Strain identifier

BacDive ID: 18180

Type strain: No

Species: Herpetosiphon sp.

Strain Designation: Hp g12

NCBI tax ID(s): 71864 (species)

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General

@ref: 18281

BacDive-ID: 18180

keywords: Bacteria

description: Herpetosiphon sp. Hp g12 is a bacterium that was isolated from slimy mat of Chara sp. out of Birch Lake.

NCBI tax id

  • NCBI tax id: 71864
  • Matching level: species

doi: 10.13145/bacdive18180.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/chloroflexota
  • domain: Bacteria
  • phylum: Chloroflexota
  • class: Chloroflexia
  • order: Herpetosiphonales
  • family: Herpetosiphonaceae
  • genus: Herpetosiphon
  • species: Herpetosiphon sp.
  • full scientific name: Herpetosiphon Holt and Lewin 1968 (Approved Lists 1980)

@ref: 18281

domain: Bacteria

phylum: Chloroflexi

class: Chloroflexi, not validated

order: Herpetosiphonales

family: Herpetosiphonaceae, not validated

genus: Herpetosiphon

species: Herpetosiphon sp.

full scientific name: Herpetosiphon sp.

strain designation: Hp g12

type strain: no

Morphology

multimedia

  • @ref: 18281
  • multimedia content: /dsmz_profile/Pictures_DSMZ_Web/Herpetosiphon_giganteus/Hp_g12
  • caption: Hp g12
  • intellectual property rights: Helmholtz-Zentrum für Infektionsforschung GmbH

Culture and growth conditions

culture medium

@refnamegrowthcomposition
18281CHITIN 7 AGARyesCovering agar (thin layer): MgSO4 x 7H2O 0.1% K2HPO4 0.02% Agar 1.5% Preparation / Remarks: pH 7.2 After autoclaving add 30 mL of sterile Chitin suspension (22.5 g/L Chitin in H2O) to 100 mL of the medium and pour on w-agar plates
18281CY-AGARyesIngredients: Casitone(Difco) 0.3% yeast extract 0.1% CaCl2 0.1% Agar 1.5% Preparation / Remarks: pH 7.2 (KOH) autoclaved
18281KAN 5 AGARyesIngredients: Baker's yeast 0.5% (based on fresh weight) CaCl2 0.1% Agar 1.5% Vitamine B12 0.5 µg/ml Preparation / Remarks: pH 7.2 autoclaved Combine with 250 mg/L Kanamycin-sulfate (sterilized by filtration)
18281KAN 7 AGARnoIngredients: Baker's yeast 0.5% (based on fresh weight) CaCl2 0.1% Agar 1.5% Vitamin B12 0.05 % Preparation / Remarks: pH 7.2 autoclaved Combine with 250 mg/L Kanamycin-sulfate, 250 mg/L Neomycin-sulfate and 250 mg/L Cycloheximide (each sterilized by filtration)
18281Myx MEDIUMyesIngredients: Peptone (Difco) 1% Glucose 1% Yeast extract (Difco) 0.1% Phosphate buffer 0.01 M pH 6.5 Aerobacter aerogenes (sterilized with formalin) 500 mg/L (dry weight) as described by Kerr, N.S. (1963) The Growth of Myxamoebae of the True Slime Mould, Didymium nigripes, in Axenic Culture. J Gen Microbiol 32: 409-416.
18281STAN 21 AGAR with FilteryesIngredients: 1. Agar 1% K2HPO4 0.1% Yeast extract (Difco) 0.002% 2. (NH4)2SO4 0.1% MgSO4 x 7H2O 0.1% CaCl2 x 2H2O 0.1% MnSO4 x H2O 0.01% FeCl3 x 6H2O 0.02% MnSO4 x 7H2O 0.01% FeCl3 0.02% Traceelement-solution 1ml to 100 ml Preparation / Remarks: 1. in 2/3 of the volume after autoclaving 1 + 2 together and pour plates. Hang up a sterile-filter paper

Physiology and metabolism

observation

@refobservation
18281Ability for chitin degradation
18281Ability for Yeast lysis

antibiotic resistance

  • @ref: 18281
  • ChEBI: 6104
  • metabolite: kanamycin
  • is antibiotic: yes
  • is resistant: yes
  • resistance conc.: 250 µg/mL

Isolation, sampling and environmental information

isolation

  • @ref: 18281
  • sample type: slimy mat of Chara sp. out of Birch Lake
  • host species: Chara
  • geographic location: Minnesota
  • country: USA
  • origin.country: USA
  • continent: North America

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Lake (large)
#Host#Algae#Green algae

External links

@ref: 18281

Reference

@idauthorstitledoi/url
18281Reichenbach, H.Collection description and documentation of myxobacteria by H. Reichenbach, HZI (formerly GBF); Collection curr. located at the DSMZ
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ10.1099/ijsem.0.004332