Strain identifier

BacDive ID: 18488

Type strain: No

Species: unclassified

Strain Designation: Hy m22

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General

@ref: 18281

BacDive-ID: 18488

description: unclassified Hy m22 is a bacterium that was isolated from soil with plant residues.

doi: 10.13145/bacdive18488.20230509.8.1

Name and taxonomic classification

@ref: 18281

domain: Bacteria

phylum: unclassified

class: unclassified

order: unclassified

family: unclassified

genus: Not assigned to family

species: unclassified

strain designation: Hy m22

type strain: no

Culture and growth conditions

culture medium

@refnamegrowthcomposition
18281COLE AGARyesIngredients: CaCl2 x 2 H2O 1.3g Agar (Difco) 15.0g Distilled water 1000.0ml Preparation / Remarks: pH 7.2 (KOH) autoclaved Pour the plates and streak living E.coli cells
18281TOLI AGARyesIngredients: CaCl2 x 2 H2O1.3g Agar (Difco)15.0g Distilled water1000.0ml Preparation / Remarks: pH 7.2 (KOH) autoclaved Pour the plates and streak autoclaved E.coli cells
18281TOLI AGAR Kan4yesIngredients: Agar (Difco) 1.5% CaCl2 0.1% Cycloheximide 2.5 mg per 100 ml Preparation / Remarks: pH 7.2 (KOH) autoclaved w-agar with 2.5 mg of cycloheximide per 100 ml, the antibiotic is filter sterilized and added to the agar just before pouring Cycloheximide does not seem to affect growth of myxobakteria, whereas fungi and amoebae are often inhibited. Combine with 250 mg/L Kanamycin-sulfate (sterilized by filtration) Pour the plates and streak autoclaved E.coli cells
18281VY/2 AGARyesIngredients: Baker's yeast 0.5% (based on fresh weight) CaCl2 0.1% Agar 1.5% Vitamin B12 0.05 % Preparation / Remarks: pH 7.2 autoclaved

Physiology and metabolism

observation

@refobservation
18281Ability for Yeast lysis
18281Ability for E. coli lysis

antibiotic resistance

@refChEBIgroup IDmetaboliteis antibioticis resistantresistance conc.
1828161043kanamycinyesyes250 µg/mL
18281276413cycloheximideyesyes25 µg/mL

Isolation, sampling and environmental information

isolation

  • @ref: 18281
  • sample type: soil with plant residues
  • sampling date: 1999-04-01
  • geographic location: Rhodos Island
  • country: Greece
  • origin.country: GRC
  • continent: Europe

isolation source categories

Cat1Cat2Cat3
#Environmental#Terrestrial#Soil
#Host#Plants

External links

@ref: 18281

Reference

@idauthorstitle
18281Reichenbach, H.Collection description and documentation of myxobacteria by H. Reichenbach, HZI (formerly GBF); Collection curr. located at the DSMZ