenile Giraffe (Giraffa camelopardalis reticulata).Risse K, Schlez K, Eisenberg T, Geiger C, Balbutskaya A, Sammra O, Lammler C, Abdulmawjood AJ Vet Med10.1155/2014/4087242014
Phylogeny32589570Arcanobacterium bovis sp. nov., isolated from the milk of a cow with mastitis.Sammra O, Foster G, Hassan AA, Alssahen M, Lammler C, Glaeser SP, Kampfer P, Busse HJ, Borowiak M, Malorny B, Ritchie CM, Prenger-Berninghoff E, Abdulmawjood AInt J Syst Evol Microbiol10.1099/ijsem.0.0042302020Animals, Arcanobacterium/*classification/isolation & purification, Bacterial Typing Techniques, Base Composition, Cattle, DNA, Bacterial/genetics, Fatty Acids/chemistry, Female, Glycolipids/chemistry, Mastitis, Bovine/*microbiology, Milk/*microbiology, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Vitamin K 2/analogs & derivatives/chemistry, Whole Genome SequencingTranscriptome
Genetics33854785Taxonogenomics description of Arcanobacterium urinimassiliense sp. nov., a new bacterial species isolated from urine sample.Ben Khedher M, Lo CI, Diop K, Morand A, Armstrong N, Raoult D, Fenollar FNew Microbes New Infect10.1016/j.nmni.2021.1008542021Phylogeny

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
5060Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 13483)https://www.dsmz.de/collection/catalogue/details/culture/DSM-13483
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
22972P. A. Lawson,E. Falsen,G. Foster,E. Eriksson,N. Weiss,M. D. Collins10.1099/00207713-51-1-55Arcanobacterium pluranimalium sp. nov., isolated from porpoise and deer.IJSEM 51: 55-59 200111211273
35947Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/18573
55474Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 42575)https://www.ccug.se/strain?id=42575
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68371Automatically annotated from API 50CH acid
68381Automatically annotated from API rID32STR
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
69885Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID14612.1StrainInfo: A central database for resolving microbial strain identifiers
121872Curators of the CIPCollection of Institut Pasteur (CIP 106442)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20106442