Strain identifier

BacDive ID: 1868

Type strain: Yes

Species: Brevibacterium album

Strain history: <- S-K Tang, Yunnan Inst. Microbiol, China

NCBI tax ID(s): 1121122 (strain), 417948 (species)

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General

@ref: 7463

BacDive-ID: 1868

DSM-Number: 18261

keywords: genome sequence, 16S sequence, Bacteria, aerobe, Gram-positive, motile, rod-shaped

description: Brevibacterium album DSM 18261 is an aerobe, Gram-positive, motile bacterium that was isolated from saline alkali soil.

NCBI tax id

NCBI tax idMatching level
417948species
1121122strain

strain history

@refhistory
7463<- Shu-Kun Tang; YIM 90718
67770KCTC 19173 <-- W.-J. Li YIM 90718.
67771<- S-K Tang, Yunnan Inst. Microbiol, China

doi: 10.13145/bacdive1868.20240916.9.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Micrococcales
  • family: Brevibacteriaceae
  • genus: Brevibacterium
  • species: Brevibacterium album
  • full scientific name: Brevibacterium album Tang et al. 2008

@ref: 7463

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Brevibacteriaceae

genus: Brevibacterium

species: Brevibacterium album

full scientific name: Brevibacterium album Tang et al. 2008

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotilityconfidence
32244positive4.5 µm1.8 µmrod-shapedyes
67771positive
69480no90.5

colony morphology

@refcolony colorincubation periodmedium used
19762Ivory (1014)10-14 daysISP 2
19762Ivory (1014)10-14 daysISP 3
19762Ivory (1014)10-14 daysISP 4
19762Ivory (1014)10-14 daysISP 5
19762Ivory (1014)10-14 daysISP 6
19762Ivory (1014)10-14 daysISP 7

multicellular morphology

@refforms multicellular complexmedium name
19762noISP 2
19762noISP 3
19762noISP 4
19762noISP 5
19762noISP 6
19762noISP 7

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
7463GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)yeshttps://mediadive.dsmz.de/medium/65Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
19762ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
19762ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
19762ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
19762ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
19762ISP 6yesName: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
19762ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes

culture temp

@refgrowthtypetemperature
19762positiveoptimum34
7463positivegrowth34
32244positivegrowth28-45
32244positiveoptimum37
67770positivegrowth37
67771positivegrowth37

culture pH

@refabilitytypepH
32244positivegrowth06-08
32244positiveoptimum7.5

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
32244aerobe
67771aerobe

spore formation

@refspore formationconfidence
32244no
69481no99

halophily

@refsaltgrowthtested relationconcentration
32244NaClpositivegrowth0-10 %
32244NaClpositiveoptimum2.5 %

murein

  • @ref: 7463
  • murein short key: A31
  • type: A1gamma m-Dpm-direct

observation

  • @ref: 67770
  • observation: quinones: MK-8(H2), MK-7(H2)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3224416449alanine+carbon source
3224433984fucose+carbon source
3224424265gluconate+carbon source
3224425115malate+carbon source
3224429864mannitol+carbon source
3224437684mannose+carbon source
3224417268myo-inositol+carbon source
32244506227N-acetylglucosamine+carbon source
3224417148putrescine+carbon source
3224417814salicin+carbon source
3224430031succinate+carbon source
3224427082trehalose+carbon source
3224453426tween 80+carbon source
6837917632nitrate-reduction
683794853esculin-hydrolysis
6837916199urea-hydrolysis
683795291gelatin+hydrolysis
6837917634D-glucose-fermentation
6837916988D-ribose-fermentation
6837965327D-xylose-fermentation
6837916899D-mannitol-fermentation
6837917306maltose-fermentation
6837917716lactose-fermentation
6837917992sucrose-fermentation
6837928087glycogen-fermentation

enzymes

@refvalueactivityec
32244acid phosphatase+3.1.3.2
32244alkaline phosphatase+3.1.3.1
32244catalase+1.11.1.6
32244gelatinase+
32244pyrazinamidase+3.5.1.B15
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase-
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase+3.4.11.3
68382valine arylamidase+
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase+3.1.3.1
68379gelatinase+
68379urease-3.5.1.5
68379beta-glucosidase-3.2.1.21
68379N-acetyl-beta-glucosaminidase+3.2.1.52
68379alpha-glucosidase+3.2.1.20
68379beta-galactosidase+3.2.1.23
68379beta-glucuronidase+3.2.1.31
68379alkaline phosphatase+3.1.3.1
68379pyrrolidonyl arylamidase+3.4.19.3
68379pyrazinamidase-3.5.1.B15

API coryne

@refNITPYZPYRAPALbeta GURbeta GALalpha GLUbeta NAGESCUREGELGLURIBXYLMANMALLACSACGLYG
19762--++++++--+--------

