Strain identifier

BacDive ID: 1984

Type strain: Yes

Species: Burkholderia diffusa

Strain Designation: AU1075, O85

Strain history: <- CCUG <- E. Vanlaere, LMG <- J. J. LiPuma

NCBI tax ID(s): 488732 (species)

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General

@ref: 17206

BacDive-ID: 1984

DSM-Number: 23434

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, Gram-negative, rod-shaped

description: Burkholderia diffusa AU1075 is an aerobe, mesophilic, Gram-negative bacterium that was isolated from sputum of patient with cystic fibrosis.

NCBI tax id

  • NCBI tax id: 488732
  • Matching level: species

strain history

  • @ref: 17206
  • history: <- CCUG <- E. Vanlaere, LMG <- J. J. LiPuma

doi: 10.13145/bacdive1984.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Betaproteobacteria
  • order: Burkholderiales
  • family: Burkholderiaceae
  • genus: Burkholderia
  • species: Burkholderia diffusa
  • full scientific name: Burkholderia diffusa Vanlaere et al. 2008

@ref: 17206

domain: Bacteria

phylum: Proteobacteria

class: Betaproteobacteria

order: Burkholderiales

family: Burkholderiaceae

genus: Burkholderia

species: Burkholderia diffusa

full scientific name: Burkholderia diffusa Vanlaere et al. 2008

strain designation: AU1075, O85

type strain: yes

Morphology

cell morphology

  • @ref: 23365
  • gram stain: negative
  • cell shape: rod-shaped

colony morphology

  • @ref: 17206
  • type of hemolysis: gamma

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
17206COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
17206TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)yeshttps://mediadive.dsmz.de/medium/535Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water

culture temp

@refgrowthtypetemperaturerange
17206positivegrowth30mesophilic
23365positivegrowth30-37mesophilic
23365positivemaximum42thermophilic
60232positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
23365aerobe
60232aerobe

spore formation

  • @ref: 23365
  • spore formation: no

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2336517306maltose-assimilation
2336518222xylose-builds acid from
233654853esculin-hydrolysis
2336517128adipate+assimilation
2336527689decanoate+assimilation
2336516947citrate+assimilation
233658391D-gluconate+assimilation
2336516899D-mannitol+assimilation
2336516024D-mannose+assimilation
2336517234glucose+assimilation
2336530849L-arabinose+assimilation
2336515589L-malate+assimilation
23365506227N-acetylglucosamine+assimilation
2336518401phenylacetate+assimilation
2336515963ribitol+builds acid from
2336517716lactose+builds acid from
2336517306maltose+builds acid from
2336517992sucrose+builds acid from
2336517632nitrate+reduction
6836925115malate+assimilation
6836917128adipate+assimilation
6836927689decanoate+assimilation
6836924265gluconate+assimilation
6836917306maltose-assimilation
6836959640N-acetylglucosamine+assimilation
6836916899D-mannitol+assimilation
6836916024D-mannose+assimilation
6836930849L-arabinose+assimilation
6836917634D-glucose+assimilation
683695291gelatin-hydrolysis
683694853esculin-hydrolysis
6836916199urea-hydrolysis
6836929016arginine-hydrolysis
6836917634D-glucose+fermentation
6836927897tryptophan-energy source
6836917632nitrate+reduction

metabolite production

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
17206catalase+1.11.1.6
17206cytochrome-c oxidase+1.9.3.1
23365arginine dihydrolase-3.5.3.6
23365beta-galactosidase+3.2.1.23
23365gelatinase+
23365lysine decarboxylase+4.1.1.18
23365ornithine decarboxylase-4.1.1.17
68369cytochrome oxidase+1.9.3.1
68369gelatinase-
68369beta-glucosidase-3.2.1.21
68369urease-3.5.1.5
68369arginine dihydrolase-3.5.3.6

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPACOX
17206+-+----++++++-+++++++

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinentsampling date
17206sputum of patient with cystic fibrosisUSAUSANorth America
60232Human sputum,cystic fibrosisUSAUSANorth America2001-07-24

isolation source categories

Cat1Cat2Cat3
#Infection#Disease#Cystic fibrosis
#Infection#Patient
#Host Body Product#Fluids#Sputum

taxonmaps

  • @ref: 69479
  • File name: preview.99_198.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_12;96_18;97_18;98_21;99_198&stattab=map
  • Last taxonomy: Burkholderia
  • 16S sequence: AM747629
  • Sequence Identity:
  • Total samples: 5054
  • soil counts: 1055
  • aquatic counts: 564
  • animal counts: 1930
  • plant counts: 1505

Safety information

risk assessment

  • @ref: 17206
  • biosafety level: 2
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 17206
  • description: Burkholderia diffusa partial 16S rRNA gene, type strain R-15930T
  • accession: AM747629
  • length: 1498
  • database: ena
  • NCBI tax ID: 488732

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Burkholderia diffusa DSM 23434GCA_016836765contigncbi488732
66792Burkholderia diffusa LMG 24065GCA_902499115contigncbi488732
66792Burkholderia diffusa CCUG 54558GCA_008802145contigncbi488732
66792Burkholderia diffusa strain CCUG 54558488732.25wgspatric488732
66792Burkholderia diffusa strain DSM 23434488732.34wgspatric488732
66792Burkholderia diffusa strain LMG 24065488732.29wgspatric488732

GC content

  • @ref: 23365
  • GC-content: 67.0

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
gram-positiveno98.602no
anaerobicno98.292yes
halophileno93.941no
spore-formingno95.557yes
glucose-utilyes92.576no
aerobicyes90.416no
thermophileno99.486no
motileyes89.213no
flagellatedno81.902no
glucose-fermentno85.215yes

External links

@ref: 17206

culture collection no.: DSM 23434, CCUG 54558, LMG 24065

straininfo link

  • @ref: 71631
  • straininfo: 358911

literature

  • topic: Phylogeny
  • Pubmed-ID: 18599699
  • title: Burkholderia latens sp. nov., Burkholderia diffusa sp. nov., Burkholderia arboris sp. nov., Burkholderia seminalis sp. nov. and Burkholderia metallica sp. nov., novel species within the Burkholderia cepacia complex.
  • authors: Vanlaere E, Lipuma JJ, Baldwin A, Henry D, De Brandt E, Mahenthiralingam E, Speert D, Dowson C, Vandamme P
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.65634-0
  • year: 2008
  • mesh: Base Sequence, Burkholderia/chemistry/*classification/genetics, Burkholderia cepacia complex/*classification, Genes, Bacterial/genetics, Molecular Sequence Data, Nucleic Acid Hybridization, Phylogeny, Polymorphism, Restriction Fragment Length, RNA, Ribosomal, 16S/genetics, Rec A Recombinases/genetics, Species Specificity
  • topic2: Genetics

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
17206Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 23434)https://www.dsmz.de/collection/catalogue/details/culture/DSM-23434
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
23365Elke Vanlaere, John J. LiPuma, Adam Baldwin, Deborah Henry, Evie De Brandt, Eshwar Mahenthiralingam, David Speert, Chris Dowson, Peter Vandamme10.1099/ijs.0.65634-0Burkholderia latens sp. nov., Burkholderia diffusa sp. nov., Burkholderia arboris sp. nov., Burkholderia seminalis sp. nov. and Burkholderia metallica sp. nov., novel species within the Burkholderia cepacia complexIJSEM 58: 1580-1590 200818599699
60232Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 54558)https://www.ccug.se/strain?id=54558
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68369Automatically annotated from API 20NE
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
71631Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID358911.1StrainInfo: A central database for resolving microbial strain identifiers