Strain identifier

BacDive ID: 1886

Type strain: Yes

Species: Brucella gallinifaecis

Strain Designation: Iso-196

Strain history: CIP <- 2002, P. Kämpfer, Giessen Univ., Giessen, Germany: strain Iso-196

NCBI tax ID(s): 215590 (species)

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General

@ref: 5833

BacDive-ID: 1886

DSM-Number: 15295

keywords: 16S sequence, Bacteria, aerobe, mesophilic, Gram-negative, rod-shaped

description: Brucella gallinifaecis Iso-196 is an aerobe, mesophilic, Gram-negative bacterium that was isolated from faeces of chicken.

NCBI tax id

  • NCBI tax id: 215590
  • Matching level: species

strain history

@refhistory
5833<- P. Kämpfer; Iso196 <- T. Andreae
119518CIP <- 2002, P. Kämpfer, Giessen Univ., Giessen, Germany: strain Iso-196

doi: 10.13145/bacdive1886.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Alphaproteobacteria
  • order: Hyphomicrobiales
  • family: Brucellaceae
  • genus: Brucella
  • species: Brucella gallinifaecis
  • full scientific name: Brucella gallinifaecis (Kämpfer et al. 2003) Hördt et al. 2020

  • synonyms

    • @ref: 20215
    • synonym: Ochrobactrum gallinifaecis

@ref: 5833

domain: Bacteria

phylum: Proteobacteria

class: Alphaproteobacteria

order: Rhizobiales

family: Brucellaceae

genus: Brucella

species: Brucella gallinifaecis

full scientific name: Brucella gallinifaecis (Kämpfer et al. 2003) Hördt et al. 2020

strain designation: Iso-196

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell shapemotility
23336negative2 µmrod-shapedno
119518negativeoval-shapedno

colony morphology

@refcolony sizecolony colorincubation periodhemolysis ability
233362 mmbeige, translucent1 day
1195181

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
5833NUTRIENT AGAR (DSMZ Medium 1)yeshttps://mediadive.dsmz.de/medium/1Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water
23336MacConkey agaryes
23336Nutrient agar (NA)yes
23336Reasoner's 2A agar (R2A)yes
23336Trypticase Soy Agar (TSA)yes
38228MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
119518CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
5833positivegrowth25mesophilic
23336positivegrowth25-30mesophilic
38228positivegrowth25mesophilic
58069positivegrowth30mesophilic
58069positivegrowth10-37
119518positivegrowth15-37
119518nogrowth5psychrophilic
119518nogrowth41thermophilic
119518nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
58069aerobe
119518obligate aerobe

spore formation

  • @ref: 23336
  • spore formation: no

halophily

@refsaltgrowthtested relationconcentration
119518NaClpositivegrowth0-6 %
119518NaClnogrowth8 %
119518NaClnogrowth10 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
23336370543-hydroxybutyrate-assimilation
2333616383cis-aconitate-assimilation
2333616947citrate-assimilation
2333615824D-fructose-assimilation
2333617924D-sorbitol-assimilation
2333617268myo-inositol-assimilation
2333662345L-rhamnose-assimilation
2333691050p-nitrophenyl phenyl phosphonate-hydrolysis
23336620642,3-butanediol-respiration
23336181014-hydroxyphenylacetic acid-respiration
2333618024D-galacturonic acid-respiration
2333628053melibiose-respiration
2333616634raffinose-respiration
2333623652dextrin-respiration
2333628066gentiobiose-respiration
2333629042glucose 1-phosphate-respiration
2333618183L-pyroglutamic acid-respiration
23336320055methyl beta-D-glucopyranoside-respiration
2333617148putrescine-respiration
2333617748thymidine-respiration
2333617151xylitol-respiration
23336178794-hydroxybenzoate-
2333615963ribitol-
2333617057cellobiose-
2333617306maltose-
2333616551D-trehalose-
2333615603L-leucine-
2333668428maltitol-
2333628037N-acetylgalactosamine-
23336506227N-acetylglucosamine-
233369300suberic acid-
2333617992sucrose-
2333615708trans-aconitate-
23336L-proline-4-nitroanilide+hydrolysis
23336645522-hydroxybutyrate+respiration
23336309162-oxoglutarate+respiration
23336370543-hydroxybutyrate+respiration
23336167244-hydroxybutyrate+respiration
2333630089acetate+respiration
2333640585alpha-cyclodextrin+respiration
2333617925alpha-D-glucose+respiration
2333615570D-alanine+respiration
2333618333D-arabitol+respiration
2333615824D-fructose+respiration
2333612936D-galactose+respiration
233368391D-gluconate+respiration
2333616899D-mannitol+respiration
2333616024D-mannose+respiration
2333627605D-psicose+respiration
2333617924D-sorbitol+respiration
2333628087glycogen+respiration
2333617596inosine+respiration
2333621217L-alaninamide+respiration
2333616977L-alanine+respiration
2333673786L-alanylglycine+respiration
2333630849L-arabinose+respiration
2333617196L-asparagine+respiration
2333618287L-fucose+respiration
2333629985L-glutamate+respiration
2333662345L-rhamnose+respiration
2333617306maltose+respiration
2333651850methyl pyruvate+respiration
2333617268myo-inositol+respiration
23336506227N-acetylglucosamine+respiration
2333673784glycyl-l-glutamate+respiration
2333617272propionate+respiration
2333630031succinate+respiration
2333617992sucrose+respiration
2333632528turanose+respiration
2333653423tween 40+respiration
2333653426tween 80+respiration
2333616704uridine+respiration
2333629991L-aspartate+
2333615971L-histidine+
2333616865gamma-aminobutyric acid+/-
2333616958beta-alanine+/-
2333661993maltotrioserespiration
2333617164stachyoserespiration
6836925115malate+assimilation
6836917128adipate-assimilation
6836927689decanoate-assimilation
6836924265gluconate+assimilation
6836917306maltose-assimilation
6836959640N-acetylglucosamine-assimilation
6836916899D-mannitol-assimilation
6836930849L-arabinose+assimilation
6836917634D-glucose+assimilation
683695291gelatin-hydrolysis
683694853esculin-hydrolysis
6836916199urea+hydrolysis
6836917634D-glucose-fermentation
6836927897tryptophan-energy source
6836917632nitrate-reduction
119518606565hippurate-hydrolysis
11951817632nitrate+reduction
11951816301nitrite-reduction
11951817632nitrate-respiration

