Strain identifier
BacDive ID: 20038
Type strain: 
Species: Sorangium cellulosum
Strain Designation: So ce702
NCBI tax ID(s): 56 (species)
version 8.1 (current version)
General
@ref: 18281
BacDive-ID: 20038
keywords: Bacteria
description: Sorangium cellulosum So ce702 is a bacterium that builds a fruiting body and was isolated from soil with plant residues from grain field.
NCBI tax id
- NCBI tax id: 56
- Matching level: species
doi: 10.13145/bacdive20038.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Deltaproteobacteria
- order: Myxococcales
- family: Polyangiaceae
- genus: Sorangium
- species: Sorangium cellulosum
- full scientific name: Sorangium cellulosum (Brockman 1989 ex Imshenetski and Solntseva 1936) Reichenbach 2007
synonyms
- @ref: 20215
- synonym: Polyangium cellulosum
@ref: 18281
domain: Bacteria
phylum: Proteobacteria
class: Deltaproteobacteria
order: Myxococcales
family: Sandaracinaceae
genus: Sorangium
species: Sorangium cellulosum
full scientific name: Sorangium cellulosum
strain designation: So ce702
type strain: no
Morphology
multicellular morphology
- @ref: 18281
- forms multicellular complex: yes
- complex name: fruiting body
Culture and growth conditions
culture medium
| @ref | name | growth | composition |
|---|---|---|---|
| 18281 | CA13 AGAR | yes | Ingredients: Stock solution 1 (in 100ml): (NH4)2SO4 0.5g K2HPO4 7.5g Stock solution 2 (in 100ml): MgSO4 x 7H2O 1.5g Stock solution 3 (in 100ml): CaCl2 0.2g FeCl3 0.15g Basal medium: Agar 1.5% Preparation / Remarks: after autoclaving to: a) 1% (v/v) of the 3 stock solutions b) 1% glucose c) trace elements |
| 18281 | CA2 AGAR | yes | Ingredients: Stock solution 1 (in 100ml): KNO3 7.5g K2HPO4 7.5g Stock solution 2 (in 100ml): MgSO4 x 7H2O 1.5g Stock solution 3 (in 100ml): CaCl2 0.2g FeCl3 0.15g Basal medium: Agar 1.5% Preparation / Remarks: after autoclaving to: a) 1% (v/v) of the 3 stock solutions b) 1% glucose c) trace elements |
| 18281 | CHITIN 7 AGAR | yes | Covering agar (thin layer): MgSO4 x 7H2O 0.1% K2HPO4 0.02% Agar 1.5% Preparation / Remarks: pH 7.2 After autoclaving add 30 mL of sterile Chitin suspension (22.5 g/L Chitin in H2O) to 100 mL of the medium and pour on w-agar plates |
| 18281 | CY-AGAR | yes | Ingredients: Casitone(Difco) 0.3% yeast extract 0.1% CaCl2 0.1% Agar 1.5% Preparation / Remarks: pH 7.2 (KOH) autoclaved |
| 18281 | KAN 5 AGAR | yes | Ingredients: Baker's yeast 0.5% (based on fresh weight) CaCl2 0.1% Agar 1.5% Vitamine B12 0.5 µg/ml Preparation / Remarks: pH 7.2 autoclaved Combine with 250 mg/L Kanamycin-sulfate (sterilized by filtration) |
| 18281 | Myx MEDIUM | yes | Ingredients: Peptone (Difco) 1% Glucose 1% Yeast extract (Difco) 0.1% Phosphate buffer 0.01 M pH 6.5 Aerobacter aerogenes (sterilized with formalin) 500 mg/L (dry weight) as described by Kerr, N.S. (1963) The Growth of Myxamoebae of the True Slime Mould, Didymium nigripes, in Axenic Culture. J Gen Microbiol 32: 409-416. |
| 18281 | SKIM MILK 1 AGAR | yes | Ingredients: cy-Agar 300 ml Skim milk (Oxoid) 15g H2O 60 ml Preparation / Remarks: 300 ml cy-agar autoclaved. Separately 15 g skim milk (Oxoid) in 60 ml water and exactly 15 min autoclaving at 115 °C. Mix the two and pour a thin layer on cy-plates. |
| 18281 | STAN 21 AGAR with Filter | yes | Ingredients: 1. Agar 1% K2HPO4 0.1% Yeast extract (Difco) 0.002% 2. (NH4)2SO4 0.1% MgSO4 x 7H2O 0.1% CaCl2 x 2H2O 0.1% MnSO4 x H2O 0.01% FeCl3 x 6H2O 0.02% MnSO4 x 7H2O 0.01% FeCl3 0.02% Traceelement-solution 1ml to 100 ml Preparation / Remarks: 1. in 2/3 of the volume after autoclaving 1 + 2 together and pour plates. Hang up a sterile-filter paper |
| 18281 | TOLI AGAR Kan4 | yes | Ingredients: Agar (Difco) 1.5% CaCl2 0.1% Cycloheximide 2.5 mg per 100 ml Preparation / Remarks: pH 7.2 (KOH) autoclaved w-agar with 2.5 mg of cycloheximide per 100 ml, the antibiotic is filter sterilized and added to the agar just before pouring Cycloheximide does not seem to affect growth of myxobakteria, whereas fungi and amoebae are often inhibited. Combine with 250 mg/L Kanamycin-sulfate (sterilized by filtration) Pour the plates and streak autoclaved E.coli cells |
| 18281 | VY/2 AGAR | yes | Ingredients: Baker's yeast 0.5% (based on fresh weight) CaCl2 0.1% Agar 1.5% Vitamin B12 0.05 % Preparation / Remarks: pH 7.2 autoclaved |
Physiology and metabolism
observation
| @ref | observation |
|---|---|
| 18281 | Ability for casein degradation |
| 18281 | No ability for chitin degradation |
antibiotic resistance
| @ref | ChEBI | group ID | metabolite | is antibiotic | is resistant | resistance conc. |
|---|---|---|---|---|---|---|
| 18281 | 6104 | 3 | kanamycin | yes | yes | 250 µg/mL |
| 18281 | 27641 | 3 | cycloheximide | yes | yes | 25 µg/mL |
| 18281 | 6104 | kanamycin | yes | yes | 250 µg/mL |
Isolation, sampling and environmental information
isolation
- @ref: 18281
- sample type: soil with plant residues from grain field
- sampling date: 1989-08-01
- geographic location: near Wolfenbuettel
- country: Germany
- origin.country: DEU
- continent: Europe
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Engineered | #Agriculture | #Field |
| #Environmental | #Terrestrial | #Soil |
| #Host | #Plants | #Herbaceous plants (Grass,Crops) |
External links
@ref: 18281
Reference
| @id | authors | title | doi/url |
|---|---|---|---|
| 18281 | Reichenbach, H. | Collection description and documentation of myxobacteria by H. Reichenbach, HZI (formerly GBF); Collection curr. located at the DSMZ | |
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | 10.1099/ijsem.0.004332 |