Strain identifier

BacDive ID: 18610

Type strain: No

Species: Nannocystis exedens

Strain Designation: Na e5

NCBI tax ID(s): 54 (species)

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General

@ref: 18281

BacDive-ID: 18610

keywords: Bacteria

description: Nannocystis exedens Na e5 is a bacterium that builds a fruiting body and was isolated from soil from grain field.

NCBI tax id

  • NCBI tax id: 54
  • Matching level: species

doi: 10.13145/bacdive18610.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Deltaproteobacteria
  • order: Myxococcales
  • family: Nannocystaceae
  • genus: Nannocystis
  • species: Nannocystis exedens
  • full scientific name: Nannocystis exedens Reichenbach 1970 (Approved Lists 1980)

@ref: 18281

domain: Bacteria

phylum: Proteobacteria

class: Deltaproteobacteria

order: Myxococcales

family: Nannocystaceae

genus: Nannocystis

species: Nannocystis exedens

full scientific name: Nannocystis exedens

strain designation: Na e5

type strain: no

Morphology

multicellular morphology

  • @ref: 18281
  • forms multicellular complex: yes
  • complex name: fruiting body

Culture and growth conditions

culture medium

@refnamegrowthcomposition
18281CHITIN 3 AGARnoIngredients: Yeast extract (Difco) 0.05% MgSO4 x 7H2O 0.1% Agar 1.2% Preparation / Remarks: pH 7.2 After autoclaving add 10 mL of sterile Chitin suspension (100 g/L Chitin in H2O) to 100 mL of the medium and pour on cy-plates.
18281CHITIN 4 AGARyesIngredients: Yeast extract (Difco) 0.05% MgSO4 x 7H2O 0.1% Agar 1.2% Preparation / Remarks: pH 7.2 After autoclaving add 10 mL of sterile Chitin suspension (100 g/L Chitin in H2O) to 100 mL of the medium and pour on w-agar plates.
18281COLE AGARyesIngredients: CaCl2 x 2 H2O 1.3g Agar (Difco) 15.0g Distilled water 1000.0ml Preparation / Remarks: pH 7.2 (KOH) autoclaved Pour the plates and streak living E.coli cells
18281SKIM MILK 1 AGARnoIngredients: cy-Agar 300 ml Skim milk (Oxoid) 15g H2O 60 ml Preparation / Remarks: 300 ml cy-agar autoclaved. Separately 15 g skim milk (Oxoid) in 60 ml water and exactly 15 min autoclaving at 115 °C. Mix the two and pour a thin layer on cy-plates.
18281TOLI AGARyesIngredients: CaCl2 x 2 H2O1.3g Agar (Difco)15.0g Distilled water1000.0ml Preparation / Remarks: pH 7.2 (KOH) autoclaved Pour the plates and streak autoclaved E.coli cells
18281VY/2 AGARyesIngredients: Baker's yeast 0.5% (based on fresh weight) CaCl2 0.1% Agar 1.5% Vitamin B12 0.05 % Preparation / Remarks: pH 7.2 autoclaved
18281XYLAN 1 AGARnoIngredients: Xylan 0.5% (NH4)2SO4 0.1% Agar 1% Preparation / Remarks: pH 7.2 Xylan (Serva pure molecular weight 25000) Pour after autoclaving as a thin layer on standard 6-agar

Physiology and metabolism

observation

@refobservation
18281Strong ability to Yeast lysis
18281No ability for chitin degradation
18281No ability for Yeast lysis
18281Ability for Yeast lysis
18281Ability for E. coli lysis

Isolation, sampling and environmental information

isolation

  • @ref: 18281
  • sample type: soil from grain field
  • sampling date: 1968-05-01
  • geographic location: Nebraska
  • country: USA
  • origin.country: USA
  • continent: North America

isolation source categories

Cat1Cat2Cat3
#Engineered#Agriculture#Field
#Environmental#Terrestrial#Soil
#Host#Plants#Herbaceous plants (Grass,Crops)

External links

@ref: 18281

Reference

@idauthorstitledoi/urljournalpubmed
18281Reichenbach, H.Collection description and documentation of myxobacteria by H. Reichenbach, HZI (formerly GBF); Collection curr. located at the DSMZ
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar SchomburgBRENDA, the ELIXIR core data resource in 2021: new developments and updates10.1093/nar/gkaa1025Nucleic Acids Res. 49: D498-D508 202033211880