Strain identifier

BacDive ID: 18276

Type strain: No

Species: Cystobacter fuscus

Strain Designation: Cb f35

NCBI tax ID(s): 43 (species)

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General

@ref: 18281

BacDive-ID: 18276

keywords: Bacteria

description: Cystobacter fuscus Cb f35 is a bacterium that builds a fruiting body and was isolated from soil with plant residues beneath coconut tree.

NCBI tax id

  • NCBI tax id: 43
  • Matching level: species

doi: 10.13145/bacdive18276.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Deltaproteobacteria
  • order: Myxococcales
  • family: Archangiaceae
  • genus: Cystobacter
  • species: Cystobacter fuscus
  • full scientific name: Cystobacter fuscus Schroeter 1886 (Approved Lists 1980)

@ref: 18281

domain: Bacteria

phylum: Proteobacteria

class: Deltaproteobacteria

order: Myxococcales

family: Cystobacteraceae

genus: Cystobacter

species: Cystobacter fuscus

full scientific name: Cystobacter fuscus

strain designation: Cb f35

type strain: no

Morphology

multicellular morphology

  • @ref: 18281
  • forms multicellular complex: yes
  • complex name: fruiting body

multimedia

@refmultimedia contentcaptionintellectual property rights
18281/dsmz_profile/Pictures_DSMZ_Web/Cystobacter_fuscus/Cb_f35Cb f35Helmholtz-Zentrum für Infektionsforschung GmbH
18281/dsmz_profile/Pictures_DSMZ_Web/Cystobacter_fuscus/Cb_f35Cb f35Helmholtz-Zentrum für Infektionsforschung GmbH

Culture and growth conditions

culture medium

@refnamegrowthcomposition
18281CA2 AGARyesIngredients: Stock solution 1 (in 100ml): KNO3 7.5g K2HPO4 7.5g Stock solution 2 (in 100ml): MgSO4 x 7H2O 1.5g Stock solution 3 (in 100ml): CaCl2 0.2g FeCl3 0.15g Basal medium: Agar 1.5% Preparation / Remarks: after autoclaving to: a) 1% (v/v) of the 3 stock solutions b) 1% glucose c) trace elements
18281CHITIN 3 AGARyesIngredients: Yeast extract (Difco) 0.05% MgSO4 x 7H2O 0.1% Agar 1.2% Preparation / Remarks: pH 7.2 After autoclaving add 10 mL of sterile Chitin suspension (100 g/L Chitin in H2O) to 100 mL of the medium and pour on cy-plates.
18281CY-AGARyesIngredients: Casitone(Difco) 0.3% yeast extract 0.1% CaCl2 0.1% Agar 1.5% Preparation / Remarks: pH 7.2 (KOH) autoclaved
18281HP17 AGARyesIngedients: Na-glutaminate 0.5% MgSO4x7H2O 0.1% Agar 1.5% autoclaved separately: Glucose 0.2% Preparation / Remarks: pH 7
18281HP18 AGARyesIngredients: Na-glutaminate 0.1% MgSO4x7H2O 0.1% Agar 1.5% Glucose 0.1% Preparation / Remarks: sterile traceelement-solution 0.1 ml (100 ml after autoclaving to, pH 7.2) Autoclaving glucose seperatly
18281Myx MEDIUMyesIngredients: Peptone (Difco) 1% Glucose 1% Yeast extract (Difco) 0.1% Phosphate buffer 0.01 M pH 6.5 Aerobacter aerogenes (sterilized with formalin) 500 mg/L (dry weight) as described by Kerr, N.S. (1963) The Growth of Myxamoebae of the True Slime Mould, Didymium nigripes, in Axenic Culture. J Gen Microbiol 32: 409-416.
18281PROBION L AGARyesIngredients: Probion L 600 (Hoechst) 0.8% MgSO4 x 7H2O 0.1% CaCl2 x 2H2O 0.1% Agar 1.5% Preparation / Remarks: pH 7.2 autoclaved
18281SKIM MILK 1 AGARyesIngredients: cy-Agar 300 ml Skim milk (Oxoid) 15g H2O 60 ml Preparation / Remarks: 300 ml cy-agar autoclaved. Separately 15 g skim milk (Oxoid) in 60 ml water and exactly 15 min autoclaving at 115 °C. Mix the two and pour a thin layer on cy-plates.
18281VY/2 AGARyesIngredients: Baker's yeast 0.5% (based on fresh weight) CaCl2 0.1% Agar 1.5% Vitamin B12 0.05 % Preparation / Remarks: pH 7.2 autoclaved

Physiology and metabolism

observation

@refobservation
18281Strong ability to Yeast lysis
18281Ability for chitin degradation
18281Ability for protein degradation
18281Ability for Yeast lysis

Isolation, sampling and environmental information

isolation

  • @ref: 18281
  • sample type: soil with plant residues beneath coconut tree
  • sampling date: 1987-03-01
  • geographic location: Lantar near Abeokuta
  • country: Nigeria
  • origin.country: NGA
  • continent: Africa

isolation source categories

Cat1Cat2Cat3
#Environmental#Terrestrial#Soil
#Host#Plants#Tree

External links

@ref: 18281

Reference

@idauthorstitledoi/url
18281Reichenbach, H.Collection description and documentation of myxobacteria by H. Reichenbach, HZI (formerly GBF); Collection curr. located at the DSMZ
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332