Strain identifier

BacDive ID: 183

Type strain: Yes

Species: Pauljensenia hongkongensis

Strain Designation: HKU8

Strain history: CIP <- 2003, K.Y. Yuen, Hong Kong Univ., Hong Kong, China: strain HKU8

NCBI tax ID(s): 178339 (species)

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General

@ref: 5918

BacDive-ID: 183

DSM-Number: 15629

keywords: genome sequence, 16S sequence, Bacteria, anaerobe, mesophilic, Gram-positive, rod-shaped

description: Pauljensenia hongkongensis HKU8 is an anaerobe, mesophilic, Gram-positive bacterium that was isolated from pus of a patient with pelvic actinomycosis.

NCBI tax id

  • NCBI tax id: 178339
  • Matching level: species

strain history

@refhistory
5918<- P. C. Y. Woo; HKU8
399642003, K.Y. Yuen, Univ. of Hong Kong, Hong Kong: strain HKU8
119964CIP <- 2003, K.Y. Yuen, Hong Kong Univ., Hong Kong, China: strain HKU8

doi: 10.13145/bacdive183.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Actinomycetales
  • family: Actinomycetaceae
  • genus: Pauljensenia
  • species: Pauljensenia hongkongensis
  • full scientific name: Pauljensenia hongkongensis (Woo et al. 2004) Nouioui et al. 2018

  • synonyms

    • @ref: 20215
    • synonym: Actinomyces hongkongensis

@ref: 5918

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Actinomycetaceae

genus: Pauljensenia

species: Pauljensenia hongkongensis

full scientific name: Pauljensenia hongkongensis (Woo et al. 2004) Nouioui et al. 2018

strain designation: HKU8

type strain: yes

Morphology

cell morphology

  • @ref: 119964
  • gram stain: positive
  • cell shape: rod-shaped
  • motility: no

colony morphology

  • @ref: 119964

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
5918COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
39964MEDIUM 45 - for Columbia agar with sheep bloodyesDistilled water make up to (1000.000 ml);Columbia agar (39.000 g);Sheepblood (100.000 ml)
119964CIP Medium 45yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=45

culture temp

@refgrowthtypetemperaturerange
5918positivegrowth37mesophilic
39964positivegrowth37
58185positivegrowth37

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
5918anaerobe
58185anaerobe
119964anaerobe

halophily

@refsaltgrowthtested relationconcentration
119964NaClnogrowth0 %
119964NaClnogrowth2 %
119964NaClnogrowth4 %
119964NaClnogrowth6 %
119964NaClnogrowth8 %
119964NaClnogrowth10 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
11996416947citrate-carbon source
1199644853esculin-hydrolysis
119964606565hippurate+hydrolysis
11996417632nitrate-reduction
11996416301nitrite-reduction
11996417632nitrate+respiration
6838016199urea-hydrolysis
6838029016arginine+hydrolysis
6838016024D-mannose-fermentation
6838016634raffinose-fermentation
6838029985L-glutamate-degradation
6838017632nitrate-reduction
6838027897tryptophan-energy source

metabolite production

@refChebi-IDmetaboliteproduction
11996435581indoleno
6838035581indoleno

metabolite tests

@refChebi-IDmetaboliteindole testvoges-proskauer-testmethylred-test
6838035581indole-
11996415688acetoin-
11996417234glucose+

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase-
68382acid phosphatase-3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase-3.1.3.1
68380glutamyl-glutamate arylamidase-
68380tyrosine arylamidase+
68380pyrrolidonyl arylamidase-3.4.19.3
68380leucine arylamidase+3.4.11.1
68380phenylalanine arylamidase+
68380leucyl glycin arylamidase-3.4.11.1
68380proline-arylamidase+3.4.11.5
68380alkaline phosphatase-3.1.3.1
68380tryptophan deaminase-4.1.99.1
68380alpha-fucosidase-3.2.1.51
68380glutamate decarboxylase-4.1.1.15
68380N-acetyl-beta-glucosaminidase-3.2.1.52
68380beta-glucuronidase-3.2.1.31
68380alpha-arabinosidase-3.2.1.55
68380beta-glucosidase-3.2.1.21
68380alpha-glucosidase-3.2.1.20
68380beta-Galactosidase 6-phosphate-
68380beta-galactosidase-3.2.1.23
68380alpha-galactosidase-3.2.1.22
68380arginine dihydrolase+3.5.3.6
68380urease-3.5.1.5
119964oxidase-
119964beta-galactosidase+3.2.1.23
119964alcohol dehydrogenase+1.1.1.1
119964gelatinase-
119964amylase-
119964DNase-
119964caseinase-3.4.21.50
119964catalase-1.11.1.6
119964tween esterase-
119964gamma-glutamyltransferase+2.3.2.2
119964lecithinase-
119964lipase-
119964lysine decarboxylase+4.1.1.18
119964ornithine decarboxylase-4.1.1.17
119964phenylalanine ammonia-lyase-4.3.1.24
119964protease-
119964tryptophan deaminase-
119964urease-3.5.1.5

fatty acid profile

  • fatty acids

    @reffatty acidpercentageECL
    58185C16:031.916
    58185C18:014.218
    58185C18:1 ω9c30.817.769
    58185C18:2 ω6,9c/C18:0 ANTE23.117.724
  • type of FA analysis: whole cell analysis
  • method/protocol: CCUG

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
119964--++-+--------------

API rID32A

@refUREADH Argalpha GALbeta GALbeta GPalpha GLUbeta GLUalpha ARAbeta GURbeta NAGMNERAFGDCalpha FUCNITINDPALArgAProALGAPheALeuAPyrATyrAAlaAGlyAHisAGGASerA
5918-+---------------++-++-++++-+
5918-+---------------+/-+-++-+-----

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinentlatitudelongitudegeographic location
5918pus of a patient with pelvic actinomycosisHong KongHKGAsia22.3027114.177
58185Human pelvic actinomycosisChinaCHNAsiaHong Kong
119964Human, PusChinaCHNAsiaHong Kong

isolation source categories

Cat1Cat2
#Infection#Disease
#Infection#Patient
#Host Body-Site#Other
#Host Body Product#Fluids

taxonmaps

  • @ref: 69479
  • File name: preview.99_164533.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_224;96_61687;97_79918;98_108098;99_164533&stattab=map
  • Last taxonomy: Actinomycetaceae
  • 16S sequence: AF433168
  • Sequence Identity:
  • Total samples: 8086
  • soil counts: 221
  • aquatic counts: 69
  • animal counts: 7781
  • plant counts: 15

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
59182Risk group (German classification)
1199642Risk group (French classification)

Sequence information

16S sequences

  • @ref: 20218
  • description: Actinomyces hongkongensis 16S ribosomal RNA, partial sequence
  • accession: AF433168
  • length: 1425
  • database: ena
  • NCBI tax ID: 178339

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Pauljensenia hongkongensis HKU8GCA_001746855chromosomencbi178339
66792Pauljensenia hongkongensis HKU82751185834completeimg178339

External links

@ref: 5918

culture collection no.: DSM 15629, CCUG 48484, CIP 107949, LMG 21939

straininfo link

  • @ref: 69865
  • straininfo: 131407

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
5918Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 15629)https://www.dsmz.de/collection/catalogue/details/culture/DSM-15629
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
39964Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/5516
58185Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 48484)https://www.ccug.se/strain?id=48484
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68380Automatically annotated from API rID32A
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69865Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID131407.1StrainInfo: A central database for resolving microbial strain identifiers
119964Curators of the CIPCollection of Institut Pasteur (CIP 107949)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20107949