antibiotic resistance

  • @ref: 119518
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

@refChebi-IDmetaboliteproduction
6836935581indoleno
11951835581indoleno

metabolite tests

@refChebi-IDmetaboliteindole testvoges-proskauer-testmethylred-test
6836935581indole-
11951815688acetoin-
11951817234glucose-

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase-
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin+3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)-
68382esterase (C 4)+
68382alkaline phosphatase+3.1.3.1
68369cytochrome oxidase+1.9.3.1
68369gelatinase-
68369beta-glucosidase-3.2.1.21
68369urease+3.5.1.5
119518oxidase+
119518beta-galactosidase+3.2.1.23
119518alcohol dehydrogenase-1.1.1.1
119518gelatinase-
119518amylase-
119518DNase-
119518caseinase-3.4.21.50
119518catalase+1.11.1.6
119518tween esterase-
119518gamma-glutamyltransferase+2.3.2.2
119518lecithinase-
119518lipase-
119518lysine decarboxylase-4.1.1.18
119518ornithine decarboxylase-4.1.1.17
119518phenylalanine ammonia-lyase-4.3.1.24
119518protease-
119518tryptophan deaminase-
119518urease+3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
119518-++--+--+-+---------

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPACOX
5833----+---+++/----+--+-+/-+
5833----+---+++/----+--+--+
5833---++---+++---+--+--+

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
119518+------------------+-------------------------------------------------------------------------------

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentisolation date
5833faeces of chickenMarburgGermanyDEUEurope
58069Chicken fecesMarburgGermanyDEUEurope
119518Chicken, faecesGermanyDEUEurope2001

isolation source categories

Cat1Cat2Cat3
#Host#Birds#Chicken
#Host Body Product#Gastrointestinal tract#Feces (Stool)

taxonmaps

  • @ref: 69479
  • File name: preview.99_150213.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_5;96_4733;97_73787;98_99281;99_150213&stattab=map
  • Last taxonomy: Ochrobactrum gallinifaecis subclade
  • 16S sequence: AJ519939
  • Sequence Identity:
  • Total samples: 17
  • soil counts: 7
  • aquatic counts: 2
  • animal counts: 8

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
58331Risk group (German classification)
1195181Risk group (French classification)

Sequence information

16S sequences

  • @ref: 23336
  • description: Ochrobactrum gallinifaecis 16S rRNA gene
  • accession: AJ519939
  • length: 1453
  • database: nuccore
  • NCBI tax ID: 215590

External links

@ref: 5833

culture collection no.: DSM 15295, CCUG 48291, CIP 107753, ISO Iso196, ISO 196

straininfo link

  • @ref: 71530
  • straininfo: 100831

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny12807218Towards a standardized format for the description of a novel species (of an established genus): Ochrobactrum gallinifaecis sp. nov.Kampfer P, Buczolits S, Albrecht A, Busse HJ, Stackebrandt EInt J Syst Evol Microbiol10.1099/ijs.0.02710-02003Bacterial Typing Techniques/*standards, Brucella/*classification/genetics/metabolism/physiology, Classification/*methods, DNA, Ribosomal/analysis, Genes, rRNA, Genotype, Molecular Sequence Data, Nucleic Acid Hybridization, Phenotype, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNAGenetics
Phylogeny16825649Ochrobactrum oryzae sp. nov., an endophytic bacterial species isolated from deep-water rice in India.Tripathi AK, Verma SC, Chowdhury SP, Lebuhn M, Gattinger A, Schloter MInt J Syst Evol Microbiol10.1099/ijs.0.63934-02006Carbohydrate Metabolism, Carboxylic Acids/metabolism, Cell Wall/chemistry, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Fatty Acids/analysis/chemistry, Genes, rRNA, India, Molecular Sequence Data, Nucleic Acid Hybridization, Ochrobactrum/*classification/genetics/*isolation & purification/physiology, Oryza/*microbiology, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sequence Homology, Nucleic Acid, Water MicrobiologyMetabolism

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
5833Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 15295)https://www.dsmz.de/collection/catalogue/details/culture/DSM-15295
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
23336Peter Kämpfer, Sandra Buczolits, Andreas Albrecht, Hans-Jürgen Busse, Erko Stackebrandt10.1099/ijs.0.02710-0Towards a standardized format for the description of a novel species (of an established genus): Ochrobactrum gallinifaecis sp. nov.IJSEM 53: 893-896 200312807218
38228Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/5223
58069Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 48291)https://www.ccug.se/strain?id=48291
68369Automatically annotated from API 20NE
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
71530Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID100831.1StrainInfo: A central database for resolving microbial strain identifiers
119518Curators of the CIPCollection of Institut Pasteur (CIP 107753)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20107753