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
19762+++-+++--+---------

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
7463saline alkali soilXinjiang ProvinceChinaCHNAsia
67770Saline soilXinjiangChinaCHNAsia
67771From saline-alkali soilXinjiang ProvinceChinaCHNAsia

isolation source categories

Cat1Cat2Cat3
#Environmental#Terrestrial#Soil
#Condition#Alkaline
#Condition#Saline

taxonmaps

  • @ref: 69479
  • File name: preview.99_5938.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_15817;96_2836;97_3488;98_4414;99_5938&stattab=map
  • Last taxonomy: Brevibacterium album subclade
  • 16S sequence: EF158852
  • Sequence Identity:
  • Total samples: 1093
  • soil counts: 197
  • aquatic counts: 167
  • animal counts: 683
  • plant counts: 46

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
197621Risk group (German classification)
74631Risk group (German classification)

Sequence information

16S sequences

  • @ref: 7463
  • description: Brevibacterium album strain YIM 90718 16S ribosomal RNA gene, partial sequence
  • accession: EF158852
  • length: 1532
  • database: nuccore
  • NCBI tax ID: 1121122

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Brevibacterium album DSM 182611121122.3wgspatric1121122
66792Brevibacterium album DSM 182612524614662draftimg1121122
67770Brevibacterium album DSM 18261 DSM 18261GCA_000426445scaffoldncbi1121122

GC content

@refGC-contentmethod
746370
6777070high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

@reftraitmodeldescriptionpredictionconfidencetraining_data
69481spore-formingspore-formingAbility to form endo- or exosporesno99no
69480gram-positivegram-positivePositive reaction to Gram-stainingyes87.524yes
69480anaerobicanaerobicAbility to grow under anoxygenic conditions (including facultative anaerobes)no98.568no
69480spore-formingspore-formingAbility to form endo- or exosporesno76.223no
69480aerobicaerobicAbility to grow under oxygenic conditions (including facultative aerobes)yes84.157yes
69480thermophilicthermophileAbility to grow at temperatures above or equal to 45°Cno92.929yes
69480flagellatedmotile2+Ability to perform flagellated movementno90.5no

External links

@ref: 7463

culture collection no.: DSM 18261, CCTCC AB 206112, KCTC 19173, YIM 90718, JCM 15617

straininfo link

  • @ref: 71513
  • straininfo: 397746

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny18319457Brevibacterium album sp. nov., a novel actinobacterium isolated from a saline soil in China.Tang SK, Wang Y, Schumann P, Stackebrandt E, Lou K, Jiang CL, Xu LH, Li WJInt J Syst Evol Microbiol10.1099/ijs.0.65183-02008Bacterial Typing Techniques, Brevibacterium/chemistry/*classification/genetics/isolation & purification, China, DNA, Bacterial/analysis, DNA, Ribosomal/analysis, Fatty Acids/analysis, Genes, rRNA, Molecular Sequence Data, Phenotype, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Sodium Chloride/chemistry, Soil/*analysis, *Soil Microbiology, Species SpecificityGenetics
Phylogeny20118289Brevibacterium salitolerans sp. nov., an actinobacterium isolated from salt-lake sediment.Guan TW, Zhao K, Xiao J, Liu Y, Xia ZF, Zhang XP, Zhang LLInt J Syst Evol Microbiol10.1099/ijs.0.020214-02010Bacterial Typing Techniques, Base Composition, Brevibacterium/*classification/genetics/isolation & purification, China, DNA, Bacterial/genetics, Diaminopimelic Acid/chemistry, Fatty Acids/chemistry, Geologic Sediments/*microbiology, Lakes/*microbiology, Molecular Sequence Data, Nucleic Acid Hybridization, Phospholipids/chemistry, *Phylogeny, RNA, Ribosomal, 16S/genetics, Salinity, Sequence Analysis, DNA, Vitamin K 2/analogs & derivatives/chemistryGenetics
Phylogeny22368170Brevibacterium daeguense sp. nov., a nitrate-reducing bacterium isolated from a 4-chlorophenol enrichment culture.Cui Y, Kang MS, Woo SG, Jin L, Kim KK, Park J, Lee M, Lee STInt J Syst Evol Microbiol10.1099/ijs.0.038141-02012Amino Acids/analysis, Bacterial Typing Techniques, Base Composition, Brevibacterium/*classification/genetics/isolation & purification/metabolism, Carbohydrates/analysis, *Chlorophenols, DNA, Bacterial/genetics, Fatty Acids/analysis, Molecular Sequence Data, Nitrates/*metabolism, Nucleic Acid Hybridization, *Phylogeny, RNA, Ribosomal, 16S/genetics, Republic of Korea, Sequence Analysis, DNA, Vitamin K 2/analogs & derivatives/analysisMetabolism

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmed
7463Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 18261)https://www.dsmz.de/collection/catalogue/details/culture/DSM-18261
19762Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM18261.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
32244Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2848528776041
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
67771Curators of the KCTChttps://kctc.kribb.re.kr/En/Kctc
68379Automatically annotated from API Coryne
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
71513Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID397746.1StrainInfo: A central database for resolving microbial strain identifiers